PMID- 33936275
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20210504
IS  - 1792-0981 (Print)
IS  - 1792-1015 (Electronic)
IS  - 1792-0981 (Linking)
VI  - 21
IP  - 6
DP  - 2021 Jun
TI  - Total flavonoids of Rhizoma Drynariae combined with calcium attenuate 
      osteoporosis by reducing reactive oxygen species generation.
PG  - 618
LID - 10.3892/etm.2021.10050 [doi]
LID - 618
AB  - In the present study, the effects of total flavonoids of Rhizoma Drynariae (TFRD) 
      and calcium carbonate (CaCO(3)) on osteoporosis (OP) were assessed in a rat model 
      of OP. For this purpose, 36 Sprague-Dawley rats, aged 3 months, were randomly 
      divided into a group undergoing sham surgery (sham-operated group), model group 
      (OP group), CaCO(3) group (OP + CaCO(3) group), TFRD group (OP + TFRD group), 
      TFRD combined with CaCO(3) group (OP + TFRD + CaCO(3) group) and TFRD and CaCO(3) 
      combined with N-acetyl cysteine group (OP + TFRD + CaCO(3) + NAC group). The rat 
      model of OP was established by bilateral ovariectomy. The changes in bone mineral 
      density (BMD), bone volume parameters and bone histopathology in the rats from 
      each group were observed. The levels of serum reactive oxygen species, superoxide 
      dismutase (SOD), malondialdehyde, glutathione peroxidase (GSH-Px), interleukin 
      (IL)-6, IL-1beta, TNF-alpha, and the levels of bone tissue runt-related transcription 
      factor 2 (RUNX2), osteoprotegerin (OPG), osteocalcin (BGP), PI3K, p-PI3K, AKT, 
      p-AKT, mammalian target of rapamycin (mTOR) and p-mTOR were measured in the rats 
      of each group. The induction of OP was associated with a marked decrease in BMD, 
      bone mineral content, bone volume fraction and trabecular thickness, and 
      decreased serum levels of SOD and GSH-Px. Moreover, the expressions of RUNX2, 
      OPG, BGP were downregulated and an upregulation of p-PI3K, p-AKT and p-mTOR were 
      observed in osteoporotic rats. However, treatment with TFRD and CaCO(3) restored 
      all the aforementioned parameters to almost normal values. Furthermore, the 
      findings on histopathological evaluation were consistent with the biochemical 
      observations. Taken together, the findings of the present study demonstrated that 
      TFRD and CaCO(3) significantly increased the antioxidant capacity in rats with 
      OP, increased BMD and reduced bone mineral loss, and may be useful for the 
      prevention and treatment of OP.
CI  - Copyright: (c) Mu et al.
FAU - Mu, Panyun
AU  - Mu P
AD  - Department of Orthopedics, Hospital of Chengdu University of Traditional Chinese 
      Medicine, Chengdu, Sichuan 610075, P.R. China.
FAU - Hu, Yimei
AU  - Hu Y
AD  - Department of Orthopedics, Hospital of Chengdu University of Traditional Chinese 
      Medicine, Chengdu, Sichuan 610075, P.R. China.
FAU - Ma, Xu
AU  - Ma X
AD  - Department of Orthopedics, Hospital of Chengdu University of Traditional Chinese 
      Medicine, Chengdu, Sichuan 610075, P.R. China.
FAU - Shi, Jingru
AU  - Shi J
AD  - Department of Orthopedics, Hospital of Chengdu University of Traditional Chinese 
      Medicine, Chengdu, Sichuan 610075, P.R. China.
FAU - Zhong, Zhendong
AU  - Zhong Z
AD  - Laboratory Animal Research Institute of Sichuan Provincial People's Hospital, 
      Chengdu, Sichuan 610072, P.R. China.
FAU - Huang, Lingyuan
AU  - Huang L
AD  - Chengdu Lilai Biotechnology Co., Ltd., Chengdu, Sichuan 610041, P.R. China.
LA  - eng
PT  - Journal Article
DEP - 20210414
PL  - Greece
TA  - Exp Ther Med
JT  - Experimental and therapeutic medicine
JID - 101531947
PMC - PMC8082640
OTO - NOTNLM
OT  - osteoporosis
OT  - oxidative stress
OT  - total flavonoids of Rhizoma Drynariae
COIS- The authors declare that they have no competing interests.
EDAT- 2021/05/04 06:00
MHDA- 2021/05/04 06:01
PMCR- 2021/04/14
CRDT- 2021/05/03 06:13
PHST- 2020/01/07 00:00 [received]
PHST- 2021/02/01 00:00 [accepted]
PHST- 2021/05/03 06:13 [entrez]
PHST- 2021/05/04 06:00 [pubmed]
PHST- 2021/05/04 06:01 [medline]
PHST- 2021/04/14 00:00 [pmc-release]
AID - ETM-0-0-10050 [pii]
AID - 10.3892/etm.2021.10050 [doi]
PST - ppublish
SO  - Exp Ther Med. 2021 Jun;21(6):618. doi: 10.3892/etm.2021.10050. Epub 2021 Apr 14.