PMID- 33919402
OWN - NLM
STAT- MEDLINE
DCOM- 20210823
LR  - 20231111
IS  - 2073-4425 (Electronic)
IS  - 2073-4425 (Linking)
VI  - 12
IP  - 5
DP  - 2021 Apr 21
TI  - Comparative Distribution of Repetitive Sequences in the Karyotypes of Xenopus 
      tropicalis and Xenopus laevis (Anura, Pipidae).
LID - 10.3390/genes12050617 [doi]
LID - 617
AB  - Xenopus laevis and its diploid relative, Xenopus tropicalis, are the most used 
      amphibian models. Their genomes have been sequenced, and they are emerging as 
      model organisms for research into disease mechanisms. Despite the growing 
      knowledge on their genomes based on data obtained from massive genome sequencing, 
      basic research on repetitive sequences in these species is lacking. This study 
      conducted a comparative analysis of repetitive sequences in X. laevis and X. 
      tropicalis. Genomic in situ hybridization (GISH) and fluorescence in situ 
      hybridization (FISH) with Cot DNA of both species revealed a conserved enrichment 
      of repetitive sequences at the ends of the chromosomes in these Xenopus species. 
      The repeated sequences located on the short arm of chromosome 3 from X. 
      tropicalis were not related to the sequences on the short arm of chromosomes 3L 
      and 3S from X. laevis, although these chromosomes were homoeologous, indicating 
      that these regions evolved independently in these species. Furthermore, all the 
      other repetitive sequences in X. tropicalis and X. laevis may be 
      species-specific, as they were not revealed in cross-species hybridizations. 
      Painting experiments in X. laevis with chromosome 7 from X. tropicalis revealed 
      shared sequences with the short arm of chromosome 3L. These regions could be 
      related by the presence of the nucleolus organizer region (NOR) in both 
      chromosomes, although the region revealed by chromosome painting in the short arm 
      of chromosome 3L in X. laevis did not correspond to 18S + 28S rDNA sequences, as 
      they did not colocalize. The identification of these repeated sequences is of 
      interest as they provide an explanation to some problems already described in the 
      genome assemblies of these species. Furthermore, the distribution of repetitive 
      DNA in the genomes of X. laevis and X. tropicalis might be a valuable marker to 
      assist us in understanding the genome evolution in a group characterized by 
      numerous polyploidization events coupled with hybridizations.
FAU - Roco, Alvaro S
AU  - Roco AS
AUID- ORCID: 0000-0002-7363-3939
AD  - Department of Experimental Biology, Faculty of Experimental Sciences, University 
      of Jaen, Campus Las Lagunillas S/N, 23071 Jaen, Spain.
FAU - Liehr, Thomas
AU  - Liehr T
AUID- ORCID: 0000-0003-1672-3054
AD  - Institute of Human Genetics, Jena University Hospital, Friedrich Schiller 
      University, Am Klinikum 1, D-07747 Jena, Germany.
FAU - Ruiz-Garcia, Adrian
AU  - Ruiz-Garcia A
AUID- ORCID: 0000-0002-6773-5066
AD  - Department of Experimental Biology, Faculty of Experimental Sciences, University 
      of Jaen, Campus Las Lagunillas S/N, 23071 Jaen, Spain.
FAU - Guzman, Kateryna
AU  - Guzman K
AD  - Department of Experimental Biology, Faculty of Experimental Sciences, University 
      of Jaen, Campus Las Lagunillas S/N, 23071 Jaen, Spain.
FAU - Bullejos, Monica
AU  - Bullejos M
AUID- ORCID: 0000-0003-3256-8840
AD  - Department of Experimental Biology, Faculty of Experimental Sciences, University 
      of Jaen, Campus Las Lagunillas S/N, 23071 Jaen, Spain.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20210421
PL  - Switzerland
TA  - Genes (Basel)
JT  - Genes
JID - 101551097
SB  - IM
MH  - Animals
MH  - Chromosomes/genetics
MH  - Genetic Speciation
MH  - In Situ Hybridization, Fluorescence
MH  - *Karyotype
MH  - *Repetitive Sequences, Nucleic Acid
MH  - Xenopus/classification/*genetics
PMC - PMC8143290
OTO - NOTNLM
OT  - Cot DNA
OT  - Xenopus laevis
OT  - Xenopus tropicalis
OT  - chromosome painting
OT  - fluorescence in situ hybridization (FISH)
OT  - genomic in situ hybridization (GISH)
OT  - repetitive DNA
COIS- The authors declare no conflict of interest.
EDAT- 2021/05/01 06:00
MHDA- 2021/08/24 06:00
PMCR- 2021/04/21
CRDT- 2021/04/30 01:12
PHST- 2021/03/30 00:00 [received]
PHST- 2021/04/14 00:00 [revised]
PHST- 2021/04/19 00:00 [accepted]
PHST- 2021/04/30 01:12 [entrez]
PHST- 2021/05/01 06:00 [pubmed]
PHST- 2021/08/24 06:00 [medline]
PHST- 2021/04/21 00:00 [pmc-release]
AID - genes12050617 [pii]
AID - genes-12-00617 [pii]
AID - 10.3390/genes12050617 [doi]
PST - epublish
SO  - Genes (Basel). 2021 Apr 21;12(5):617. doi: 10.3390/genes12050617.