PMID- 33923285
OWN - NLM
STAT- MEDLINE
DCOM- 20210514
LR  - 20210518
IS  - 1422-0067 (Electronic)
IS  - 1422-0067 (Linking)
VI  - 22
IP  - 9
DP  - 2021 Apr 24
TI  - Prolactin-Releasing Peptide Differentially Regulates Gene Transcriptomic Profiles 
      in Mouse Bone Marrow-Derived Macrophages.
LID - 10.3390/ijms22094456 [doi]
LID - 4456
AB  - Prolactin-releasing Peptide (PrRP) is a neuropeptide whose receptor is GPR10. 
      Recently, the regulatory role of PrRP in the neuroendocrine field has attracted 
      increasing attention. However, the influence of PrRP on macrophages, the critical 
      housekeeper in the neuroendocrine field, has not yet been fully elucidated. Here, 
      we investigated the effect of PrRP on the transcriptome of mouse bone 
      marrow-derived macrophages (BMDMs) with RNA sequencing, bioinformatics, and 
      molecular simulation. BMDMs were exposed to PrRP (18 h) and were subjected to RNA 
      sequencing. Differentially expressed genes (DEGs) were acquired, followed by GO, 
      KEGG, and PPI analysis. Eight qPCR-validated DEGs were chosen as hub genes. Next, 
      the three-dimensional structures of the proteins encoded by these hub genes were 
      modeled by Rosetta and Modeller, followed by molecular dynamics simulation by the 
      Gromacs program. Finally, the binding modes between PrRP and hub proteins were 
      investigated with the Rosetta program. PrRP showed no noticeable effect on the 
      morphology of macrophages. A total of 410 DEGs were acquired, and PrRP regulated 
      multiple BMDM-mediated functional pathways. Besides, the possible docking modes 
      between PrRP and hub proteins were investigated. Moreover, GPR10 was expressed on 
      the cell membrane of BMDMs, which increased after PrRP exposure. Collectively, 
      PrRP significantly changed the transcriptome profile of BMDMs, implying that PrRP 
      may be involved in various physiological activities mastered by macrophages.
FAU - Sun, Yulong
AU  - Sun Y
AUID- ORCID: 0000-0002-6811-5926
AD  - School of Life Sciences, Northwestern Polytechnical University, Xi'an 710072, 
      China.
AD  - Key Laboratory for Space Biosciences & Biotechnology, Institute of Special 
      Environmental Biophysics, School of Life Sciences, Northwestern Polytechnical 
      University, Xi'an 710072, China.
FAU - Zuo, Zhuo
AU  - Zuo Z
AD  - School of Life Sciences, Northwestern Polytechnical University, Xi'an 710072, 
      China.
AD  - Key Laboratory for Space Biosciences & Biotechnology, Institute of Special 
      Environmental Biophysics, School of Life Sciences, Northwestern Polytechnical 
      University, Xi'an 710072, China.
FAU - Kuang, Yuanyuan
AU  - Kuang Y
AD  - School of Life Sciences, Northwestern Polytechnical University, Xi'an 710072, 
      China.
AD  - Key Laboratory for Space Biosciences & Biotechnology, Institute of Special 
      Environmental Biophysics, School of Life Sciences, Northwestern Polytechnical 
      University, Xi'an 710072, China.
LA  - eng
GR  - 2020JM-148/Natural Science Basic Research Program of Shaanxi Province/
GR  - 2018BSHYDZZ48/Shaanxi Province Postdoctoral Science Foundation/
GR  - 2017M623250/China Postdoctoral Science Foundation/
PT  - Journal Article
DEP - 20210424
PL  - Switzerland
TA  - Int J Mol Sci
JT  - International journal of molecular sciences
JID - 101092791
RN  - 0 (Prolactin-Releasing Hormone)
SB  - IM
MH  - Animals
MH  - Computational Biology
MH  - Gene Expression Regulation/*drug effects
MH  - Macrophages/drug effects/*metabolism
MH  - Male
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Prolactin-Releasing Hormone/*pharmacology
MH  - Transcriptome/*drug effects
PMC - PMC8123224
OTO - NOTNLM
OT  - GPR10
OT  - RNA sequencing
OT  - bioinformatics
OT  - bone marrow-derived macrophage
OT  - prolactin-releasing peptide
OT  - transcriptomic profiles
COIS- The authors declare no conflict of interest.
EDAT- 2021/05/01 06:00
MHDA- 2021/05/15 06:00
PMCR- 2021/04/24
CRDT- 2021/04/30 01:24
PHST- 2021/03/02 00:00 [received]
PHST- 2021/04/14 00:00 [revised]
PHST- 2021/04/20 00:00 [accepted]
PHST- 2021/04/30 01:24 [entrez]
PHST- 2021/05/01 06:00 [pubmed]
PHST- 2021/05/15 06:00 [medline]
PHST- 2021/04/24 00:00 [pmc-release]
AID - ijms22094456 [pii]
AID - ijms-22-04456 [pii]
AID - 10.3390/ijms22094456 [doi]
PST - epublish
SO  - Int J Mol Sci. 2021 Apr 24;22(9):4456. doi: 10.3390/ijms22094456.