PMID- 33931990
OWN - NLM
STAT- MEDLINE
DCOM- 20210504
LR  - 20210504
IS  - 1000-0607 (Print)
IS  - 1000-0607 (Linking)
VI  - 46
IP  - 4
DP  - 2021 Apr 25
TI  - [Intervention mechanism of moxa-cone moxibustion at Shu- and Mu-acupoints on the 
      expression of RORgammat/Foxp3 in asthma mice: an analysis based on PI3K signaling 
      pathway].
PG  - 272-7
LID - 10.13702/j.1000-0607.200491 [doi]
AB  - OBJECTIVE: To explore the effect of moxa-cone moxibustion at "Feishu" (BL13) and 
      "Zhongfu" (LU1) on the contents of related inflammatory factors in serum and 
      bronchoalveolar lavage fluid (BALF), and the expression of 
      phosphatidy-linositol-3-kinase (PI3K), retinoic acid related orphan receptor gammat 
      (RORgammat) and fork/wing helix transcription factor 3 (Foxp3) in lung tissue in 
      asthma mice. METHODS: Sixty male Balb/c mice were divided into normal, model, 
      LY294002 (an inhibitor of PI3K, LY), electroacupuncture (EA) and moxa-cone 
      moxibustion (moxibustion) groups (n=12 in each group). The asthma model was 
      established by intraperitoneal injection of ovalbumin sensitization. The mice in 
      the LY group were injected with LY 294002 (7.5 mg/kg) via the tail vein. EA or 
      moxa-cone moxibustion was applied at BL13 and LU1 in the EA or moxibustion group 
      once daily for 2 weeks. The levels of immunoglobulin E (IgE), interleukin-4 
      (IL-4) and interferon-gamma (IFN-gamma) in serum and BALF were detected by enzyme-linked 
      immunosorbent assay. The expressions of PI3K, RORgammat and Foxp3 in lung tissue were 
      detected by real-time fluorescent quantitative polymerase chain reaction (QRT 
      PCR) and immunohistochemistry. RESULTS: Compared with the normal group, the 
      levels of IgE and IL-4 in serum and BALF, the expressions of PI3K and RORgammat mRNA 
      and protein in lung tissue were significantly increased (P<0.01), while the 
      levels of IFN-gamma, and the expressions of Foxp3 mRNA and protein were significantly 
      reduced (P<0.01) in the model group. Compared with the model group, the levels of 
      IgE and IL-4 in serum and BALF, the expressions of PI3K and RORgammat mRNA and 
      protein in lung tissue were significantly decreased (P<0.01, P<0.05), and the 
      levels of IFN-gamma in serum and BALF, the expressions of Foxp3 mRNA and protein in 
      lung tissue were significantly increased (P<0.01) in the LY, EA and moxibustion 
      groups. CONCLUSION: Moxa cone moxibustion at Shu- and Mu-acupoints of the lung 
      meridian can reduce airway inflammatory reaction and control asthma attacks in 
      asthma mice, which is closely related to its effects in regulating the 
      expressions of RORgammat and Foxp3 through PI3K signaling pathway.
FAU - Qiao, Yun
AU  - Qiao Y
AD  - College of Acupuncture and Massage, Guangxi University of Chinese Medicine, 
      Nanning 530200, China.
FAU - Lei, Hui-Ting
AU  - Lei HT
AD  - College of Acupuncture and Massage, Guangxi University of Chinese Medicine, 
      Nanning 530200, China.
FAU - Yi, Wei
AU  - Yi W
AD  - Graduate School, Guangxi University of Chinese Medicine, Nanning 530200, China.
FAU - Zhao, Cai-Jiao
AU  - Zhao CJ
AD  - College of Acupuncture and Massage, Guangxi University of Chinese Medicine, 
      Nanning 530200, China.
FAU - Xia, Xing
AU  - Xia X
AD  - College of Pharmacy, Guangxi University of Chinese Medicine, Nanning 530200, 
      China.
FAU - Zhou, Bei
AU  - Zhou B
AD  - College of Pharmacy, Guangxi University of Chinese Medicine, Nanning 530200, 
      China.
FAU - Rui, Jing-Lin
AU  - Rui JL
AD  - College of Acupuncture and Massage, Guangxi University of Chinese Medicine, 
      Nanning 530200, China.
FAU - Wang, Zeng-Duo
AU  - Wang ZD
AD  - College of Pharmacy, Guangxi University of Chinese Medicine, Nanning 530200, 
      China.
LA  - chi
PT  - Journal Article
PL  - China
TA  - Zhen Ci Yan Jiu
JT  - Zhen ci yan jiu = Acupuncture research
JID - 8507710
RN  - 0 (Forkhead Transcription Factors)
RN  - 0 (Foxp3 protein, mouse)
RN  - 0 (Nuclear Receptor Subfamily 1, Group F, Member 3)
SB  - IM
MH  - Acupuncture Points
MH  - Animals
MH  - *Asthma/genetics/therapy
MH  - Forkhead Transcription Factors/genetics
MH  - Male
MH  - Mice
MH  - *Moxibustion
MH  - Nuclear Receptor Subfamily 1, Group F, Member 3/genetics
MH  - Phosphatidylinositol 3-Kinases/genetics
MH  - Signal Transduction
OTO - NOTNLM
OT  - Asthma
OT  - Moxa-cone moxibustion
OT  - PI3K signaling pathway
OT  - Shu- and Mu-acupoints
EDAT- 2021/05/02 06:00
MHDA- 2021/05/05 06:00
CRDT- 2021/05/01 06:32
PHST- 2021/05/01 06:32 [entrez]
PHST- 2021/05/02 06:00 [pubmed]
PHST- 2021/05/05 06:00 [medline]
AID - 10.13702/j.1000-0607.200491 [doi]
PST - ppublish
SO  - Zhen Ci Yan Jiu. 2021 Apr 25;46(4):272-7. doi: 10.13702/j.1000-0607.200491.