PMID- 33906978
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20220426
IS  - 2005-3606 (Print)
IS  - 2005-5447 (Electronic)
IS  - 2005-3606 (Linking)
VI  - 14
IP  - 3
DP  - 2021 Aug 30
TI  - Exosomes Derived from Human Umbilical Cord Mesenchymal Stem Cells Regulate 
      Macrophage Polarization to Attenuate Systemic Lupus Erythematosus-Associated 
      Diffuse Alveolar Hemorrhage in Mice.
PG  - 331-340
LID - 10.15283/ijsc20156 [doi]
AB  - BACKGROUND AND OBJECTIVES: To investigate the effect and the underlying mechanism 
      of exosomes secreted by human umbilical cord mesenchymal stem cells (hUCMSCs) on 
      diffuse alveolar hemorrhage (DAH) in murine lupus. METHODS AND RESULTS: Exosomes 
      were extracted from cultured hUCMSCs by ultracentrifugation. The expressions of 
      exosome markers (Alix, CD63 and TSG101) were measured for identification of 
      hUCMSC-derived exosomes (hUCMSC-exosomes). The alveolar hemorrhage of DAH mice 
      was revealed by H&E staining. The primary alveolar macrophages were isolated from 
      bronchoalveolar lavage fluid (BALF) of DAH mice. The expressions of M1 macrophage 
      markers (iNOS, IL-6, TNF-alpha and IL-1beta) and M2 macrophage markers (Arg1, IL-10, 
      TGF-beta and chi3l3) were detected. Flow cytometry measured the ratio of M1/M2 
      macrophages. ELISA measured the secretion of pro-inflammatory cytokines (IL-6 and 
      TNF-alpha) and anti-inflammatory cytokines (IL-10 and TGF-beta). DAH mice had hemorrhage 
      and small-vessel vasculitis in the lung, with neutrophil and monocyte 
      infiltration observed around the capillary and small artery. Furthermore, 
      increases of IL-6 and TNF-alpha, and decreases of IL-10 and TGF-beta were detected in 
      the BALF of DAH mice. M1 makers were overexpressed in alveolar macrophages of DAH 
      mice while M2 makers were lowly expressed. DAH mice had a higher proportion of M1 
      macrophages than M2 macrophages. After hUCMSC-exosome or methylprednisolone 
      treatment in DAH mice, the alveolar injuries and inflammatory responses were 
      attenuated, and the proportion of M2 macrophages was increased. CONCLUSIONS: 
      hUCMSC-exosomes attenuate DAH-induced inflammatory responses and alveolar 
      hemorrhage by regulating macrophage polarization.
FAU - Chen, Xun
AU  - Chen X
AD  - Department of Pediatrics, The First Affiliated Hospital of Guangxi Medical 
      University, Guangxi, China.
FAU - Wei, Qing
AU  - Wei Q
AD  - Department of Pediatrics, The First Affiliated Hospital of Guangxi Medical 
      University, Guangxi, China.
FAU - Sun, Hongmei
AU  - Sun H
AD  - Department of Pediatrics, The First Affiliated Hospital of Guangxi Medical 
      University, Guangxi, China.
FAU - Zhang, Xiaobo
AU  - Zhang X
AD  - Department of Pediatrics, The First Affiliated Hospital of Guangxi Medical 
      University, Guangxi, China.
FAU - Yang, Changrong
AU  - Yang C
AD  - Department of Pediatrics, The First Affiliated Hospital of Guangxi Medical 
      University, Guangxi, China.
FAU - Tao, Ying
AU  - Tao Y
AD  - Department of Pediatrics, The First Affiliated Hospital of Guangxi Medical 
      University, Guangxi, China.
FAU - Nong, Guangmin
AU  - Nong G
AD  - Department of Pediatrics, The First Affiliated Hospital of Guangxi Medical 
      University, Guangxi, China.
LA  - eng
PT  - Journal Article
PL  - Korea (South)
TA  - Int J Stem Cells
JT  - International journal of stem cells
JID - 101497587
PMC - PMC8429939
OTO - NOTNLM
OT  - Diffuse alveolar hemorrhage
OT  - Exosome
OT  - Human umbilical cord mesenchymal stem cells
OT  - M1 macrophage
OT  - M2 macrophage
OT  - Systemic lupus erythematosus
COIS- Potential Conflict of Interest The authors have no conflicting financial 
      interest.
EDAT- 2021/04/29 06:00
MHDA- 2021/04/29 06:01
PMCR- 2021/04/30
CRDT- 2021/04/28 05:46
PHST- 2020/09/28 00:00 [received]
PHST- 2020/12/24 00:00 [revised]
PHST- 2021/01/28 00:00 [accepted]
PHST- 2021/04/29 06:00 [pubmed]
PHST- 2021/04/29 06:01 [medline]
PHST- 2021/04/28 05:46 [entrez]
PHST- 2021/04/30 00:00 [pmc-release]
AID - ijsc20156 [pii]
AID - ijsc-14-3-331 [pii]
AID - 10.15283/ijsc20156 [doi]
PST - ppublish
SO  - Int J Stem Cells. 2021 Aug 30;14(3):331-340. doi: 10.15283/ijsc20156.