PMID- 33932363
OWN - NLM
STAT- MEDLINE
DCOM- 20210629
LR  - 20240226
IS  - 1873-6254 (Electronic)
IS  - 0001-706X (Linking)
VI  - 220
DP  - 2021 Aug
TI  - Impact of Toxoplasma gondii infection on TM3 Leydig cells: Alterations in 
      testosterone and cytokines levels.
PG  - 105938
LID - S0001-706X(21)00117-0 [pii]
LID - 10.1016/j.actatropica.2021.105938 [doi]
AB  - Leydig cells play pivotal roles in eliciting male characteristics by producing 
      testosterone and any damage to these cells can compromise male fertility 
      Toxoplasma gondii (T. gondii) is an intracellular parasite capable to invade any 
      nucleated cell, including cells from male reproductive system. Herein, we 
      evaluated the capacity of RH strain of T. gondii to infect TM3 Leydig cells and 
      the impact of this infection on testosterone and inflammatory mediators 
      production. We first, by performing adherence, infection, and intracellular 
      proliferation assays, we found a significant increase in the number of infected 
      Leydig cells, peaking 48 h after the infection with T. gondii. Supernatants of 
      TM3 infected cells exhibited, in a time-dependent manner, increased levels of 
      testosterone as well as monocyte chemoattractant protein-1 (MCP-1) and 
      interferon-gamma (IFN-gamma), which is correlated with the robust T. gondii infection. In 
      conclusion, our study provides new insights regarding the harmful effects of T. 
      gondii infection on male reproductive system.
CI  - Copyright (c) 2021 Elsevier B.V. All rights reserved.
FAU - Staurengo-Ferrari, Larissa
AU  - Staurengo-Ferrari L
AD  - Department of Pathological Sciences, State University of Londrina, Rodovia Celso 
      Garcia Cid Pr 445 Km 380, Londrina 86057-970, Brazil.
FAU - Sanfelice, Raquel Arruda da Silva
AU  - Sanfelice RADS
AD  - Laboratory of Immunoparasitology of Neglected Diseases and Cancer-LIDNC, 
      Department, Department of Pathological Sciences, Center of Biological Sciences, 
      State University of Londrina, Londrina, Parana, Brazil.
FAU - de Souza, Julia Bagatim
AU  - de Souza JB
AD  - Department of General Biology, State University of Londrina, Rodovia Celso Garcia 
      Cid Pr 445 Km 380, Londrina 86057-970, Brazil.
FAU - Assolini, Joao Paulo
AU  - Assolini JP
AD  - Laboratory of Immunoparasitology of Neglected Diseases and Cancer-LIDNC, 
      Department, Department of Pathological Sciences, Center of Biological Sciences, 
      State University of Londrina, Londrina, Parana, Brazil.
FAU - Dos Santos, Dayane Priscila
AU  - Dos Santos DP
AD  - Department of Pathological Sciences, State University of Londrina, Rodovia Celso 
      Garcia Cid Pr 445 Km 380, Londrina 86057-970, Brazil; Department of General 
      Biology, State University of Londrina, Rodovia Celso Garcia Cid Pr 445 Km 380, 
      Londrina 86057-970, Brazil.
FAU - Cataneo, Allan Henrique Depieri
AU  - Cataneo AHD
AD  - Laboratory of Molecular Virology, Instituto Carlos Chagas/Fiocruz-PR, Curitiba, 
      Parana Brazil.
FAU - Bordignon, Juliano
AU  - Bordignon J
AD  - Laboratory of Molecular Virology, Instituto Carlos Chagas/Fiocruz-PR, Curitiba, 
      Parana Brazil.
FAU - Conchon-Costa, Ivete
AU  - Conchon-Costa I
AD  - Laboratory of Immunoparasitology of Neglected Diseases and Cancer-LIDNC, 
      Department, Department of Pathological Sciences, Center of Biological Sciences, 
      State University of Londrina, Londrina, Parana, Brazil.
FAU - da Costa, Idessania Nazareth
AU  - da Costa IN
AD  - Laboratory of Immunoparasitology of Neglected Diseases and Cancer-LIDNC, 
      Department, Department of Pathological Sciences, Center of Biological Sciences, 
      State University of Londrina, Londrina, Parana, Brazil.
FAU - Fernandes, Glaura Scantamburlo Alves
AU  - Fernandes GSA
AD  - Department of General Biology, State University of Londrina, Rodovia Celso Garcia 
      Cid Pr 445 Km 380, Londrina 86057-970, Brazil. Electronic address: glaura@uel.br.
LA  - eng
PT  - Journal Article
DEP - 20210428
PL  - Netherlands
TA  - Acta Trop
JT  - Acta tropica
JID - 0370374
RN  - 0 (Chemokine CCL2)
RN  - 3XMK78S47O (Testosterone)
RN  - 82115-62-6 (Interferon-gamma)
SB  - IM
MH  - Animals
MH  - Chemokine CCL2/biosynthesis
MH  - Interferon-gamma/biosynthesis
MH  - Leydig Cells/*parasitology
MH  - Male
MH  - Mice, Inbred BALB C
MH  - Testosterone/*biosynthesis
MH  - Time Factors
MH  - Toxoplasma
MH  - Toxoplasmosis/*metabolism
MH  - Mice
OTO - NOTNLM
OT  - Leydig cells
OT  - Male infertility
OT  - TM3
OT  - Testosterone
OT  - Toxoplasma gondii
EDAT- 2021/05/02 06:00
MHDA- 2021/06/30 06:00
CRDT- 2021/05/01 20:07
PHST- 2021/01/22 00:00 [received]
PHST- 2021/04/19 00:00 [revised]
PHST- 2021/04/21 00:00 [accepted]
PHST- 2021/05/02 06:00 [pubmed]
PHST- 2021/06/30 06:00 [medline]
PHST- 2021/05/01 20:07 [entrez]
AID - S0001-706X(21)00117-0 [pii]
AID - 10.1016/j.actatropica.2021.105938 [doi]
PST - ppublish
SO  - Acta Trop. 2021 Aug;220:105938. doi: 10.1016/j.actatropica.2021.105938. Epub 2021 
      Apr 28.