PMID- 33914756
OWN - NLM
STAT- MEDLINE
DCOM- 20211012
LR  - 20211012
IS  - 1932-6203 (Electronic)
IS  - 1932-6203 (Linking)
VI  - 16
IP  - 4
DP  - 2021
TI  - TGFbeta1, MMPs and cytokines profiles in ocular surface: Possible tear biomarkers 
      for pseudoexfoliation.
PG  - e0249759
LID - 10.1371/journal.pone.0249759 [doi]
LID - e0249759
AB  - PURPOSE: Pseudoexfoliation (PXF) is a unique form of glaucoma characterized by 
      accumulation of exfoliative material in the eyes. Changes in tear profile in 
      disease stages may give us insights into molecular mechanisms involved in causing 
      glaucoma in the eye. METHODS: All patients were categorized into three main 
      categories; pseudoexfoliation (PXF), pseudoexfoliation glaucoma (PXG) and 
      cataract, which served as control. Cytokines, transforming growth factor beta1 
      (TGFbeta1), matrix metalloproteases (MMPs) and fibronectin (FN1) were assessed with 
      multiplex bead assay, enzyme-linked immunosorbent assay (ELISA), gelatin 
      zymography, and immunohistochemistry (IHC) respectively in different ocular 
      tissues such as tears, tenon's capsule, aqueous humor (AH) and serum samples of 
      patients with PXF stages. RESULTS: We found that TGFbeta1, MMP-9 and FN1 protein 
      expression were upregulated in tears, tenon's capsule and AH samples in PXG 
      compared to PXF, though the MMP-9 protein activity was downregulated in PXG 
      compared with control or PXF. We have also found that in PXG tears sample the 
      fold change of TGF-alpha (Transforming Growth Factor-alpha), MDC (Macrophage Derived 
      Chemokine), IL-8 (Interleukin-8), VEGF (Vascular Endothelial Growth Factor) were 
      significantly downregulated and the levels of GM-CSF (Granulocyte Macrophage 
      Colony Stimulating Factor), IP-10 (Interferon- gamma produced protein-10) were 
      significant upregulated. While in AH; IL-6 (Interleukin-6), IL-8, VEGF, IFN-a2 
      (Interferon- alpha2), GRO (Growth regulated alpha protein) levels were found lower 
      and IL1a (Interleukin-1alpha) level was higher in PXG compared to PXF. And in serum; 
      IFN-a2, Eotaxin, GM-CSF, Fractalkine, IL-10 (Interleukin-10), IL1Ra 
      (Interleukin-1 receptor antagonist), IL-7 (Interleukin-7), IL-8, MIP1beta 
      (Macrophage Inflammatory Protein-1beta), MCP-1 (Monocyte Chemoattractant Protein-1) 
      levels were significantly upregulated and PDGF-AA (Platelet Derived Growth 
      Factor-AA) level was downregulated in the patients with PXG compared to PXF. 
      CONCLUSIONS: Altered expression of these molecules in tears may therefore be used 
      as a signal for onset of glaucoma or for identifying eyes at risk of developing 
      glaucoma in PXF.
FAU - Sahay, Prity
AU  - Sahay P
AD  - Hyderabad Eye Research Foundation (HERF), L.V. Prasad Eye Institute, Patia, 
      Bhubaneswar, Odisha, India.
AD  - KIIT School of Biotechnology, Patia, Bhubaneswar, Odisha, India.
FAU - Reddy, Shweta
AU  - Reddy S
AD  - Hyderabad Eye Research Foundation (HERF), L.V. Prasad Eye Institute, Patia, 
      Bhubaneswar, Odisha, India.
FAU - Prusty, Birendra Kumar
AU  - Prusty BK
AD  - Institute of Life Science, Bhubaneswar, Odisha, India.
FAU - Modak, Rahul
AU  - Modak R
AD  - KIIT School of Biotechnology, Patia, Bhubaneswar, Odisha, India.
FAU - Rao, Aparna
AU  - Rao A
AUID- ORCID: 0000-0001-8120-8740
AD  - Hyderabad Eye Research Foundation (HERF), L.V. Prasad Eye Institute, Patia, 
      Bhubaneswar, Odisha, India.
AD  - KIIT School of Biotechnology, Patia, Bhubaneswar, Odisha, India.
LA  - eng
GR  - WT_/Wellcome Trust/United Kingdom
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20210429
PL  - United States
TA  - PLoS One
JT  - PloS one
JID - 101285081
RN  - 0 (Biomarkers)
RN  - 0 (Cytokines)
RN  - 0 (TGFB1 protein, human)
RN  - 0 (Transforming Growth Factor beta1)
RN  - EC 3.4.24.- (Matrix Metalloproteinases)
SB  - IM
MH  - Biomarkers/*metabolism
MH  - Cytokines/*metabolism
MH  - Exfoliation Syndrome/diagnosis/*metabolism/pathology
MH  - Eye/*metabolism/pathology
MH  - Female
MH  - Humans
MH  - Male
MH  - Matrix Metalloproteinases/*metabolism
MH  - Middle Aged
MH  - Tears/*metabolism
MH  - Transforming Growth Factor beta1/*metabolism
PMC - PMC8084233
COIS- The authors have declared that no competing interests exist.
EDAT- 2021/04/30 06:00
MHDA- 2021/10/13 06:00
PMCR- 2021/04/29
CRDT- 2021/04/29 17:31
PHST- 2021/01/12 00:00 [received]
PHST- 2021/03/24 00:00 [accepted]
PHST- 2021/04/29 17:31 [entrez]
PHST- 2021/04/30 06:00 [pubmed]
PHST- 2021/10/13 06:00 [medline]
PHST- 2021/04/29 00:00 [pmc-release]
AID - PONE-D-21-01128 [pii]
AID - 10.1371/journal.pone.0249759 [doi]
PST - epublish
SO  - PLoS One. 2021 Apr 29;16(4):e0249759. doi: 10.1371/journal.pone.0249759. 
      eCollection 2021.