PMID- 33936099
OWN - NLM
STAT- MEDLINE
DCOM- 20211014
LR  - 20211014
IS  - 1664-3224 (Electronic)
IS  - 1664-3224 (Linking)
VI  - 12
DP  - 2021
TI  - Chlamydia trachomatis Infection Impairs MHC-I Intracellular Trafficking and 
      Antigen Cross-Presentation by Dendritic Cells.
PG  - 662096
LID - 10.3389/fimmu.2021.662096 [doi]
LID - 662096
AB  - During cross-presentation, exogenous antigens (i.e. intracellular pathogens or 
      tumor cells) are internalized and processed within the endocytic system and also 
      by the proteasome in the cytosol. Then, antigenic peptides are associated with 
      Major Histocompatibility Complex (MHC) class I molecules and these complexes 
      transit to the plasma membrane in order to trigger cytotoxic immune responses 
      through the activation of CD8+ T lymphocytes. Dendritic cells (DCs) are 
      particularly adapted to achieve efficient antigen cross-presentation and their 
      endocytic network displays important roles during this process, including a 
      sophisticated MHC-I transport dependent on recycling compartments. In this study, 
      we show that C. trachomatis, an obligate intracellular pathogen that exhibits 
      multiple strategies to evade the immune system, is able to induce productive 
      infections in the murine DC line JAWS-II. Our results show that when C. 
      trachomatis infects these cells, the bacteria-containing vacuole strongly 
      recruits host cell recycling vesicles, but no other endosomal compartments. 
      Furthermore, we found that chlamydial infection causes significant alterations of 
      MHC-I trafficking in JAWS-II DCs: reduced levels of MHC-I expression at the cell 
      surface, disruption of the perinuclear MHC-I intracellular pool, and impairment 
      of MHC-I endocytic recycling to the plasma membrane. We observed that all these 
      modifications lead to a hampered cross-presentation ability of soluble and 
      particulate antigens by JAWS-II DCs and primary bone marrow-derived DCs. In 
      summary, our findings provide substantial evidence that C. trachomatis hijacks 
      the DC endocytic recycling system, causing detrimental changes on MHC-I 
      intracellular transport, which are relevant for competent antigen 
      cross-presentation.
CI  - Copyright (c) 2021 Del Balzo, Capmany, Cebrian and Damiani.
FAU - Del Balzo, Diego
AU  - Del Balzo D
AD  - Biochemistry and Immunity Laboratory, School of Medicine, University of Cuyo, 
      IMBECU-CONICET, Centro Universitario, Mendoza, Argentina.
FAU - Capmany, Anahi
AU  - Capmany A
AD  - Biochemistry and Immunity Laboratory, School of Medicine, University of Cuyo, 
      IMBECU-CONICET, Centro Universitario, Mendoza, Argentina.
FAU - Cebrian, Ignacio
AU  - Cebrian I
AD  - Instituto de Histologia y Embriologia de Mendoza (IHEM)-CONICET, Facultad de 
      Ciencias Medicas, Universidad Nacional de Cuyo, Mendoza, Argentina.
FAU - Damiani, Maria Teresa
AU  - Damiani MT
AD  - Biochemistry and Immunity Laboratory, School of Medicine, University of Cuyo, 
      IMBECU-CONICET, Centro Universitario, Mendoza, Argentina.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20210415
PL  - Switzerland
TA  - Front Immunol
JT  - Frontiers in immunology
JID - 101560960
RN  - 0 (Histocompatibility Antigens Class I)
SB  - IM
MH  - Animals
MH  - Antigen Presentation/*immunology
MH  - Bone Marrow Cells/immunology
MH  - Cell Line
MH  - Chlamydia trachomatis/*immunology/pathogenicity
MH  - Cross-Priming/*immunology
MH  - Dendritic Cells/*immunology/*microbiology
MH  - Endocytosis
MH  - Histocompatibility Antigens Class I/*immunology
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Protein Transport
PMC - PMC8082151
OTO - NOTNLM
OT  - Chlamydia trachomatis
OT  - MHC-I recycling
OT  - cross-presentation
OT  - dendritic cells
OT  - endocytic recycling compartment
COIS- The authors declare that the research was conducted in the absence of any 
      commercial or financial relationships that could be construed as a potential 
      conflict of interest.
EDAT- 2021/05/04 06:00
MHDA- 2021/10/15 06:00
PMCR- 2021/01/01
CRDT- 2021/05/03 06:12
PHST- 2021/01/31 00:00 [received]
PHST- 2021/03/26 00:00 [accepted]
PHST- 2021/05/03 06:12 [entrez]
PHST- 2021/05/04 06:00 [pubmed]
PHST- 2021/10/15 06:00 [medline]
PHST- 2021/01/01 00:00 [pmc-release]
AID - 10.3389/fimmu.2021.662096 [doi]
PST - epublish
SO  - Front Immunol. 2021 Apr 15;12:662096. doi: 10.3389/fimmu.2021.662096. eCollection 
      2021.