PMID- 33933681
OWN - NLM
STAT- MEDLINE
DCOM- 20220321
LR  - 20220321
IS  - 1535-9484 (Electronic)
IS  - 1535-9476 (Print)
IS  - 1535-9476 (Linking)
VI  - 20
DP  - 2021
TI  - HLA Class II Presentation Is Specifically Altered at Elevated Temperatures in the 
      B-Lymphoblastic Cell Line JY.
PG  - 100089
LID - S1535-9476(21)00062-1 [pii]
LID - 10.1016/j.mcpro.2021.100089 [doi]
LID - 100089
AB  - Human leukocyte antigen (HLA) molecules play critical roles in our adaptive 
      immune system by signaling a cell's health status to the immune system, through 
      presentation of small peptides. Understanding HLA biology is important because of 
      its prominent role in autoimmune diseases and cancer immunotherapy. Although both 
      the HLA class I and class II antigen processing and presentation pathways have 
      been studied extensively, the fundamental rules in HLA class II antigen 
      presentation still remain less understood. To clarify the mechanistic and 
      adaptive differences between the HLA systems, we challenged a B lymphoblastic 
      cell line (JY), widely used as model system in studying antigen presentation, 
      with a high temperature treatment to mimic a "fever-like state", representing one 
      of the most common physiological responses to infection. In the absence of real 
      invading pathogenic peptides to present, we could focus on delineating the 
      intrinsic HLA pathway adaptations in response to high temperature in this 
      particular cell line. Following a three-pronged approach, we performed 
      quantitative analyses of the proteome, the HLA class I ligandome, as well as the 
      HLA class II ligandome. The data reveals that elevated temperature may already 
      prepare these cells for an immune-like response through increased HLA class II 
      presentation capacity and specific release of, from the invariant chain 
      originating, CLIP peptides. Interestingly, at high temperature, prominent changes 
      in the composition of the CLIP repertoire were observed, with enrichment of 
      peptides containing C-terminal extensions beyond the CLIP-core region. 
      Collectively, these illustrate intriguing temperature sensitive adaptations in 
      this B cell line.
CI  - Copyright (c) 2021 The Authors. Published by Elsevier Inc. All rights reserved.
FAU - Demmers, Laura C
AU  - Demmers LC
AD  - Biomolecular Mass Spectrometry and Proteomics, Bijvoet Center for Biomolecular 
      Research and Utrecht Institute for Pharmaceutical Sciences, Utrecht University, 
      Utrecht, Netherlands; Netherlands Proteomics Centre, Utrecht, Netherlands.
FAU - Wu, Wei
AU  - Wu W
AD  - Biomolecular Mass Spectrometry and Proteomics, Bijvoet Center for Biomolecular 
      Research and Utrecht Institute for Pharmaceutical Sciences, Utrecht University, 
      Utrecht, Netherlands; Netherlands Proteomics Centre, Utrecht, Netherlands.
FAU - Heck, Albert J R
AU  - Heck AJR
AD  - Biomolecular Mass Spectrometry and Proteomics, Bijvoet Center for Biomolecular 
      Research and Utrecht Institute for Pharmaceutical Sciences, Utrecht University, 
      Utrecht, Netherlands; Netherlands Proteomics Centre, Utrecht, Netherlands. 
      Electronic address: a.j.r.heck@uu.nl.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20210429
PL  - United States
TA  - Mol Cell Proteomics
JT  - Molecular & cellular proteomics : MCP
JID - 101125647
RN  - 0 (Histocompatibility Antigens Class I)
RN  - 0 (Histocompatibility Antigens Class II)
RN  - 0 (Ligands)
RN  - 0 (Proteome)
SB  - IM
MH  - Antigen Presentation
MH  - B-Lymphocytes/*immunology
MH  - Cell Line
MH  - Fever/*immunology
MH  - Histocompatibility Antigens Class I/immunology
MH  - Histocompatibility Antigens Class II/*immunology
MH  - Humans
MH  - Ligands
MH  - Proteome
MH  - Temperature
PMC - PMC8724904
OTO - NOTNLM
OT  - CLIP peptide cluster
OT  - HLA class I peptide presentation
OT  - HLA class II peptide presentation
OT  - HLA peptide processing
OT  - immunopeptidomics
OT  - immunoproteomics
OT  - invariant chain
OT  - temperature-induced adaptations
COIS- Conflict of interest The authors declare no competing interests.
EDAT- 2021/05/03 06:00
MHDA- 2022/03/22 06:00
PMCR- 2021/04/29
CRDT- 2021/05/02 20:46
PHST- 2020/12/19 00:00 [received]
PHST- 2021/03/25 00:00 [revised]
PHST- 2021/04/13 00:00 [accepted]
PHST- 2021/05/03 06:00 [pubmed]
PHST- 2022/03/22 06:00 [medline]
PHST- 2021/05/02 20:46 [entrez]
PHST- 2021/04/29 00:00 [pmc-release]
AID - S1535-9476(21)00062-1 [pii]
AID - 100089 [pii]
AID - 10.1016/j.mcpro.2021.100089 [doi]
PST - ppublish
SO  - Mol Cell Proteomics. 2021;20:100089. doi: 10.1016/j.mcpro.2021.100089. Epub 2021 
      Apr 29.