PMID- 33917956
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20210502
IS  - 2075-4426 (Print)
IS  - 2075-4426 (Electronic)
IS  - 2075-4426 (Linking)
VI  - 11
IP  - 4
DP  - 2021 Apr 8
TI  - The Effects of Dimethylsulfoxide and Oxygen on DNA Damage Induction and Repair 
      Outcomes for Cells Irradiated by 62 MeV Proton and 3.31 MeV Helium Ions.
LID - 10.3390/jpm11040286 [doi]
LID - 286
AB  - Reactive oxygen species (ROS) play an essential role in radiation-induced 
      indirect actions. In terms of DNA damage, double strand breaks (DSBs) have the 
      greatest effects on the repair of DNA damage, cell survival and transformation. 
      This study evaluated the biological effects of the presence of ROS and oxygen on 
      DSB induction and mutation frequency. The relative biological effectiveness (RBE) 
      and oxygen enhancement ratio (OER) of 62 MeV therapeutic proton beams and 3.31 
      MeV helium ions were calculated using Monte Carlo damage simulation (MCDS) 
      software. Monte Carlo excision repair (MCER) simulations were used to calculate 
      the repair outcomes (mutation frequency). The RBE values of proton beams 
      decreased to 0.75 in the presence of 0.4 M dimethylsulfoxide (DMSO) and then 
      increases to 0.9 in the presence of 2 M DMSO while the RBE values of 3.31 MeV 
      helium ions increased from 2.9 to 5.7 (0-2 M). The mutation frequency of proton 
      beams also decreased from 0.008-0.065 to 0.004-0.034 per cell per Gy by the 
      addition of 2 M DMSO, indicating that ROS affects both DSB induction and repair 
      outcomes. These results show that the combined use of DMSO in normal tissues and 
      an increased dose in tumor regions increases treatment efficiency.
FAU - Chan, Chun-Chieh
AU  - Chan CC
AD  - Department of Electrical Engineering, National Chung Hsing University, Taichung 
      40227, Taiwan.
FAU - Hsiao, Ya-Yun
AU  - Hsiao YY
AUID- ORCID: 0000-0001-7494-8767
AD  - Department of Radiology, Chung Shan Medical University Hospital, Taichung 40201, 
      Taiwan.
AD  - Department of Medical Imaging and Radiological Sciences, Chung Shan Medical 
      University, Taichung 40201, Taiwan.
LA  - eng
PT  - Journal Article
DEP - 20210408
PL  - Switzerland
TA  - J Pers Med
JT  - Journal of personalized medicine
JID - 101602269
PMC - PMC8068342
OTO - NOTNLM
OT  - DNA damage induction
OT  - dimethylsulfoxide
OT  - hypoxia
OT  - reactive oxygen species
COIS- The authors declare no conflict of interest.
EDAT- 2021/05/01 06:00
MHDA- 2021/05/01 06:01
PMCR- 2021/04/08
CRDT- 2021/04/30 01:07
PHST- 2021/03/19 00:00 [received]
PHST- 2021/04/06 00:00 [accepted]
PHST- 2021/04/30 01:07 [entrez]
PHST- 2021/05/01 06:00 [pubmed]
PHST- 2021/05/01 06:01 [medline]
PHST- 2021/04/08 00:00 [pmc-release]
AID - jpm11040286 [pii]
AID - jpm-11-00286 [pii]
AID - 10.3390/jpm11040286 [doi]
PST - epublish
SO  - J Pers Med. 2021 Apr 8;11(4):286. doi: 10.3390/jpm11040286.