PMID- 33911952 OWN - NLM STAT- PubMed-not-MEDLINE LR - 20210430 IS - 1319-562X (Print) IS - 2213-7106 (Electronic) IS - 2213-7106 (Linking) VI - 28 IP - 4 DP - 2021 Apr TI - Molecular mechanisms underlying antitumor activity of camel whey protein against multiple myeloma cells. PG - 2374-2380 LID - 10.1016/j.sjbs.2021.01.034 [doi] AB - Treating drug-resistant cancer cells is a clinical challenge and it is also vital to screen for new cancer drugs. Multiple myeloma (MM) is a plasma cell clonal cancer that, despite many experimental therapeutics, remains incurable. In this study, two MM cell line lines U266 and RPMI 8226 were used to determine the impact of camel whey protein (CWP). The CWP IC(50) was calculated by MTT examination, while the flow cytometry analysis was used to investigate the chemotaxis responses of MM cells in relation to CXCL12 and the pro-apoptotic effect of CHP. MM cells were treated with CWP and Western blot analysis was used to determine the underlying molecular mechanisms. Dose and time based on the impact of CWP on the cell viability of MM cells with IC(50) of 50 mug/ml, without affecting the viability of normal healthy PBMCs. CWP reduced chemotaxis of MM cells significantly from the CXC chemokine ligand 12 (CXCL12). Using Western blot analysis, we found that CWP decreased the activation of AKT, mTOR, PLCbeta3, NFalphaB and ERK, which was mechanistically mediated by CXCL12/CXCR4. In both U266 and RPMI 8226, CWP induced apoptosis by upregulating cytochrome C expression. In addition, CWP mediated the growth arrest of MM cells by robustly decreasing the expression of the anti-apoptotic Bcl-2 family members Bcl-2, Bcl-XL and Mcl-1. Conversely, the expression of pro-apoptotic Bcl-2 family members Bak, Bax and Bim was increased after treatment with CWP. Our data indicates CWP's therapeutic potential for MM cells. CI - (c) 2021 The Author(s). FAU - Badr, Gamal AU - Badr G AD - Zoology Department, Faculty of Science, Assiut University, 71516 Assiut, Egypt. AD - Laboratory of Immunology, Zoology Department, Faculty of Science, Assiut University, 71516 Assiut, Egypt. FAU - Sayed, Eman Abdo AU - Sayed EA AD - Zoology Department, Faculty of Science, Assiut University, 71516 Assiut, Egypt. AD - Laboratory of Immunology, Zoology Department, Faculty of Science, Assiut University, 71516 Assiut, Egypt. FAU - Abdel-Ghaffar, Wafaa H AU - Abdel-Ghaffar WH AD - Department of Zoology, Faculty of Science, Ain Shams University, Cairo, Egypt. FAU - Badr, Badr M AU - Badr BM AD - Radiation Biology Department, National Centre for Radiation Research and Technology (NCRRT), Cairo, Egypt. FAU - Sayed, Leila H AU - Sayed LH AD - Zoology Department, Faculty of Science, Assiut University, 71516 Assiut, Egypt. AD - Laboratory of Immunology, Zoology Department, Faculty of Science, Assiut University, 71516 Assiut, Egypt. FAU - Sayed, Aml AU - Sayed A AD - Mallawy Hospital, 23 Of July Street, Mallawy, Minya, Egypt. FAU - Mahmoud, Mohamed H AU - Mahmoud MH AD - Department of Biochemistry, College of Science, King Saud University, PO Box 22452, Riyadh 11451, Saudi Arabia. FAU - Alamery, Salman AU - Alamery S AD - Department of Biochemistry, College of Science, King Saud University, PO Box 22452, Riyadh 11451, Saudi Arabia. LA - eng PT - Journal Article DEP - 20210128 PL - Saudi Arabia TA - Saudi J Biol Sci JT - Saudi journal of biological sciences JID - 101543796 PMC - PMC8071924 OTO - NOTNLM OT - Apoptosis OT - CWP OT - Cell signage OT - Family members Bcl-2 OT - Myeloma multiple COIS- The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. EDAT- 2021/04/30 06:00 MHDA- 2021/04/30 06:01 PMCR- 2021/01/28 CRDT- 2021/04/29 06:24 PHST- 2020/11/26 00:00 [received] PHST- 2021/01/09 00:00 [revised] PHST- 2021/01/14 00:00 [accepted] PHST- 2021/04/29 06:24 [entrez] PHST- 2021/04/30 06:00 [pubmed] PHST- 2021/04/30 06:01 [medline] PHST- 2021/01/28 00:00 [pmc-release] AID - S1319-562X(21)00034-6 [pii] AID - 10.1016/j.sjbs.2021.01.034 [doi] PST - ppublish SO - Saudi J Biol Sci. 2021 Apr;28(4):2374-2380. doi: 10.1016/j.sjbs.2021.01.034. Epub 2021 Jan 28.