PMID- 33936347
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20231111
IS  - 1712-9532 (Print)
IS  - 1918-1493 (Electronic)
IS  - 1712-9532 (Linking)
VI  - 2021
DP  - 2021
TI  - Immunoinformatics Identification of B- and T-Cell Epitopes in the RNA-Dependent 
      RNA Polymerase of SARS-CoV-2.
PG  - 6627141
LID - 10.1155/2021/6627141 [doi]
LID - 6627141
AB  - SARS-CoV-2 (Severe acute respiratory syndrome coronavirus-2) is a newly emerged 
      beta coronavirus and etiolating agent of COVID-19. Considering the unprecedented 
      increasing number of COVID-19 cases, the World Health Organization declared a 
      public health emergency internationally on 11(th) March 2020. However, existing 
      drugs are insufficient in dealing with this contagious virus infection; 
      therefore, a vaccine is exigent to curb this pandemic disease. In the present 
      study, B- and T-cell immune epitopes were identified for RdRp (RNA-dependent RNA 
      polymerase) protein using immunoinformatic techniques, which is proved to be a 
      rapid and efficient method to explore the candidate peptide vaccine. 
      Subsequently, antigenicity and interactions with HLA (human leukocyte antigen) 
      alleles were estimated. Further, physicochemical properties, allergenicity, 
      toxicity, and stability of RdRp protein were evaluated to demonstrate the 
      specificity of the epitope candidates. Interestingly, we identified a total of 36 
      B-cell and 16 T-cell epitopes using epitopes predictive tools. Among the 
      predicted epitopes, 26 B-cell and 9 T-cell epitopes showed non-allergenic, 
      non-toxic, and highly antigenic properties. Altogether, our study revealed that 
      RdRp of SARS-CoV-2 (an epitope-based peptide fragment) can be a potentially good 
      candidate for the development of a vaccine against SARS-CoV-2.
CI  - Copyright (c) 2021 Niti Yashvardhini et al.
FAU - Yashvardhini, Niti
AU  - Yashvardhini N
AD  - Department of Microbiology, Patna Women's College, Patna 800 001, Bihar, India.
FAU - Kumar, Amit
AU  - Kumar A
AD  - Department of Botany, Patna University, Patna 800 005, Bihar, India.
FAU - Jha, Deepak Kumar
AU  - Jha DK
AUID- ORCID: 0000-0001-8079-6755
AD  - Department of Zoology, P. C. Vigyan Mahavidyalaya, Chapra, Bihar 841 301, India.
LA  - eng
PT  - Journal Article
DEP - 20210420
PL  - Egypt
TA  - Can J Infect Dis Med Microbiol
JT  - The Canadian journal of infectious diseases & medical microbiology = Journal 
      canadien des maladies infectieuses et de la microbiologie medicale
JID - 101226876
PMC - PMC8061227
COIS- The authors declare that they have no potential conflicts of interest.
EDAT- 2021/05/04 06:00
MHDA- 2021/05/04 06:01
PMCR- 2021/01/01
CRDT- 2021/05/03 06:14
PHST- 2020/12/10 00:00 [received]
PHST- 2021/03/22 00:00 [revised]
PHST- 2021/04/02 00:00 [accepted]
PHST- 2021/05/03 06:14 [entrez]
PHST- 2021/05/04 06:00 [pubmed]
PHST- 2021/05/04 06:01 [medline]
PHST- 2021/01/01 00:00 [pmc-release]
AID - 10.1155/2021/6627141 [doi]
PST - epublish
SO  - Can J Infect Dis Med Microbiol. 2021 Apr 20;2021:6627141. doi: 
      10.1155/2021/6627141. eCollection 2021.