PMID- 33902141
OWN - NLM
STAT- MEDLINE
DCOM- 20211028
LR  - 20220422
IS  - 2567-5834 (Electronic)
IS  - 1434-1220 (Linking)
VI  - 49
IP  - 2
DP  - 2021 Apr
TI  - [Comparison of sample materials for the detection of infection with porcine 
      reproductive and respiratory syndrome virus].
PG  - 112-119
LID - 10.1055/a-1385-5982 [doi]
AB  - OBJECTIVE: In breeding farms with a high health status, pigs are frequently 
      sampled using invasive methods (i. e. blood sampling). The aim of the present 
      study was to evaluate less invasive methods concerning their suitability for an 
      early detection of the infection with the porcine reproductive and respiratory 
      syndrome virus (PRRSV). MATERIAL AND METHODS: Blood and saliva swabs, chewing 
      rope derived oral fluids and serum samples were used for PRRSV-1 and PRRSV-2 
      detection via PCR. 19 gilts were repeatedly sampled following intramuscular or 
      intranasal vaccination with live-attenuated vaccines containing PRRSV-1 or -2. 
      Swabs were either moistened with saliva from the mouth mucosa or with blood from 
      the ear veins following superficial needle puncture. Serum samples were taken via 
      puncture of the V. jugularis externa. Chewing ropes served for a means of oral 
      fluid sampling and were kept hanging in the barn at the pigs' head height for 
      30 minutes. RESULTS: All animals were negative for PRRSV at the time of 
      vaccination (sampling point 0). In serum samples, the first virus detection was 
      achieved 12 hours following vaccination (p. vacc.). From day 4 p. vacc. on all 
      animals were viremic and from day 10 p. vacc. on the percentage of positive 
      animals decreased. The first detection of PRRSV in GenoTube((R)) blood swabs was 
      possible 36 hours p. vacc. Examination of the eSwab((R)) blood swab resulted in 
      only one positive finding within the entire testing period. In the saliva 
      samples, first detection of PRRSV was possible on day 5 p. vacc. CONCLUSION AND 
      CLINICAL RELEVANCE: This study demonstrates that serum samples taken from the 
      V. jugularis externa may be considered as the gold standard for diagnostic of 
      PRRSV viremia. Detection rates in serum were higher than in the alternative 
      sample types. However, since sample collection procedures and processing of the 
      alternative sample materials offer clear advantages with regard to welfare, 
      practical handling and logistics, further attempts are warranted in order to 
      improve these methods. Based on the presented results, the described method using 
      eSwab((R)) blood sampling does not represent a satisfactory alternative approach 
      for the detection of early PRRSV infection. Future application of this method 
      warrants further improvement of its diagnostic efficacy.
CI  - Thieme. All rights reserved.
FAU - Konighoff, Philipp
AU  - Konighoff P
AD  - Aussenstelle fur Epidemiologie, Bakum, Stiftung Tierarztliche Hochschule Hannover.
FAU - Grosse Beilage, Elisabeth
AU  - Grosse Beilage E
FAU - Menrath, Andrea
AU  - Menrath A
FAU - Hennig-Pauka, Isabel
AU  - Hennig-Pauka I
LA  - ger
PT  - Journal Article
TT  - Vergleich von Probenmaterialien zur Fruherkennung einer Infektion mit dem Virus 
      des Porzinen Reproduktiven und Respiratorischen Syndroms.
DEP - 20210426
PL  - Germany
TA  - Tierarztl Prax Ausg G Grosstiere Nutztiere
JT  - Tierarztliche Praxis. Ausgabe G, Grosstiere/Nutztiere
JID - 9715779
RN  - 0 (Antibodies, Viral)
RN  - 0 (Vaccines, Attenuated)
RN  - 0 (Viral Vaccines)
SB  - IM
MH  - Animals
MH  - Antibodies, Viral
MH  - Female
MH  - *Porcine Reproductive and Respiratory Syndrome/diagnosis
MH  - *Porcine respiratory and reproductive syndrome virus
MH  - Swine
MH  - *Swine Diseases
MH  - Vaccines, Attenuated
MH  - *Viral Vaccines
MH  - Viremia/diagnosis/veterinary
COIS- Die Autoren bestatigen, dass kein Interessenkonflikt besteht.
EDAT- 2021/04/27 06:00
MHDA- 2021/10/29 06:00
CRDT- 2021/04/26 20:32
PHST- 2021/04/26 20:32 [entrez]
PHST- 2021/04/27 06:00 [pubmed]
PHST- 2021/10/29 06:00 [medline]
AID - 10.1055/a-1385-5982 [doi]
PST - ppublish
SO  - Tierarztl Prax Ausg G Grosstiere Nutztiere. 2021 Apr;49(2):112-119. doi: 
      10.1055/a-1385-5982. Epub 2021 Apr 26.