PMID- 33920430
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20210502
IS  - 2076-2615 (Print)
IS  - 2076-2615 (Electronic)
IS  - 2076-2615 (Linking)
VI  - 11
IP  - 4
DP  - 2021 Apr 11
TI  - mRNA Expressions of Candidate Genes in Gestational Day 16 Conceptus and 
      Corresponding Endometrium in Repeat Breeder Dairy Cows with Suboptimal Uterine 
      Environment Following Transfer of Different Quality Day 7 Embryos.
LID - 10.3390/ani11041092 [doi]
LID - 1092
AB  - Effect of the gestational day (GD) 7 embryo quality grade (QG) and subclinical 
      endometritis (SCE) on mRNA and protein expressions of candidate genes 
      [Interferon-tau (IFNT), IFN stimulated genes (ISG15, CTSL1, RSAD2, SLC2A1, CXCL10, 
      and SLC27A6), Peroxisome proliferator activated receptors (PPARA, D, and G), 
      Retinoid X receptors (RXRA, B, and G), and Mucin-1 (MUC1)] in GD16 conceptus and 
      corresponding endometrium were evaluated. After screening of performance records 
      (n = 2389) and selection of repeat breeders (n = 681), cows with SCE (>/=6% 
      polymorphonuclear neutrophils-PMN; n = 180) and no-SCE (<6%PMN; n = 180) received 
      GD7 embryos of different QGs. Based on GD16 conceptus recovery, cows with SCE (n 
      = 30) and No- SCE (n = 30) that received GD7 embryos QG1 (good, n = 20), 2 (fair, 
      n = 20), and 3 (poor, n = 20) were included for gene analysis. mRNA and protein 
      expressions (IFNT, ISG15, CXCL10, PPARG, RXRG, SLC2A1, and SLC27A6) differed 
      between SCE and embryo QG groups. All genes but MUC1 and all proteins but MUC1 
      expression was greater in filamentous conceptus and corresponding endometrium vs. 
      tubular conceptus and matching endometrium in SCE and embryo QG groups. In 
      conclusion, disrupted embryo-uterine communication by altered expression of 
      candidate genes in SCE cows, and in cows following the transfer of poor embryo 
      negatively programs the conceptus development and plausibly affects conceptus 
      survival.
FAU - Kasimanickam, Ramanathan K
AU  - Kasimanickam RK
AUID- ORCID: 0000-0003-1117-7867
AD  - Department of Veterinary Clinical Sciences, College of Veterinary Medicine, 
      Washington State University, Pullman, WA 99164, USA.
FAU - Kasimanickam, Vanmathy R
AU  - Kasimanickam VR
AD  - Department of Veterinary Clinical Sciences, College of Veterinary Medicine, 
      Washington State University, Pullman, WA 99164, USA.
AD  - AARVEE Animal Biotech LLC, Corvallis, OR 97333, USA.
LA  - eng
PT  - Journal Article
DEP - 20210411
PL  - Switzerland
TA  - Animals (Basel)
JT  - Animals : an open access journal from MDPI
JID - 101635614
PMC - PMC8070175
OTO - NOTNLM
OT  - conceptus elongation
OT  - dairy cows
OT  - embryo quality
OT  - endometrial inflammation
OT  - mRNA expression
OT  - protein
COIS- The authors declare no conflict of interest.
EDAT- 2021/05/01 06:00
MHDA- 2021/05/01 06:01
PMCR- 2021/04/11
CRDT- 2021/04/30 01:15
PHST- 2021/03/13 00:00 [received]
PHST- 2021/04/01 00:00 [revised]
PHST- 2021/04/06 00:00 [accepted]
PHST- 2021/04/30 01:15 [entrez]
PHST- 2021/05/01 06:00 [pubmed]
PHST- 2021/05/01 06:01 [medline]
PHST- 2021/04/11 00:00 [pmc-release]
AID - ani11041092 [pii]
AID - animals-11-01092 [pii]
AID - 10.3390/ani11041092 [doi]
PST - epublish
SO  - Animals (Basel). 2021 Apr 11;11(4):1092. doi: 10.3390/ani11041092.