PMID- 33925352
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20210527
IS  - 2079-6382 (Print)
IS  - 2079-6382 (Electronic)
IS  - 2079-6382 (Linking)
VI  - 10
IP  - 5
DP  - 2021 Apr 27
TI  - Distribution of beta-Lactamase Genes in Clinical Isolates from California Central 
      Valley Hospital Deviates from the United States Nationwide Trends.
LID - 10.3390/antibiotics10050498 [doi]
LID - 498
AB  - The evolution and dissemination of antibiotic resistance genes throughout the 
      world are clearly affected by the selection and migration of resistant bacteria. 
      However, the relative contributions of selection and migration at a local scale 
      have not been fully explored. We sought to identify which of these factors has 
      the strongest effect through comparisons of antibiotic resistance gene abundance 
      between a distinct location and its surroundings over an extended period of six 
      years. In this work, we used two repositories of extended spectrum beta-lactamase 
      (ESBL)-producing isolates collected since 2013 from patients at Dignity Health 
      Mercy Medical Center (DHMMC) in Merced, California, USA, and a nationwide 
      database compiled from clinical isolate genomes reported by the National Center 
      for Biotechnology Information (NCBI) since 2013. We analyzed the stability of 
      average resistance gene frequencies over the years since collection of these 
      clinical isolates began for each repository. We then compared the frequencies of 
      resistance genes in the DHMMC collection with the averages of the nationwide 
      frequencies. We found DHMMC gene frequencies are stable over time and differ 
      significantly from nationwide frequencies throughout the period of time we 
      examined. Our results suggest that local selective pressures are a more important 
      influence on the population structure of resistance genes in bacterial 
      populations than migration. This, in turn, indicates the potential for antibiotic 
      resistance to be controlled at a regional level, making it easier to limit the 
      spread through local stewardship.
FAU - Guzman-Cole, Candace
AU  - Guzman-Cole C
AD  - Department of Molecular and Cell Biology, School of Natural Sciences, University 
      of California Merced, Merced, CA 95343, USA.
FAU - Santiago, Fabian
AU  - Santiago F
AD  - Department of Applied Mathematics, School of Natural Sciences, University of 
      California, Merced, CA 95343, USA.
FAU - Garsevanyan, Sona
AU  - Garsevanyan S
AD  - Department of Molecular and Cell Biology, School of Natural Sciences, University 
      of California Merced, Merced, CA 95343, USA.
FAU - Sindi, Suzanne
AU  - Sindi S
AD  - Department of Applied Mathematics, School of Natural Sciences, University of 
      California, Merced, CA 95343, USA.
FAU - Barlow, Miriam
AU  - Barlow M
AD  - Department of Molecular and Cell Biology, School of Natural Sciences, University 
      of California Merced, Merced, CA 95343, USA.
LA  - eng
PT  - Journal Article
DEP - 20210427
PL  - Switzerland
TA  - Antibiotics (Basel)
JT  - Antibiotics (Basel, Switzerland)
JID - 101637404
PMC - PMC8146836
OTO - NOTNLM
OT  - ESBL
OT  - Enterobacteriaceae
OT  - antibiotic resistance
OT  - selection
OT  - stewardship
COIS- The authors declare no conflict of interest.
EDAT- 2021/05/01 06:00
MHDA- 2021/05/01 06:01
PMCR- 2021/04/27
CRDT- 2021/04/30 01:31
PHST- 2021/03/16 00:00 [received]
PHST- 2021/04/17 00:00 [revised]
PHST- 2021/04/22 00:00 [accepted]
PHST- 2021/04/30 01:31 [entrez]
PHST- 2021/05/01 06:00 [pubmed]
PHST- 2021/05/01 06:01 [medline]
PHST- 2021/04/27 00:00 [pmc-release]
AID - antibiotics10050498 [pii]
AID - antibiotics-10-00498 [pii]
AID - 10.3390/antibiotics10050498 [doi]
PST - epublish
SO  - Antibiotics (Basel). 2021 Apr 27;10(5):498. doi: 10.3390/antibiotics10050498.