PMID- 33923636
OWN - NLM
STAT- MEDLINE
DCOM- 20210512
LR  - 20210512
IS  - 1422-0067 (Electronic)
IS  - 1422-0067 (Linking)
VI  - 22
IP  - 8
DP  - 2021 Apr 16
TI  - Chemical, Thermal, Time, and Enzymatic Stability of Silk Materials with Silk I 
      Structure.
LID - 10.3390/ijms22084136 [doi]
LID - 4136
AB  - The crystalline structure of silk fibroin Silk I is generally considered to be a 
      metastable structure; however, there is no definite conclusion under what 
      circumstances this crystalline structure is stable or the crystal form will 
      change. In this study, silk fibroin solution was prepared from B. Mori silkworm 
      cocoons, and a combined method of freeze-crystallization and freeze-drying at 
      different temperatures was used to obtain stable Silk I crystalline material and 
      uncrystallized silk material, respectively. Different concentrations of methanol 
      and ethanol were used to soak the two materials with different time periods to 
      investigate the effect of immersion treatments on the crystalline structure of 
      silk fibroin materials. X-ray diffraction (XRD), Fourier transform infrared 
      spectroscopy (FTIR), Raman scattering spectroscopy (Raman), Scanning electron 
      microscope (SEM), and Thermogravimetric analysis (TGA) were used to characterize 
      the structure of silk fibroin before and after the treatments. The results showed 
      that, after immersion treatments, uncrystallized silk fibroin material with 
      random coil structure was transformed into Silk II crystal structure, while the 
      silk material with dominated Silk I crystal structure showed good long-term 
      stability without obvious transition to Silk II crystal structure. alpha-chymotrypsin 
      biodegradation study showed that the crystalline structure of silk fibroin Silk I 
      materials is enzymatically degradable with a much lower rate compared to 
      uncrystallized silk materials. The crystalline structure of Silk I materials 
      demonstrate a good long-term stability, endurance to alcohol sterilization 
      without structural changes, and can be applied to many emerging fields, such as 
      biomedical materials, sustainable materials, and biosensors.
FAU - Zhao, Meihui
AU  - Zhao M
AD  - National Engineering Laboratory for Modern Silk, College of Textile and Clothing 
      Engineering, Soochow University, Suzhou 215123, China.
FAU - Qi, Zhenzhen
AU  - Qi Z
AD  - National Engineering Laboratory for Modern Silk, College of Textile and Clothing 
      Engineering, Soochow University, Suzhou 215123, China.
FAU - Tao, Xiaosheng
AU  - Tao X
AD  - National Engineering Laboratory for Modern Silk, College of Textile and Clothing 
      Engineering, Soochow University, Suzhou 215123, China.
FAU - Newkirk, Chad
AU  - Newkirk C
AD  - Department of Physics and Astronomy, Rowan University, Glassboro, NJ 08028, USA.
AD  - Department of Molecular and Cellular Biosciences, Rowan University, Glassboro, NJ 
      08028, USA.
FAU - Hu, Xiao
AU  - Hu X
AUID- ORCID: 0000-0002-2579-2820
AD  - Department of Physics and Astronomy, Rowan University, Glassboro, NJ 08028, USA.
AD  - Department of Molecular and Cellular Biosciences, Rowan University, Glassboro, NJ 
      08028, USA.
FAU - Lu, Shenzhou
AU  - Lu S
AUID- ORCID: 0000-0003-4188-2057
AD  - National Engineering Laboratory for Modern Silk, College of Textile and Clothing 
      Engineering, Soochow University, Suzhou 215123, China.
LA  - eng
GR  - 51973144/National Natural Science Foundation of China/
GR  - SWYY-038/Six Talent Peaks Project in Jiangsu Province/
GR  - 20KJA540002/College Nature Science Research Project of Jiangsu Province/
GR  - DMR-1809541/US Natural Science Foundation Biomaterials Program/
PT  - Journal Article
DEP - 20210416
PL  - Switzerland
TA  - Int J Mol Sci
JT  - International journal of molecular sciences
JID - 101092791
RN  - 9007-76-5 (Fibroins)
RN  - EC 3.4.21.1 (Chymotrypsin)
SB  - IM
MH  - Chymotrypsin/metabolism
MH  - Fibroins/*chemistry/standards
MH  - Hot Temperature
MH  - Protein Denaturation
MH  - Protein Stability
MH  - Proteolysis
PMC - PMC8073524
OTO - NOTNLM
OT  - crystalline structure
OT  - porous material
OT  - silk fibroin
OT  - stability
COIS- The authors declare no competing financial interest.
EDAT- 2021/05/01 06:00
MHDA- 2021/05/13 06:00
PMCR- 2021/04/16
CRDT- 2021/04/30 01:25
PHST- 2021/03/09 00:00 [received]
PHST- 2021/04/10 00:00 [revised]
PHST- 2021/04/13 00:00 [accepted]
PHST- 2021/04/30 01:25 [entrez]
PHST- 2021/05/01 06:00 [pubmed]
PHST- 2021/05/13 06:00 [medline]
PHST- 2021/04/16 00:00 [pmc-release]
AID - ijms22084136 [pii]
AID - ijms-22-04136 [pii]
AID - 10.3390/ijms22084136 [doi]
PST - epublish
SO  - Int J Mol Sci. 2021 Apr 16;22(8):4136. doi: 10.3390/ijms22084136.