PMID- 33938030
OWN - NLM
STAT- Publisher
LR  - 20240222
IS  - 1097-4644 (Electronic)
IS  - 0730-2312 (Linking)
DP  - 2021 May 3
TI  - GPR30 knockdown weakens the capacity of CAF in promoting prostate cancer cell 
      invasion via reducing macrophage infiltration and M2 polarization.
LID - 10.1002/jcb.29938 [doi]
AB  - Cancer-associated fibroblasts (CAFs) can promote the development and metastasis 
      of prostate cancer partly by mediating tumor-associated inflammation. An 
      increasing amount of studies have focused on the functional interactions between 
      CAFs and immune cells in the tumor microenvironment (TME). We previously reported 
      that G protein-coupled receptor 30 (GPR30) was highly expressed in prostate CAFs 
      and plays a crucial role in prostate stromal cell activation. However, the effect 
      and underlying mechanism of GPR30 expression in prostate CAFs affecting the 
      interaction between CAFs and tumor-associated macrophages (TAMs) need further 
      elucidation. Here, we found that, compared with CAF-shControl, CAF-shGPR30 
      inhibited macrophage migration through transwell migration assays, which should 
      be attributed to the decreased expression of C-X-C motif chemokine ligand 12 
      (CXCL12). In addition, macrophages treated with a culture medium of CAF-shGPR30 
      exhibited attenuated M2 polarization with downregulated M2-like markers 
      expression. Moreover, macrophages stimulated with a culture medium of CAF-shGPR30 
      were less efficient in promoting activation of fibroblast cells and invasion of 
      PCa cells. Finally, cocultured CAF-shGPR30 and macrophages suppressed PCa cell 
      invasion compared to cocultured CAF-shControl and macrophages by decreasing 
      interleukin-6 (IL-6) secretion, and this effect could be abrogated with rescue 
      expression of IL-6. Our results pinpoint the function of GPR30 in prostate CAFs 
      on regulating the CAF-TAM interaction in the TME and provide new insights into 
      PCa therapies via regulating TME.
CI  - (c) 2021 Wiley Periodicals LLC.
FAU - Zhang, Ran
AU  - Zhang R
AUID- ORCID: 0000-0002-5036-7121
AD  - Department of Biochemistry and Molecular Biology, College of Life Sciences, 
      Bioactive Materials Key Lab of Ministry of Education, Nankai University, Tianjin, 
      China.
AD  - Shandong Provincial Key Laboratory of Radiation Oncology, Cancer Research Center, 
      Shandong Cancer Hospital and Institute, Shandong First Medical University and 
      Shandong Academy of Medical Sciences, Jinan, Shandong, China.
FAU - Zong, Jiaojiao
AU  - Zong J
AD  - Department of Biochemistry and Molecular Biology, College of Life Sciences, 
      Bioactive Materials Key Lab of Ministry of Education, Nankai University, Tianjin, 
      China.
FAU - Peng, Yanfei
AU  - Peng Y
AD  - School of Integrative Medicine, Tianjin University of Traditional Chinese 
      Medicine, Tianjin, China.
FAU - Shi, Jiandang
AU  - Shi J
AD  - Department of Biochemistry and Molecular Biology, College of Life Sciences, 
      Bioactive Materials Key Lab of Ministry of Education, Nankai University, Tianjin, 
      China.
FAU - Du, Xiaoling
AU  - Du X
AD  - Department of Biochemistry and Molecular Biology, College of Life Sciences, 
      Bioactive Materials Key Lab of Ministry of Education, Nankai University, Tianjin, 
      China.
FAU - Liu, Haitao
AU  - Liu H
AD  - Shanghai First People's Hospital Shanghai Jiaotong University, Shanghai, China.
FAU - Shen, Yongmei
AU  - Shen Y
AD  - Department of Biochemistry and Molecular Biology, College of Life Sciences, 
      Bioactive Materials Key Lab of Ministry of Education, Nankai University, Tianjin, 
      China.
FAU - Cao, Jiasong
AU  - Cao J
AD  - Department of Biochemistry and Molecular Biology, College of Life Sciences, 
      Bioactive Materials Key Lab of Ministry of Education, Nankai University, Tianjin, 
      China.
FAU - Jia, Bona
AU  - Jia B
AD  - Key Laboratory of Breast Cancer Prevention and Therapy (Ministry of 
      Education), Tianjin Key Laboratory of Medical Epigenetics, Department of 
      Biochemistry and Molecular Biology, Collaborative Innovation Center of Tianjin 
      for Medical Epigenetics, Tianjin Medical University, Tianjin, China.
FAU - Liu, Feng
AU  - Liu F
AD  - Key Laboratory of Infection and Immunity of Shandong Province and Department of 
      Immunology, School of Biomedical Sciences, Shandong University, Jinan, Shandong, 
      China.
FAU - Zhang, Ju
AU  - Zhang J
AD  - Department of Biochemistry and Molecular Biology, College of Life Sciences, 
      Bioactive Materials Key Lab of Ministry of Education, Nankai University, Tianjin, 
      China.
LA  - eng
GR  - 81672527/National Natural Science Foundation of China/
GR  - 81872087/National Natural Science Foundation of China/
PT  - Journal Article
DEP - 20210503
PL  - United States
TA  - J Cell Biochem
JT  - Journal of cellular biochemistry
JID - 8205768
SB  - IM
OTO - NOTNLM
OT  - G protein-coupled receptor 30
OT  - cancer-associated fibroblasts
OT  - prostate cancer
OT  - tumor-associated macrophages
EDAT- 2021/05/04 06:00
MHDA- 2021/05/04 06:00
CRDT- 2021/05/03 06:37
PHST- 2021/02/27 00:00 [revised]
PHST- 2019/10/09 00:00 [received]
PHST- 2021/04/05 00:00 [accepted]
PHST- 2021/05/03 06:37 [entrez]
PHST- 2021/05/04 06:00 [pubmed]
PHST- 2021/05/04 06:00 [medline]
AID - 10.1002/jcb.29938 [doi]
PST - aheadofprint
SO  - J Cell Biochem. 2021 May 3. doi: 10.1002/jcb.29938.