PMID- 33946378
OWN - NLM
STAT- MEDLINE
DCOM- 20211021
LR  - 20211021
IS  - 2073-4409 (Electronic)
IS  - 2073-4409 (Linking)
VI  - 10
IP  - 5
DP  - 2021 Apr 30
TI  - Integrated microRNA and mRNA Expression Profiling Identifies Novel Targets and 
      Networks Associated with Ebstein's Anomaly.
LID - 10.3390/cells10051066 [doi]
LID - 1066
AB  - Little is known about abundance level changes of circulating microRNAs (miRNAs) 
      and messenger RNAs (mRNA) in patients with Ebstein's anomaly (EA). Here, we 
      performed an integrated analysis to identify the differentially abundant miRNAs 
      and mRNA targets and to identify the potential therapeutic targets that might be 
      involved in the mechanisms underlying EA. A large panel of human miRNA and mRNA 
      microarrays were conducted to determine the genome-wide expression profiles in 
      the blood of 16 EA patients and 16 age and gender-matched healthy control 
      volunteers (HVs). Differential abundance level of single miRNA and mRNA was 
      validated by Real-Time quantitative PCR (RT-qPCR). Enrichment analyses of altered 
      miRNA and mRNA abundance levels were identified using bioinformatics tools. 
      Altered miRNA and mRNA abundance levels were observed between EA patients and 
      HVs. Among the deregulated miRNAs and mRNAs, 76 miRNAs (49 lower abundance and 27 
      higher abundance, fold-change of >/=2) and 29 mRNAs (25 higher abundance and 4 
      lower abundance, fold-change of >/=1.5) were identified in EA patients compared to 
      HVs. Bioinformatics analysis identified 37 pairs of putative miRNA-mRNA 
      interactions. The majority of the correlations were detected between the lower 
      abundance level of miRNA and higher abundance level of mRNA, except for 
      let-7b-5p, which showed a higher abundance level and their target gene, SCRN3, 
      showed a lower abundance level. Pathway enrichment analysis of the deregulated 
      mRNAs identified 35 significant pathways that are mostly involved in signal 
      transduction and cellular interaction pathways. Our findings provide new insights 
      into a potential molecular biomarker(s) for the EA that may guide the development 
      of novel targeting therapies.
FAU - Abu-Halima, Masood
AU  - Abu-Halima M
AUID- ORCID: 0000-0001-9048-4958
AD  - Institute of Human Genetics, Saarland University, 66421 Homburg/Saar, Germany.
AD  - Department of Pediatric Cardiology, Saarland University Medical Center, 66421 
      Homburg/Saar, Germany.
FAU - Wagner, Viktoria
AU  - Wagner V
AUID- ORCID: 0000-0002-1957-0658
AD  - Institute of Human Genetics, Saarland University, 66421 Homburg/Saar, Germany.
AD  - Center for Clinical Bioinformatics, Saarland University, 66123 Saarbrucken, 
      Germany.
FAU - Becker, Lea Simone
AU  - Becker LS
AD  - Institute of Human Genetics, Saarland University, 66421 Homburg/Saar, Germany.
FAU - Ayesh, Basim M
AU  - Ayesh BM
AUID- ORCID: 0000-0001-5905-9611
AD  - Department of Laboratory Medical Sciences, Alaqsa University, Gaza 4051, 
      Palestine.
FAU - Abd El-Rahman, Mohammed
AU  - Abd El-Rahman M
AD  - Department of Pediatric Cardiology, Saarland University Medical Center, 66421 
      Homburg/Saar, Germany.
FAU - Fischer, Ulrike
AU  - Fischer U
AUID- ORCID: 0000-0002-7808-9565
AD  - Institute of Human Genetics, Saarland University, 66421 Homburg/Saar, Germany.
FAU - Meese, Eckart
AU  - Meese E
AD  - Institute of Human Genetics, Saarland University, 66421 Homburg/Saar, Germany.
FAU - Abdul-Khaliq, Hashim
AU  - Abdul-Khaliq H
AD  - Department of Pediatric Cardiology, Saarland University Medical Center, 66421 
      Homburg/Saar, Germany.
LA  - eng
GR  - 2016/Hedwig-Stalter-Stiftung/
GR  - [Nr. 01GI0601 (2014)]/Competence Network for Congenital Heart Defects/
GR  - [Nr. 81X2800112 (2015)]/German Centre for Cardiovascular Research (DZHK)/
GR  - 2021/Fordergemeinschaft Kinderherzen/Deutsche Herzstiftung/
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20210430
PL  - Switzerland
TA  - Cells
JT  - Cells
JID - 101600052
RN  - 0 (MicroRNAs)
RN  - 0 (RNA, Messenger)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Ebstein Anomaly/*genetics/metabolism
MH  - Female
MH  - *Gene Regulatory Networks
MH  - Humans
MH  - Male
MH  - MicroRNAs/*genetics/metabolism
MH  - RNA, Messenger/*genetics/metabolism
MH  - Transcriptome
PMC - PMC8146150
OTO - NOTNLM
OT  - Ebstein's anomaly
OT  - congenital heart defect
OT  - integration analysis
OT  - mRNA
OT  - microRNA
COIS- The authors declare that the research was conducted in the absence of any 
      commercial or financial relationships that could be construed as a potential 
      conflict of interest.
EDAT- 2021/05/06 06:00
MHDA- 2023/02/25 06:00
PMCR- 2021/04/30
CRDT- 2021/05/05 01:01
PHST- 2021/03/24 00:00 [received]
PHST- 2021/04/27 00:00 [revised]
PHST- 2021/04/28 00:00 [accepted]
PHST- 2021/05/05 01:01 [entrez]
PHST- 2021/05/06 06:00 [pubmed]
PHST- 2023/02/25 06:00 [medline]
PHST- 2021/04/30 00:00 [pmc-release]
AID - cells10051066 [pii]
AID - cells-10-01066 [pii]
AID - 10.3390/cells10051066 [doi]
PST - epublish
SO  - Cells. 2021 Apr 30;10(5):1066. doi: 10.3390/cells10051066.