PMID- 33924583
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20210518
IS  - 2227-9059 (Print)
IS  - 2227-9059 (Electronic)
IS  - 2227-9059 (Linking)
VI  - 9
IP  - 4
DP  - 2021 Apr 13
TI  - Phaseolin Attenuates Lipopolysaccharide-Induced Inflammation in RAW 264.7 Cells 
      and Zebrafish.
LID - 10.3390/biomedicines9040420 [doi]
LID - 420
AB  - Kushen (Radix Sophorae flavescentis) is used to treat ulcerative colitis, tumors, 
      and pruritus. Recently, phaseolin, formononetin, matrine, luteolin, and 
      quercetin, through a network pharmacology approach, were tentatively identified 
      as five bioactive constituents responsible for the anti-inflammatory effects of 
      S. flavescentis. However, the role of phaseolin (one of the primary components of 
      S. flavescentis) in the direct regulation of inflammation and inflammatory 
      processes is not well known. In this study, the beneficial role of phaseolin 
      against inflammation was explored in lipopolysaccharide (LPS)-induced 
      inflammation models of RAW 264.7 macrophages and zebrafish larvae. Phaseolin 
      inhibited LPS-mediated production of nitric oxide (NO) and the expression of 
      inducible nitric oxide synthase (iNOS), without affecting cell viability. In 
      addition, phaseolin suppressed pro-inflammatory mediators such as cyclooxygenase 
      2 (COX-2), interleukin-1beta (IL-1beta), tumor necrosis factor alpha (TNF-alpha), monocyte 
      chemoattractant protein-1 (MCP-1), and interleukin-6 (IL-6) in a dose-dependent 
      manner. Furthermore, phaseolin reduced matrix metalloproteinase (MMP) activity as 
      well as macrophage adhesion in vitro and the recruitment of leukocytes in vivo by 
      downregulating Ninjurin 1 (Ninj1), an adhesion molecule. Finally, phaseolin 
      inhibited the nuclear translocation of nuclear factor-kappa B (NF-kappaB). In view of 
      the above, our results suggest that phaseolin could be a potential therapeutic 
      candidate for the management of inflammation.
FAU - Hwang, Su-Jung
AU  - Hwang SJ
AD  - School of Pharmacy, Sungkyunkwan University, 2066 Seobu-ro, Jangan-gu, Suwon 
      16419, Gyeonggi-do, Korea.
FAU - Song, Ye-Seul
AU  - Song YS
AD  - School of Pharmacy, Sungkyunkwan University, 2066 Seobu-ro, Jangan-gu, Suwon 
      16419, Gyeonggi-do, Korea.
FAU - Lee, Hyo-Jong
AU  - Lee HJ
AUID- ORCID: 0000-0003-4826-7954
AD  - School of Pharmacy, Sungkyunkwan University, 2066 Seobu-ro, Jangan-gu, Suwon 
      16419, Gyeonggi-do, Korea.
LA  - eng
GR  - 2019R1C1C1003558/National Research Foundation of Korea/
GR  - 2019R1A6A3A01091754/National Research Foundation of Korea/
GR  - 2021M3A9C4000960/National Research Foundation of Korea/
PT  - Journal Article
DEP - 20210413
PL  - Switzerland
TA  - Biomedicines
JT  - Biomedicines
JID - 101691304
PMC - PMC8069760
OTO - NOTNLM
OT  - Kushen
OT  - Radix Sophorae flavescentis
OT  - inflammation
OT  - phaseolin
OT  - zebrafish
COIS- The authors declare no conflict of interest.
EDAT- 2021/05/01 06:00
MHDA- 2021/05/01 06:01
PMCR- 2021/04/13
CRDT- 2021/04/30 01:29
PHST- 2021/02/23 00:00 [received]
PHST- 2021/04/07 00:00 [revised]
PHST- 2021/04/09 00:00 [accepted]
PHST- 2021/04/30 01:29 [entrez]
PHST- 2021/05/01 06:00 [pubmed]
PHST- 2021/05/01 06:01 [medline]
PHST- 2021/04/13 00:00 [pmc-release]
AID - biomedicines9040420 [pii]
AID - biomedicines-09-00420 [pii]
AID - 10.3390/biomedicines9040420 [doi]
PST - epublish
SO  - Biomedicines. 2021 Apr 13;9(4):420. doi: 10.3390/biomedicines9040420.