PMID- 33928963
OWN - NLM
STAT- MEDLINE
DCOM- 20210514
LR  - 20230920
IS  - 2040-3372 (Electronic)
IS  - 2040-3364 (Print)
IS  - 2040-3364 (Linking)
VI  - 13
IP  - 16
DP  - 2021 Apr 30
TI  - Gold nanoparticles (AuNPs) impair LPS-driven immune responses by promoting a 
      tolerogenic-like dendritic cell phenotype with altered endosomal structures.
PG  - 7648-7666
LID - 10.1039/d0nr09153g [doi]
AB  - Dendritic cells (DCs) shape immune responses by influencing T-cell activation. 
      Thus, they are considered both an interesting model for studying nano-immune 
      interactions and a promising target for nano-based biomedical applications. 
      However, the accentuated ability of nanoparticles (NPs) to interact with 
      biomolecules may have an impact on DC function that poses an unexpected risk of 
      unbalanced immune reactions. Here, we investigated the potential effects of gold 
      nanoparticles (AuNPs) on DC function and the consequences for effector and memory 
      T-cell responses in the presence of the microbial inflammatory stimulus 
      lipopolysaccharide (LPS). Overall, we found that, in the absence of LPS, none of 
      the tested NPs induced a DC response. However, whereas 4-, 8-, and 11 nm AuNPs 
      did not modulate LPS-dependent immune responses, 26 nm AuNPs shifted the 
      phenotype of LPS-activated DCs toward a tolerogenic state, characterized by 
      downregulation of CD86, IL-12 and IL-27, upregulation of ILT3, and induction of 
      class E compartments. Moreover, this DC phenotype was less proficient in 
      promoting Th1 activation and central memory T-cell proliferation. Taken together, 
      these findings support the perception that AuNPs are safe under homeostatic 
      conditions; however, particular care should be taken in patients experiencing a 
      current infection or disorders of the immune system.
FAU - Michelini, Sara
AU  - Michelini S
AUID- ORCID: 0000-0001-9470-3337
AD  - Department of Biosciences, Paris-Lodron University Salzburg, Hellbrunner Str. 34, 
      5020 Salzburg, Austria. jutta.horejs_hoeck@sbg.ac.at.
FAU - Barbero, Francesco
AU  - Barbero F
AUID- ORCID: 0000-0001-8704-0651
AD  - Insitut Catala de Nanosciencia i Nanotecnologia (ICN2), UAB Campus, Bellaterra, 
      Barcelona 08193, Spain.
FAU - Prinelli, Alessandra
AU  - Prinelli A
AD  - AvantiCell Science Ltd, Gibbsyard Building, Auchincruive, Ayr, KA6 5HW, UK.
FAU - Steiner, Philip
AU  - Steiner P
AD  - Department of Biosciences, Paris-Lodron University Salzburg, Hellbrunner Str. 34, 
      5020 Salzburg, Austria. jutta.horejs_hoeck@sbg.ac.at.
FAU - Weiss, Richard
AU  - Weiss R
AUID- ORCID: 0000-0003-3185-7253
AD  - Department of Biosciences, Paris-Lodron University Salzburg, Hellbrunner Str. 34, 
      5020 Salzburg, Austria. jutta.horejs_hoeck@sbg.ac.at.
FAU - Verwanger, Thomas
AU  - Verwanger T
AD  - Department of Biosciences, Paris-Lodron University Salzburg, Hellbrunner Str. 34, 
      5020 Salzburg, Austria. jutta.horejs_hoeck@sbg.ac.at.
FAU - Andosch, Ancuela
AU  - Andosch A
AD  - Department of Biosciences, Paris-Lodron University Salzburg, Hellbrunner Str. 34, 
      5020 Salzburg, Austria. jutta.horejs_hoeck@sbg.ac.at.
FAU - Lutz-Meindl, Ursula
AU  - Lutz-Meindl U
AD  - Department of Biosciences, Paris-Lodron University Salzburg, Hellbrunner Str. 34, 
      5020 Salzburg, Austria. jutta.horejs_hoeck@sbg.ac.at.
FAU - Puntes, Victor F
AU  - Puntes VF
AUID- ORCID: 0000-0001-8996-9499
AD  - Insitut Catala de Nanosciencia i Nanotecnologia (ICN2), UAB Campus, Bellaterra, 
      Barcelona 08193, Spain.
FAU - Drobne, Damjana
AU  - Drobne D
AUID- ORCID: 0000-0002-5970-7460
AD  - Biotechnical Faculty, University of Ljubljana, Vecna pot 111, 1000 Ljubljana, 
      Slovenia.
FAU - Duschl, Albert
AU  - Duschl A
AUID- ORCID: 0000-0002-7034-9860
AD  - Department of Biosciences, Paris-Lodron University Salzburg, Hellbrunner Str. 34, 
      5020 Salzburg, Austria. jutta.horejs_hoeck@sbg.ac.at.
FAU - Horejs-Hoeck, Jutta
AU  - Horejs-Hoeck J
AUID- ORCID: 0000-0002-0984-204X
AD  - Department of Biosciences, Paris-Lodron University Salzburg, Hellbrunner Str. 34, 
      5020 Salzburg, Austria. jutta.horejs_hoeck@sbg.ac.at.
LA  - eng
PT  - Journal Article
PL  - England
TA  - Nanoscale
JT  - Nanoscale
JID - 101525249
RN  - 0 (Lipopolysaccharides)
RN  - 7440-57-5 (Gold)
SB  - IM
MH  - Dendritic Cells
MH  - *Gold
MH  - Humans
MH  - Lipopolysaccharides
MH  - *Metal Nanoparticles/toxicity
MH  - Phenotype
PMC - PMC8087175
COIS- There are no conflicts to declare.
EDAT- 2021/05/01 06:00
MHDA- 2021/05/15 06:00
PMCR- 2021/04/15
CRDT- 2021/04/30 08:48
PHST- 2021/04/30 08:48 [entrez]
PHST- 2021/05/01 06:00 [pubmed]
PHST- 2021/05/15 06:00 [medline]
PHST- 2021/04/15 00:00 [pmc-release]
AID - d0nr09153g [pii]
AID - 10.1039/d0nr09153g [doi]
PST - ppublish
SO  - Nanoscale. 2021 Apr 30;13(16):7648-7666. doi: 10.1039/d0nr09153g.