PMID- 33928643
OWN - NLM
STAT- MEDLINE
DCOM- 20211213
LR  - 20240226
IS  - 1097-4652 (Electronic)
IS  - 0021-9541 (Linking)
VI  - 236
IP  - 11
DP  - 2021 Nov
TI  - 2,4-DCP compromises the fertilization capacity of mouse oocytes.
PG  - 7605-7611
LID - 10.1002/jcp.30403 [doi]
AB  - 2,4-DCP (2,4-dichlorophenol) is an environmental estrogen that is ubiquitously 
      distributed in the environment and widely used to produce herbicides and 
      pharmaceutical intermediates. Although 2,4-DCP is suspected to have endocrine 
      disruption, the reproductive toxicity of 2,4-DCP in mammals has not been 
      adequately assessed. In the present study, we examined the effect of 2,4-DCP on 
      the fertility of mouse eggs. The data showed that oral administration of 2,4-DCP 
      (180 mg/kg/day for 7 days) compromises the fertilization rate of mouse oocytes. 
      To further analyze the mechanism by which 2,4-DCP decreases fertilization, the 
      key regulators and events during fertilization of mouse eggs were investigated. 
      We found that the dynamics of cortical granules (CGs) were disrupted by showing 
      the redistribution of CG free domain in 2,4-DCP-administered oocytes. This 
      abnormality perturbed the sperm binding site in the zona pellucida (ZP) and 
      dramatically reduced the number of sperm binding to the ZP of 
      2,4-DCP-administered oocytes. In addition, the abundance of Juno, a sperm 
      receptor on the egg membrane, was also decreased and its distribution was 
      mislocated in 2,4-DCP-administered oocytes. Finally, we validated that the 
      defects of fertilization participants and events caused by 2,4-DCP might be 
      mediated by the increased level of reactive oxygen species-induced apoptosis of 
      oocytes. Therefore, we demonstrate that 2,4-DCP compromises the fertilization 
      ability of mouse oocytes via inducing oxidative stress.
CI  - (c) 2021 Wiley Periodicals LLC.
FAU - Dai, Xiaoxin
AU  - Dai X
AD  - Department of Aquaculture, School of Fisheries, Zhejiang Ocean University, 
      Zhoushan, Zhejiang, China.
FAU - Qiu, Lijun
AU  - Qiu L
AD  - Department of Aquaculture, School of Fisheries, Zhejiang Ocean University, 
      Zhoushan, Zhejiang, China.
FAU - Rashida, Churi
AU  - Rashida C
AD  - Department of Aquaculture, School of Fisheries, Zhejiang Ocean University, 
      Zhoushan, Zhejiang, China.
FAU - Xu, Chunying
AU  - Xu C
AD  - Animal Husbandry and Veterinary Development Center, Zhoushan Municipal Bureau of 
      Agricultural and Rural Affairs, Zhoushan People's Government, Zhoushan, Zhejiang, 
      China.
FAU - Mu, Yi
AU  - Mu Y
AD  - Department of Aquaculture, School of Fisheries, Zhejiang Ocean University, 
      Zhoushan, Zhejiang, China.
FAU - Gao, Yang
AU  - Gao Y
AD  - Department of Aquaculture, School of Fisheries, Zhejiang Ocean University, 
      Zhoushan, Zhejiang, China.
FAU - Chu, Zhangjie
AU  - Chu Z
AD  - Department of Aquaculture, School of Fisheries, Zhejiang Ocean University, 
      Zhoushan, Zhejiang, China.
FAU - Zhao, Bo
AU  - Zhao B
AUID- ORCID: 0000-0003-3072-0757
AD  - Department of Aquaculture, School of Fisheries, Zhejiang Ocean University, 
      Zhoushan, Zhejiang, China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20210430
PL  - United States
TA  - J Cell Physiol
JT  - Journal of cellular physiology
JID - 0050222
RN  - 0 (Chlorophenols)
RN  - 0 (Endocrine Disruptors)
RN  - 0 (Reactive Oxygen Species)
RN  - 0 (Receptors, Cell Surface)
RN  - 0 (folate receptor 4, mouse)
RN  - R669TG1950 (2,4-dichlorophenol)
SB  - IM
MH  - Animals
MH  - Apoptosis/drug effects
MH  - Chlorophenols/*toxicity
MH  - Cytoplasmic Granules/*drug effects/metabolism
MH  - Endocrine Disruptors/*toxicity
MH  - Female
MH  - Fertilization in Vitro
MH  - Mice, Inbred ICR
MH  - Oocytes/*drug effects/metabolism
MH  - Oxidative Stress/drug effects
MH  - Protein Transport
MH  - Reactive Oxygen Species/metabolism
MH  - Receptors, Cell Surface/metabolism
MH  - Sperm-Ovum Interactions/*drug effects
MH  - Mice
OTO - NOTNLM
OT  - 2, 4-DCP
OT  - cortical granule
OT  - fertilization
OT  - juno
OT  - mouse eggs
EDAT- 2021/05/01 06:00
MHDA- 2021/12/15 06:00
CRDT- 2021/04/30 07:10
PHST- 2021/04/02 00:00 [revised]
PHST- 2021/01/05 00:00 [received]
PHST- 2021/04/07 00:00 [accepted]
PHST- 2021/05/01 06:00 [pubmed]
PHST- 2021/12/15 06:00 [medline]
PHST- 2021/04/30 07:10 [entrez]
AID - 10.1002/jcp.30403 [doi]
PST - ppublish
SO  - J Cell Physiol. 2021 Nov;236(11):7605-7611. doi: 10.1002/jcp.30403. Epub 2021 Apr 
      30.