PMID- 33905885
OWN - NLM
STAT- MEDLINE
DCOM- 20220118
LR  - 20220118
IS  - 1567-7257 (Electronic)
IS  - 1567-1348 (Linking)
VI  - 93
DP  - 2021 Sep
TI  - Dysregulation of autophagy-associated microRNAs in condyloma acuminatum.
PG  - 104878
LID - S1567-1348(21)00175-1 [pii]
LID - 10.1016/j.meegid.2021.104878 [doi]
AB  - Condyloma acuminatum, which is caused by low-risk human papillomavirus (lrHPV) 
      infection, is one of the most common sexually transmitted diseases. Autophagy is 
      thought to be associated with condyloma acuminatum, but how the autophagy process 
      is regulated remains unclear. MicroRNAs (miRNAs) are important regulators of gene 
      transcription that play a central role in many biological processes, including 
      autophagy and viral infection. This study was designed to identify 
      autophagy-related miRNAs and their targets in condyloma acuminatum and to 
      validate their expression. The levels of the autophagy proteins 
      microtubule-associated protein 1 light chain 3 (LC3) and P62/SQSTM1 (P62) were 
      abnormally increased in the local lesion tissue of condyloma acuminatum patients 
      compared with healthy controls. MiRNAs and their target mRNAs in condyloma 
      acuminatum patients were analyzed by bioinformatics. Eighty-one differentially 
      expressed miRNAs were identified, of which 56 were downregulated and 25 were 
      upregulated. Two of the differentially expressed miRNAs associated with 
      autophagy, miRNA-30a-5p and miRNA-514a-3p, were analyzed further, and their 
      target genes were identified as autophagy-related protein (Atg) 5 and Atg12 and 
      Atg3 and Atg12, respectively. The expression levels of miRNA-30a-5p and 
      miRNA-514a-3p were decreased and those of Atg5, Atg12 and Atg3 were increased in 
      condyloma acuminatum patients compared with healthy controls. In addition, 
      miRNA-30a-5p and miRNA-514a-3p expression correlated with the proliferation index 
      Ki-67 in condyloma acuminatum. Taken together, our results suggest that the 
      changes in autophagy levels in patients with condyloma acuminatum may be related 
      to the changes in miRNA-30a-5p and miRNA-514a-3p expression. This study provides 
      a theoretical basis for identifying new mechanisms that link miRNAs, HPV 
      infection and host autophagy in vivo.
CI  - Copyright (c) 2021. Published by Elsevier B.V.
FAU - Wu, Shi
AU  - Wu S
AD  - Department of Dermatology, The First Affiliated Hospital of Jinan University, 
      Jinan University, Guangzhou 510630, China; Dermatology Institute of Jinan 
      University, Jinan University, Guangzhou 510630, China.
FAU - Lu, Dan
AU  - Lu D
AD  - Department of Neurology and Stroke Center, The First Affiliated Hospital of Jinan 
      University, Jinan University, Guangzhou 510630, China; Clinical Neuroscience 
      Institute of Jinan University, Jinan University, Guangzhou 510630, China.
FAU - Zheng, Xinkai
AU  - Zheng X
AD  - Department of Dermatology, The First Affiliated Hospital of Jinan University, 
      Jinan University, Guangzhou 510630, China; Dermatology Institute of Jinan 
      University, Jinan University, Guangzhou 510630, China.
FAU - Xu, Jin
AU  - Xu J
AD  - Department of Dermatology, The First Affiliated Hospital of Jinan University, 
      Jinan University, Guangzhou 510630, China; Dermatology Institute of Jinan 
      University, Jinan University, Guangzhou 510630, China.
FAU - Li, Zhen
AU  - Li Z
AD  - Department of Dermatology, The First Affiliated Hospital of Jinan University, 
      Jinan University, Guangzhou 510630, China; Department of Laser Cosmetology, The 
      fifth people's hospital of Hainan Province, Haikou 570100, China.
FAU - Deng, Liehua
AU  - Deng L
AD  - Department of Dermatology, The First Affiliated Hospital of Jinan University, 
      Jinan University, Guangzhou 510630, China; Dermatology Institute of Jinan 
      University, Jinan University, Guangzhou 510630, China. Electronic address: 
      liehuadeng@126.com.
FAU - Hu, Yunfeng
AU  - Hu Y
AD  - Department of Dermatology, The First Affiliated Hospital of Jinan University, 
      Jinan University, Guangzhou 510630, China; Dermatology Institute of Jinan 
      University, Jinan University, Guangzhou 510630, China. Electronic address: 
      huyunfeng@jnu.edu.cn.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20210424
PL  - Netherlands
TA  - Infect Genet Evol
JT  - Infection, genetics and evolution : journal of molecular epidemiology and 
      evolutionary genetics in infectious diseases
JID - 101084138
RN  - 0 (MicroRNAs)
SB  - IM
MH  - Adult
MH  - Autophagy/*genetics
MH  - Condylomata Acuminata/*physiopathology/virology
MH  - Down-Regulation
MH  - Female
MH  - Genital Diseases/*physiopathology/virology
MH  - Humans
MH  - Male
MH  - MicroRNAs/*metabolism
MH  - Middle Aged
MH  - Papillomaviridae/physiology
MH  - Papillomavirus Infections/*physiopathology/virology
MH  - Young Adult
OTO - NOTNLM
OT  - Autophagy
OT  - Condyloma acuminate
OT  - Human papillomavirus
OT  - MicroRNA
EDAT- 2021/04/28 06:00
MHDA- 2022/01/19 06:00
CRDT- 2021/04/27 20:13
PHST- 2020/10/13 00:00 [received]
PHST- 2021/04/06 00:00 [revised]
PHST- 2021/04/18 00:00 [accepted]
PHST- 2021/04/28 06:00 [pubmed]
PHST- 2022/01/19 06:00 [medline]
PHST- 2021/04/27 20:13 [entrez]
AID - S1567-1348(21)00175-1 [pii]
AID - 10.1016/j.meegid.2021.104878 [doi]
PST - ppublish
SO  - Infect Genet Evol. 2021 Sep;93:104878. doi: 10.1016/j.meegid.2021.104878. Epub 
      2021 Apr 24.