PMID- 33917526
OWN - NLM
STAT- MEDLINE
DCOM- 20210512
LR  - 20240331
IS  - 1422-0067 (Electronic)
IS  - 1422-0067 (Linking)
VI  - 22
IP  - 7
DP  - 2021 Apr 6
TI  - Argon Plasma Exposure Augments Costimulatory Ligands and Cytokine Release in 
      Human Monocyte-Derived Dendritic Cells.
LID - 10.3390/ijms22073790 [doi]
LID - 3790
AB  - Cold physical plasma is a partially ionized gas expelling many reactive oxygen 
      and nitrogen species (ROS/RNS). Several plasma devices have been licensed for 
      medical use in dermatology, and recent experimental studies suggest their 
      putative role in cancer treatment. In cancer therapies with an immunological 
      dimension, successful antigen presentation and inflammation modulation is a key 
      hallmark to elicit antitumor immunity. Dendritic cells (DCs) are critical for 
      this task. However, the inflammatory consequences of DCs following plasma 
      exposure are unknown. To this end, human monocyte-derived DCs (moDCs) were 
      expanded from isolated human primary monocytes; exposed to plasma; and their 
      metabolic activity, surface marker expression, and cytokine profiles were 
      analyzed. As controls, hydrogen peroxide, hypochlorous acid, and peroxynitrite 
      were used. Among all types of ROS/RNS-mediated treatments, plasma exposure 
      exerted the most notable increase of activation markers at 24 h such as CD25, 
      CD40, and CD83 known to be crucial for T cell costimulation. Moreover, the 
      treatments increased interleukin (IL)-1alpha, IL-6, and IL-23. Altogether, this study 
      suggests plasma treatment augmenting costimulatory ligand and cytokine expression 
      in human moDCs, which might exert beneficial effects in the tumor 
      microenvironment.
FAU - Bekeschus, Sander
AU  - Bekeschus S
AUID- ORCID: 0000-0002-8773-8862
AD  - The Centre for Innovation Competence (ZIK) Plasmatis, Leibniz Institute for 
      Plasma Science and Technology (INP), 17489 Greifswald, Germany.
FAU - Meyer, Dorothee
AU  - Meyer D
AD  - The Centre for Innovation Competence (ZIK) Plasmatis, Leibniz Institute for 
      Plasma Science and Technology (INP), 17489 Greifswald, Germany.
FAU - Arlt, Kevin
AU  - Arlt K
AD  - The Centre for Innovation Competence (ZIK) Plasmatis, Leibniz Institute for 
      Plasma Science and Technology (INP), 17489 Greifswald, Germany.
FAU - von Woedtke, Thomas
AU  - von Woedtke T
AUID- ORCID: 0000-0002-1097-4832
AD  - The Centre for Innovation Competence (ZIK) Plasmatis, Leibniz Institute for 
      Plasma Science and Technology (INP), 17489 Greifswald, Germany.
AD  - Institute of Hygiene and Environmental Medicine, Greifswald University Medical 
      Center, 17475 Greifswald, Germany.
FAU - Miebach, Lea
AU  - Miebach L
AD  - The Centre for Innovation Competence (ZIK) Plasmatis, Leibniz Institute for 
      Plasma Science and Technology (INP), 17489 Greifswald, Germany.
AD  - Department of General, Visceral, Thoracic, and Vascular Surgery, Greifswald 
      University Medical Center, 17475 Greifswald, Germany.
FAU - Freund, Eric
AU  - Freund E
AUID- ORCID: 0000-0003-2950-0984
AD  - The Centre for Innovation Competence (ZIK) Plasmatis, Leibniz Institute for 
      Plasma Science and Technology (INP), 17489 Greifswald, Germany.
AD  - Department of General, Visceral, Thoracic, and Vascular Surgery, Greifswald 
      University Medical Center, 17475 Greifswald, Germany.
FAU - Clemen, Ramona
AU  - Clemen R
AD  - The Centre for Innovation Competence (ZIK) Plasmatis, Leibniz Institute for 
      Plasma Science and Technology (INP), 17489 Greifswald, Germany.
LA  - eng
GR  - 03Z22DN11/Bundesministerium fur Bildung und Forschung/
GR  - 03Z22Di1/Bundesministerium fur Bildung und Forschung/
PT  - Journal Article
DEP - 20210406
PL  - Switzerland
TA  - Int J Mol Sci
JT  - International journal of molecular sciences
JID - 101092791
RN  - 0 (Antigens, CD)
RN  - 0 (Cytokines)
RN  - 0 (Ligands)
RN  - 0 (Plasma Gases)
RN  - 67XQY1V3KH (Argon)
SB  - IM
MH  - Antigens, CD/*metabolism
MH  - Argon/*pharmacology
MH  - Cytokines/*metabolism
MH  - Dendritic Cells/*metabolism
MH  - Humans
MH  - Ligands
MH  - Monocytes/*metabolism
MH  - Plasma Gases/*pharmacology
MH  - Tumor Microenvironment/drug effects
PMC - PMC8038845
OTO - NOTNLM
OT  - CAP
OT  - RNS
OT  - ROS
OT  - cancer
OT  - cold atmospheric pressure plasma
OT  - hydrogen peroxide
OT  - hypochlorous acid
OT  - moDCs
OT  - peroxynitrite
OT  - reactive oxygen and nitrogen species
COIS- The authors declare no conflict of interest. The founding sponsors had no role in 
      the design of the study; in the collection, analyses, or interpretation of data; 
      in the writing of the manuscript; or in the decision to publish the results.
EDAT- 2021/05/01 06:00
MHDA- 2021/05/13 06:00
PMCR- 2021/04/06
CRDT- 2021/04/30 01:06
PHST- 2021/03/21 00:00 [received]
PHST- 2021/03/29 00:00 [revised]
PHST- 2021/03/30 00:00 [accepted]
PHST- 2021/04/30 01:06 [entrez]
PHST- 2021/05/01 06:00 [pubmed]
PHST- 2021/05/13 06:00 [medline]
PHST- 2021/04/06 00:00 [pmc-release]
AID - ijms22073790 [pii]
AID - ijms-22-03790 [pii]
AID - 10.3390/ijms22073790 [doi]
PST - epublish
SO  - Int J Mol Sci. 2021 Apr 6;22(7):3790. doi: 10.3390/ijms22073790.