PMID- 33914996
OWN - NLM
STAT- MEDLINE
DCOM- 20230207
LR  - 20230207
IS  - 1601-0825 (Electronic)
IS  - 1354-523X (Linking)
VI  - 29
IP  - 2
DP  - 2023 Mar
TI  - SLCO4A1-AS1 regulates laryngeal squamous cell carcinoma cell phenotypes via the 
      Wnt pathway.
PG  - 390-401
LID - 10.1111/odi.13893 [doi]
AB  - AIM: Long non-coding RNAs were widely reported to regulate laryngeal squamous 
      cell carcinoma (LSCC), a prevalent tumor in the head and neck. We aimed to 
      investigate the role of solute carrier organic anion transporter family member 
      4A1 antisense RNA 1 (SLCO4A1-AS1) in LSCC. MATERIALS & METHODS: CCK-8 and colony 
      formation assays were conducted to examine the viability and proliferation of 
      LSCC cells. The apoptosis of LSCC cells was evaluated using flow cytometry and 
      TUNEL assays. The distribution of SLCO4A1-AS1 in LSCC cells was detected by 
      subcellular fractionation assay. The interaction between molecules was confirmed 
      using luciferase reporter assay. RESULTS: SLCO4A1-AS1 was overexpressed in LSCC 
      tissues and cells. Furthermore, silenced SLCO4A1-AS1 repressed the proliferation 
      and facilitated apoptosis of LSCC cells. Mechanistical investigation revealed 
      that SLCO4A1-AS1 was a competing endogenous RNA (ceRNA) to upregulate SETD7 by 
      binding with miR-7855-p. Additionally, SLCO4A1-AS1 positively regulated the 
      Wnt/beta-catenin signaling pathway by upregulating SETD7. Rescue experiments 
      demonstrated that SLCO4A1-AS1 promoted LSCC proliferation and inhibited LSCC 
      apoptosis by upregulation of SETD7 and activation of the Wnt/beta-catenin pathway. 
      CONCLUSION: SLCO4A1-AS1 promotes proliferation and inhibits apoptosis of LSCC 
      cells by upregulation of SETD7 in a miR-7855-5p dependent way to activate the 
      Wnt/beta-catenin pathway.
CI  - (c) 2021 Wiley Periodicals LLC.
FAU - Zhang, Feng
AU  - Zhang F
AD  - Department of Otolaryngology, Yongchuan Hospital (T.C.M) of Chongqing Medical 
      University, Chongqing, China.
FAU - Mao, Dehong
AU  - Mao D
AD  - Department of Otolaryngology, Yongchuan Hospital (T.C.M) of Chongqing Medical 
      University, Chongqing, China.
FAU - He, Zhongmei
AU  - He Z
AUID- ORCID: 0000-0002-4488-8297
AD  - Department of Otolaryngology, Yongchuan Hospital (T.C.M) of Chongqing Medical 
      University, Chongqing, China.
FAU - Li, Weichun
AU  - Li W
AD  - Department of Otolaryngology, Yongchuan Hospital (T.C.M) of Chongqing Medical 
      University, Chongqing, China.
FAU - Zhang, Xu
AU  - Zhang X
AD  - Department of Otolaryngology, Yongchuan Hospital (T.C.M) of Chongqing Medical 
      University, Chongqing, China.
FAU - Li, Linglong
AU  - Li L
AD  - Department of Otolaryngology, Yongchuan Hospital (T.C.M) of Chongqing Medical 
      University, Chongqing, China.
LA  - eng
PT  - Journal Article
DEP - 20210511
PL  - Denmark
TA  - Oral Dis
JT  - Oral diseases
JID - 9508565
RN  - 0 (MicroRNAs)
RN  - 0 (beta Catenin)
RN  - 0 (RNA, Long Noncoding)
RN  - EC 2.1.1.43 (SETD7 protein, human)
RN  - EC 2.1.1.43 (Histone-Lysine N-Methyltransferase)
MH  - Humans
MH  - Squamous Cell Carcinoma of Head and Neck/genetics
MH  - Wnt Signaling Pathway/genetics
MH  - *MicroRNAs/genetics/metabolism
MH  - beta Catenin/metabolism
MH  - Cell Line, Tumor
MH  - Cell Proliferation/genetics
MH  - *Head and Neck Neoplasms/genetics
MH  - *RNA, Long Noncoding/genetics
MH  - Gene Expression Regulation, Neoplastic
MH  - Histone-Lysine N-Methyltransferase/genetics/metabolism
OTO - NOTNLM
OT  - LSCC
OT  - SETD7
OT  - SLCO4A1-AS1
OT  - miR-7855-5p
EDAT- 2021/04/30 06:00
MHDA- 2023/02/08 06:00
CRDT- 2021/04/29 17:43
PHST- 2021/04/14 00:00 [revised]
PHST- 2020/12/02 00:00 [received]
PHST- 2021/04/19 00:00 [accepted]
PHST- 2021/04/30 06:00 [pubmed]
PHST- 2023/02/08 06:00 [medline]
PHST- 2021/04/29 17:43 [entrez]
AID - 10.1111/odi.13893 [doi]
PST - ppublish
SO  - Oral Dis. 2023 Mar;29(2):390-401. doi: 10.1111/odi.13893. Epub 2021 May 11.