PMID- 33912959
OWN - NLM
STAT- MEDLINE
DCOM- 20211222
LR  - 20211222
IS  - 1349-9157 (Electronic)
IS  - 0449-3060 (Print)
IS  - 0449-3060 (Linking)
VI  - 62
IP  - 4
DP  - 2021 Jul 10
TI  - Resveratrol protects intestinal epithelial cells against radiation-induced damage 
      by promoting autophagy and inhibiting apoptosis through SIRT1 activation.
PG  - 574-581
LID - 10.1093/jrr/rrab035 [doi]
AB  - Intrinsic autophagy is important for the maintenance of intestinal homeostasis 
      and intestinal regeneration. Ionizing radiation suppresses intrinsic autophagy 
      and reduces damage-induced regeneration in the intestine, resulting in intestinal 
      injury. Resveratrol, a sirtuin 1 (SIRT1) agonist, promotes autophagy and exerts 
      radioprotective effect. In this study, the protective effect of resveratrol 
      against radiation-induced intestinal injury and its potential mechanism were 
      investigated. Intestinal epithelial cells (IEC-6) were exposed to 10 Gy ionizing 
      radiation and resveratrol (0.1-40.0 muM). Cell viability was investigated using 
      Cell Counting Kit 8 (CCK8), apoptosis was observed by Annexin V-fluorescein 
      isothiocyanate/propidium iodide (PI) staining and flow cytometry, and the 
      expression of apoptotic and autophagic proteins was determined by western 
      blotting. Resveratrol exerted a high toxicity against IEC-6 cells, but at low 
      concentrations, it inhibited ionizing radiation-induced apoptosis. Resveratrol 
      increased SIRT1 expression after irradiation and inhibited ionizing 
      radiation-induced p53 acetylation and pro-apoptotic protein, Bax, expression. 
      Furthermore, resveratrol promoted autophagy via the phosphoinositide 3-kinase 
      (PI3K)/AKT/mammalian target of rapamycin (mTOR) pathway, thereby protecting IEC-6 
      cells against radiation-induced damage. These results suggest that resveratrol 
      reduces radiation-induced IEC-6 cell damage by inhibiting apoptosis and promoting 
      autophagy via the activation of SIRT1, and that the PI3K/AKT/mTOR signaling 
      pathway is involved in the induction of autophagy.
CI  - (c) The Author(s) 2021. Published by Oxford University Press on behalf of The 
      Japanese Radiation Research Society and Japanese Society for Radiation Oncology.
FAU - Qin, Haoren
AU  - Qin H
AD  - Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China.
FAU - Zhang, Heng
AU  - Zhang H
AD  - Tianjin Union Medical Center of Nankai University, Department of Oncology, 
      Tianjin 300121, China.
FAU - Zhang, Xipeng
AU  - Zhang X
AD  - Tianjin Union Medical Center of Nankai University, Department of Colorectal 
      Surgery, Tianjin 300121, China.
FAU - Zhang, Shiwu
AU  - Zhang S
AD  - Tianjin Union Medical Center of Nankai University, Department of Pathology, 
      Tianjin 300121, China.
FAU - Zhu, Siwei
AU  - Zhu S
AD  - Tianjin Union Medical Center of Nankai University, Department of Oncology, 
      Tianjin 300121, China.
FAU - Wang, Hui
AU  - Wang H
AD  - Tianjin Union Medical Center of Nankai University, Department of Oncology, 
      Tianjin 300121, China.
LA  - eng
GR  - 81972847/National Natural Science Foundation of China/
PT  - Journal Article
PL  - England
TA  - J Radiat Res
JT  - Journal of radiation research
JID - 0376611
RN  - 0 (Beclin-1)
RN  - 0 (Map1lc3b protein, mouse)
RN  - 0 (Microtubule-Associated Proteins)
RN  - 0 (Tumor Suppressor Protein p53)
RN  - EC 2.7.11.1 (TOR Serine-Threonine Kinases)
RN  - EC 3.5.1.- (Sirtuin 1)
RN  - Q369O8926L (Resveratrol)
SB  - IM
MH  - Acetylation/drug effects/radiation effects
MH  - Animals
MH  - *Apoptosis/drug effects/radiation effects
MH  - *Autophagy/drug effects/radiation effects
MH  - Beclin-1/metabolism
MH  - Cell Line
MH  - Cell Survival/drug effects/radiation effects
MH  - *Cytoprotection/drug effects/radiation effects
MH  - Enterocytes/drug effects/*pathology/radiation effects
MH  - Mice
MH  - Microtubule-Associated Proteins/metabolism
MH  - Models, Biological
MH  - *Radiation, Ionizing
MH  - Resveratrol/*pharmacology
MH  - Signal Transduction/drug effects
MH  - Sirtuin 1/*metabolism
MH  - TOR Serine-Threonine Kinases/metabolism
MH  - Tumor Suppressor Protein p53/metabolism
PMC - PMC8273810
OTO - NOTNLM
OT  - autophagy
OT  - ionizing radiation
OT  - radiation protection
OT  - resveratrol
OT  - sirtuin 1 (SIRT1)
EDAT- 2021/04/30 06:00
MHDA- 2021/12/24 06:00
PMCR- 2021/04/29
CRDT- 2021/04/29 06:37
PHST- 2020/09/22 00:00 [received]
PHST- 2021/01/02 00:00 [revised]
PHST- 2021/04/30 06:00 [pubmed]
PHST- 2021/12/24 06:00 [medline]
PHST- 2021/04/29 06:37 [entrez]
PHST- 2021/04/29 00:00 [pmc-release]
AID - 6257739 [pii]
AID - rrab035 [pii]
AID - 10.1093/jrr/rrab035 [doi]
PST - ppublish
SO  - J Radiat Res. 2021 Jul 10;62(4):574-581. doi: 10.1093/jrr/rrab035.