PMID- 33923464
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20210518
IS  - 2076-393X (Print)
IS  - 2076-393X (Electronic)
IS  - 2076-393X (Linking)
VI  - 9
IP  - 4
DP  - 2021 Apr 16
TI  - Phenotypic and Genetic Evolutions of a Porcine Reproductive and Respiratory 
      Syndrome Modified Live Vaccine after Limited Passages in Pigs.
LID - 10.3390/vaccines9040392 [doi]
LID - 392
AB  - Modified live vaccines (MLVs) against the porcine reproductive and respiratory 
      syndrome virus (PRRSV) have been regularly associated with safety issues, such as 
      reversion to virulence. In order to characterize the phenotypic and genetic 
      evolution of the PRRSV-1 DV strain from the Porcilis((R)) PRRS MLV after limited 
      passages in pigs, three in vivo experiments were performed. Trial#1 aimed (i) at 
      studying transmission of the vaccine strain from vaccinated to unvaccinated 
      contact pigs. Trial#2 and Trial#3 were designed (ii) to assess the 
      reproducibility of Trial#1, using another vaccine batch, and (iii) to compare the 
      virulence levels of two DV strains isolated from vaccinated (passage one) and 
      diseased contact pigs (passage two) from Trial#1. DV strain isolates from 
      vaccinated and contact pigs from Trial#1 and Trial#2 were submitted to 
      Next-Generation Sequencing (NGS) full-genome sequencing. All contact animals from 
      Trial#1 were infected and showed significantly increased viremia compared to 
      vaccinated pigs, whereas no such change was observed during Trial#2. In Trial#3, 
      viremia and transmission were higher for inoculated pigs with passage two of the 
      DV strain, compared with passage one. In this study, we showed that the 
      re-adaptation of the DV strain to pigs is associated with faster replication and 
      increased transmission of the vaccine strain. Punctually, a decrease of 
      attenuation of the DV vaccine strain associated with clinical signs and increased 
      viremia may occur after limited passages in pigs. Furthermore, we identified 
      three mutations linked to pig re-adaptation and five other mutations as potential 
      virulence determinants.
FAU - Eclercy, Julie
AU  - Eclercy J
AD  - Agence Nationale de Securite Sanitaire de l'Alimentation, de l'Environnement et 
      du Travail (Anses), Laboratoire de Ploufragan-Plouzane-Niort, BP 53, 22440 
      Ploufragan, France.
FAU - Renson, Patricia
AU  - Renson P
AUID- ORCID: 0000-0001-8329-8479
AD  - Agence Nationale de Securite Sanitaire de l'Alimentation, de l'Environnement et 
      du Travail (Anses), Laboratoire de Ploufragan-Plouzane-Niort, BP 53, 22440 
      Ploufragan, France.
FAU - Hirchaud, Edouard
AU  - Hirchaud E
AD  - Agence Nationale de Securite Sanitaire de l'Alimentation, de l'Environnement et 
      du Travail (Anses), Laboratoire de Ploufragan-Plouzane-Niort, BP 53, 22440 
      Ploufragan, France.
FAU - Andraud, Mathieu
AU  - Andraud M
AUID- ORCID: 0000-0003-2891-2901
AD  - Agence Nationale de Securite Sanitaire de l'Alimentation, de l'Environnement et 
      du Travail (Anses), Laboratoire de Ploufragan-Plouzane-Niort, BP 53, 22440 
      Ploufragan, France.
FAU - Beven, Veronique
AU  - Beven V
AD  - Agence Nationale de Securite Sanitaire de l'Alimentation, de l'Environnement et 
      du Travail (Anses), Laboratoire de Ploufragan-Plouzane-Niort, BP 53, 22440 
      Ploufragan, France.
FAU - Paboeuf, Frederic
AU  - Paboeuf F
AD  - Agence Nationale de Securite Sanitaire de l'Alimentation, de l'Environnement et 
      du Travail (Anses), Laboratoire de Ploufragan-Plouzane-Niort, BP 53, 22440 
      Ploufragan, France.
FAU - Rose, Nicolas
AU  - Rose N
AD  - Agence Nationale de Securite Sanitaire de l'Alimentation, de l'Environnement et 
      du Travail (Anses), Laboratoire de Ploufragan-Plouzane-Niort, BP 53, 22440 
      Ploufragan, France.
FAU - Blanchard, Yannick
AU  - Blanchard Y
AD  - Agence Nationale de Securite Sanitaire de l'Alimentation, de l'Environnement et 
      du Travail (Anses), Laboratoire de Ploufragan-Plouzane-Niort, BP 53, 22440 
      Ploufragan, France.
FAU - Bourry, Olivier
AU  - Bourry O
AUID- ORCID: 0000-0003-0220-0922
AD  - Agence Nationale de Securite Sanitaire de l'Alimentation, de l'Environnement et 
      du Travail (Anses), Laboratoire de Ploufragan-Plouzane-Niort, BP 53, 22440 
      Ploufragan, France.
LA  - eng
PT  - Journal Article
DEP - 20210416
PL  - Switzerland
TA  - Vaccines (Basel)
JT  - Vaccines
JID - 101629355
PMC - PMC8073166
OTO - NOTNLM
OT  - PRRS virus
OT  - attenuation
OT  - modified live vaccine
OT  - mutation
OT  - variants
COIS- The authors declare no conflict of interest.
EDAT- 2021/05/01 06:00
MHDA- 2021/05/01 06:01
PMCR- 2021/04/16
CRDT- 2021/04/30 01:25
PHST- 2021/02/26 00:00 [received]
PHST- 2021/03/15 00:00 [revised]
PHST- 2021/04/11 00:00 [accepted]
PHST- 2021/04/30 01:25 [entrez]
PHST- 2021/05/01 06:00 [pubmed]
PHST- 2021/05/01 06:01 [medline]
PHST- 2021/04/16 00:00 [pmc-release]
AID - vaccines9040392 [pii]
AID - vaccines-09-00392 [pii]
AID - 10.3390/vaccines9040392 [doi]
PST - epublish
SO  - Vaccines (Basel). 2021 Apr 16;9(4):392. doi: 10.3390/vaccines9040392.