PMID- 33935993
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20240401
IS  - 1664-302X (Print)
IS  - 1664-302X (Electronic)
IS  - 1664-302X (Linking)
VI  - 12
DP  - 2021
TI  - Drought Exposed Burkholderia seminalis JRBHU6 Exhibits Antimicrobial Potential 
      Through Pyrazine-1,4-Dione Derivatives Targeting Multiple Bacterial and Fungal 
      Proteins.
PG  - 633036
LID - 10.3389/fmicb.2021.633036 [doi]
LID - 633036
AB  - The present study aimed to explore the antimicrobial potentials of soil bacteria 
      and identify the bioactive compounds and their likely targets through in silico 
      studies. A total 53 bacterial isolates were screened for their antimicrobial 
      potential of which the strain JRBHU6 showing highest antimicrobial activity was 
      identified as Burkholderia seminalis (GenBank accession no. MK500868) based on 
      16S ribosomal RNA (rRNA) gene sequencing and phylogenetic analysis. B. seminalis 
      JRBHU6 also produced hydrolytic enzymes chitinases and cellulase of significance 
      in accrediting its antimicrobial nature. The bioactive metabolites produced by 
      the isolate were extracted in different organic solvents among which methanolic 
      extract showed best growth-suppressing activities toward multidrug resistant 
      Staphylococcus aureus and fungal strains, viz Fusarium oxysporum, Aspergillus 
      niger, Microsporum gypseum, Trichophyton mentagrophytes, and Trichoderma 
      harzianum. The antimicrobial compounds were purified using silica gel thin layer 
      chromatography and high-performance liquid chromatography (HPLC). On the basis of 
      spectroscopic analysis, the bioactive metabolites were identified as 
      pyrrolo(1,2-a)pyrazine-1,4-dione,hexahydro (PPDH) and 
      pyrrolo(1,2-a)pyrazine-1,4-dione, hexahydro-3(2-methylpropyl) (PPDHMP). In silico 
      molecular docking studies showed the bioactive compounds targeting fungal and 
      bacterial proteins, among which PPDHMP was multitargeting in nature as reported 
      for the first time through this study.
CI  - Copyright (c) 2021 Prasad, Pandey, Anand and Raghuwanshi.
FAU - Prasad, Jay Kishor
AU  - Prasad JK
AD  - Department of Botany, Institute of Science, Banaras Hindu University, Varanasi, 
      India.
FAU - Pandey, Priyanka
AU  - Pandey P
AD  - Department of Chemistry, Institute of Science, Banaras Hindu University, 
      Varanasi, India.
FAU - Anand, Richa
AU  - Anand R
AD  - Department of Applied Science, Indian Institute of Information 
      Technology-Allahabad, Prayagraj, India.
FAU - Raghuwanshi, Richa
AU  - Raghuwanshi R
AD  - Department of Botany, MMV, Banaras Hindu University, Varanasi, India.
LA  - eng
PT  - Journal Article
DEP - 20210414
PL  - Switzerland
TA  - Front Microbiol
JT  - Frontiers in microbiology
JID - 101548977
PMC - PMC8079638
OTO - NOTNLM
OT  - 4-dione
OT  - Burkholderia seminalis
OT  - antimicrobial activity
OT  - microbial proteins
OT  - molecular docking
OT  - pyrazine-1
OT  - spectroscopic analysis
COIS- The authors declare that the research was conducted in the absence of any 
      commercial or financial relationships that could be construed as a potential 
      conflict of interest.
EDAT- 2021/05/04 06:00
MHDA- 2021/05/04 06:01
PMCR- 2021/04/14
CRDT- 2021/05/03 06:11
PHST- 2020/12/11 00:00 [received]
PHST- 2021/02/22 00:00 [accepted]
PHST- 2021/05/03 06:11 [entrez]
PHST- 2021/05/04 06:00 [pubmed]
PHST- 2021/05/04 06:01 [medline]
PHST- 2021/04/14 00:00 [pmc-release]
AID - 10.3389/fmicb.2021.633036 [doi]
PST - epublish
SO  - Front Microbiol. 2021 Apr 14;12:633036. doi: 10.3389/fmicb.2021.633036. 
      eCollection 2021.