PMID- 33920315
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20210518
IS  - 2076-2615 (Print)
IS  - 2076-2615 (Electronic)
IS  - 2076-2615 (Linking)
VI  - 11
IP  - 4
DP  - 2021 Apr 10
TI  - Melatonin Restores the Developmental Competence of Heat Stressed Porcine Oocytes, 
      and Alters the Expression of Genes Related to Oocyte Maturation.
LID - 10.3390/ani11041086 [doi]
LID - 1086
AB  - Melatonin enhances the quality and in vitro maturation (IVM) of oocytes under 
      heat stress (HS), but the mechanism of melatonin in reducing HS injury on oocytes 
      is not fully understood. In this study, porcine cumulus-oocyte complexes (COCs) 
      were randomly divided into three groups. The COCs of the control group were 
      cultured at 38.5  degrees C for 42 h, and the COCs of the HS group were cultured at 41.5 
       degrees C for 4 h, and then transferred into 38.5  degrees C for 38 h. The COCs of the HS + 
      melatonin group were cultured with 10(-9) M melatonin under the same conditions 
      as the HS group. The survival rate, maturation rate, distribution of alpha-tubulin 
      and F-actin of the oocytes were assessed. In addition, the expression profiles 
      for genes related to the oocyte maturation, including heat shock protein 70 
      (HSP70), nuclear factor erythroid 2-related factor 2 (NRF2), cyclin-dependent 
      kinase 1 (CDK1), growth differentiation factor 9 (GDF9) were analyzed by 
      real-time quantitative PCR. The results showed that HS decreased the survival 
      rate and maturation rate, distribution of alpha-tubulin and F-actin, but melatonin 
      treatment could partly counteract these adverse effects. In addition, HS 
      increased expression of HSP70 and NRF2 mRNA, and melatonin treatment had a 
      similar effect on HSP70 expression, but had a contrary effect on NRF2 expression. 
      Furthermore, HS inhibited expression of CDK1 and GDF9 mRNA, but melatonin 
      treatment could weaken the effect on GDF9 expression induced by HS. In summary, 
      melatonin treatment could attenuate the unfavorable effects induced by HS to 
      enhance developmental competence of porcine oocytes during IVM.
FAU - Yang, Ling
AU  - Yang L
AUID- ORCID: 0000-0003-4385-0024
AD  - School of Life Sciences and Food Engineering, Hebei University of Engineering, 
      Handan 056038, China.
FAU - Zhao, Zimo
AU  - Zhao Z
AUID- ORCID: 0000-0001-9552-0997
AD  - School of Life Sciences and Food Engineering, Hebei University of Engineering, 
      Handan 056038, China.
AD  - Animal Husbandry and Veterinary Research Institute of Tianjin, Tianjin 300412, 
      China.
FAU - Cui, Maosheng
AU  - Cui M
AD  - Animal Husbandry and Veterinary Research Institute of Tianjin, Tianjin 300412, 
      China.
FAU - Zhang, Leying
AU  - Zhang L
AD  - School of Life Sciences and Food Engineering, Hebei University of Engineering, 
      Handan 056038, China.
FAU - Li, Qianjun
AU  - Li Q
AD  - Animal Husbandry and Veterinary Research Institute of Tianjin, Tianjin 300412, 
      China.
LA  - eng
GR  - 2016YFD0500502/National Key Research and Development Program of China/
GR  - ZD2016069/Technology Research Project of Higher Education Institutions of Hebei 
      Province, China/
PT  - Journal Article
DEP - 20210410
PL  - Switzerland
TA  - Animals (Basel)
JT  - Animals : an open access journal from MDPI
JID - 101635614
PMC - PMC8069948
OTO - NOTNLM
OT  - in vitro maturation
OT  - melatonin
OT  - oocyte
OT  - pig
COIS- The authors declare no conflict of interest.
EDAT- 2021/05/01 06:00
MHDA- 2021/05/01 06:01
PMCR- 2021/04/10
CRDT- 2021/04/30 01:15
PHST- 2021/03/12 00:00 [received]
PHST- 2021/04/06 00:00 [revised]
PHST- 2021/04/08 00:00 [accepted]
PHST- 2021/04/30 01:15 [entrez]
PHST- 2021/05/01 06:00 [pubmed]
PHST- 2021/05/01 06:01 [medline]
PHST- 2021/04/10 00:00 [pmc-release]
AID - ani11041086 [pii]
AID - animals-11-01086 [pii]
AID - 10.3390/ani11041086 [doi]
PST - epublish
SO  - Animals (Basel). 2021 Apr 10;11(4):1086. doi: 10.3390/ani11041086.