PMID- 33924516
OWN - NLM
STAT- MEDLINE
DCOM- 20210512
LR  - 20240401
IS  - 1422-0067 (Electronic)
IS  - 1422-0067 (Linking)
VI  - 22
IP  - 8
DP  - 2021 Apr 13
TI  - Microscopic Methods for Identification of Sulfate-Reducing Bacteria from Various 
      Habitats.
LID - 10.3390/ijms22084007 [doi]
LID - 4007
AB  - This paper is devoted to microscopic methods for the identification of 
      sulfate-reducing bacteria (SRB). In this context, it describes various habitats, 
      morphology and techniques used for the detection and identification of this very 
      heterogeneous group of anaerobic microorganisms. SRB are present in almost every 
      habitat on Earth, including freshwater and marine water, soils, sediments or 
      animals. In the oil, water and gas industries, they can cause considerable 
      economic losses due to their hydrogen sulfide production; in periodontal lesions 
      and the colon of humans, they can cause health complications. Although the role 
      of these bacteria in inflammatory bowel diseases is not entirely known yet, their 
      presence is increased in patients and produced hydrogen sulfide has a cytotoxic 
      effect. For these reasons, methods for the detection of these microorganisms were 
      described. Apart from selected molecular techniques, including metagenomics, 
      fluorescence microscopy was one of the applied methods. Especially fluorescence 
      in situ hybridization (FISH) in various modifications was described. This method 
      enables visual identification of SRB, determining their abundance and spatial 
      distribution in environmental biofilms and gut samples.
FAU - Kushkevych, Ivan
AU  - Kushkevych I
AUID- ORCID: 0000-0002-8450-7137
AD  - Department of Experimental Biology, Faculty of Science, Masaryk University, 62500 
      Brno, Czech Republic.
FAU - Hyzova, Blanka
AU  - Hyzova B
AD  - Department of Experimental Biology, Faculty of Science, Masaryk University, 62500 
      Brno, Czech Republic.
FAU - Vitezova, Monika
AU  - Vitezova M
AUID- ORCID: 0000-0002-0999-7957
AD  - Department of Experimental Biology, Faculty of Science, Masaryk University, 62500 
      Brno, Czech Republic.
FAU - Rittmann, Simon K-M R
AU  - Rittmann SKR
AUID- ORCID: 0000-0002-9746-3284
AD  - Archaea Physiology & Biotechnology Group, Department of Functional and 
      Evolutionary Ecology, Universitat Wien, 1090 Wien, Austria.
LA  - eng
GR  - MUNI/A/1425/2020/Masarykova Univerzita/
PT  - Journal Article
PT  - Review
DEP - 20210413
PL  - Switzerland
TA  - Int J Mol Sci
JT  - International journal of molecular sciences
JID - 101092791
RN  - 0 (Sulfates)
SB  - IM
MH  - Animals
MH  - Bacteria/*metabolism
MH  - *Ecosystem
MH  - Humans
MH  - Metagenomics
MH  - Microscopy/*methods
MH  - Oxidation-Reduction
MH  - Sulfates/*metabolism
PMC - PMC8069399
OTO - NOTNLM
OT  - DAPI
OT  - Desulfovibrio
OT  - FISH
OT  - IBD
OT  - SRB
OT  - SRM
OT  - SRP
OT  - anaerobic microorganisms
OT  - fluorescence microscopy
OT  - gut microbiota
OT  - habitats
OT  - identification
OT  - microscopy
OT  - sulfate reduction
COIS- The authors declare no conflict of interest.
EDAT- 2021/05/01 06:00
MHDA- 2021/05/13 06:00
PMCR- 2021/04/13
CRDT- 2021/04/30 01:28
PHST- 2021/02/26 00:00 [received]
PHST- 2021/03/31 00:00 [revised]
PHST- 2021/04/03 00:00 [accepted]
PHST- 2021/04/30 01:28 [entrez]
PHST- 2021/05/01 06:00 [pubmed]
PHST- 2021/05/13 06:00 [medline]
PHST- 2021/04/13 00:00 [pmc-release]
AID - ijms22084007 [pii]
AID - ijms-22-04007 [pii]
AID - 10.3390/ijms22084007 [doi]
PST - epublish
SO  - Int J Mol Sci. 2021 Apr 13;22(8):4007. doi: 10.3390/ijms22084007.