PMID- 33949214
OWN - NLM
STAT- MEDLINE
DCOM- 20210914
LR  - 20210914
IS  - 1522-1547 (Electronic)
IS  - 0193-1857 (Linking)
VI  - 321
IP  - 1
DP  - 2021 Jul 1
TI  - Measurement of low-grade inflammation of the esophageal mucosa with electrical 
      conductivity shows promise in assessing PPI responsiveness in patients with GERD.
PG  - G29-G40
LID - 10.1152/ajpgi.00365.2020 [doi]
AB  - A device that can easily measure electrical impedance might be a helpful tool for 
      investigating the pathophysiology of gastroesophageal reflux disease. The first 
      aim of this study was to validate our newly developed bioelectrical admittance 
      measurement (BAM) through in vitro experimentation. The second aim was to 
      investigate whether evaluation of BAM by this measurement differed between 
      patients with heartburn according to their response to proton pump inhibitor 
      (PPI) therapy. Caco-2 cell monolayers and three-dimensional tissues were examined 
      by BAM using a frequency response analyzer. BAM was also used to measure the 
      impedance through cell layers. Subsequently, BAM was performed during endoscopy 
      in 41 patients experiencing heartburn without esophageal mucosal breaks. After 
      2-wk administration of 20-mg rabeprazole twice daily, patient responses to PPI 
      were classified as "good" or "poor" according to their clinical course. In each 
      patient, histological alterations and gene expression levels of inflammation 
      mediators and tight junction proteins were evaluated. Impedance profiles 
      indicated that monolayer Caco-2 cells on top of eight-layered normal human dermal 
      fibroblasts had the highest magnitude of impedance over the range of frequencies. 
      In vivo results revealed that patients with good responses to PPI displayed 
      significantly higher admittance. Severity of low-grade inflammation was 
      significantly associated with esophageal wall admittance. Moreover, esophageal 
      wall admittance may be more closely related to basal zone hyperplasia than 
      dilatation of intercellular spaces. Thus, BAM may be able to detect abnormalities 
      in the subepithelial layer of the esophagus.NEW & NOTEWORTHY Bioelectrical 
      admittance measurement is a new method to evaluate esophageal mucosal 
      permeability vertically during upper gastrointestinal endoscopy. Measurement of 
      low-grade inflammation of the esophageal mucosa with electrical conductivity 
      shows promise in assessing proton pump inhibitor responsiveness in patients with 
      gastroesophageal reflux disease. As various gastrointestinal diseases are 
      associated with changes in mucosal permeability, bioelectrical admittance 
      measurement is expected to be clinically applied to therapeutic decision-making 
      for these diseases in the future.
FAU - Manabe, Noriaki
AU  - Manabe N
AUID- ORCID: 0000-0002-5010-3708
AD  - Division of Endoscopy and Ultrasonography, Department of Clinical Pathology and 
      Laboratory Medicine, Kawasaki Medical School, Okayama, Japan.
FAU - Yamamoto, Takatoki
AU  - Yamamoto T
AD  - Department of Mechanical and Control Engineering, Graduate School of Engineering, 
      Tokyo Institute of Technology, Tokyo, Japan.
FAU - Matsusaki, Michiya
AU  - Matsusaki M
AD  - Department of Applied Chemistry, Graduate School of Engineering, Osaka 
      University, Osaka, Japan.
FAU - Akashi, Mitsuru
AU  - Akashi M
AD  - Graduate School of Frontier Biosciences, Osaka University, Osaka, Japan.
FAU - Haruma, Ken
AU  - Haruma K
AD  - Department of General Internal Medicine 2, Kawasaki Medical School, Okayama, 
      Japan.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20210505
PL  - United States
TA  - Am J Physiol Gastrointest Liver Physiol
JT  - American journal of physiology. Gastrointestinal and liver physiology
JID - 100901227
RN  - 32828355LL (Rabeprazole)
SB  - IM
MH  - Animals
MH  - Caco-2 Cells/cytology
MH  - *Electric Conductivity
MH  - Esophageal Mucosa/drug effects/physiopathology
MH  - Esophageal pH Monitoring/methods
MH  - Female
MH  - Gastroesophageal Reflux/*drug therapy/physiopathology
MH  - Humans
MH  - Inflammation/classification/diagnosis/*metabolism
MH  - Male
MH  - Mice
MH  - Middle Aged
MH  - Mucous Membrane/physiopathology
MH  - Prospective Studies
MH  - Rabeprazole/*pharmacology
OTO - NOTNLM
OT  - electrical conductivity
OT  - endoscopy
OT  - heartburn
OT  - low-grade inflammation
OT  - proton pump inhibitor
EDAT- 2021/05/06 06:00
MHDA- 2021/09/15 06:00
CRDT- 2021/05/05 08:45
PHST- 2021/05/06 06:00 [pubmed]
PHST- 2021/09/15 06:00 [medline]
PHST- 2021/05/05 08:45 [entrez]
AID - 10.1152/ajpgi.00365.2020 [doi]
PST - ppublish
SO  - Am J Physiol Gastrointest Liver Physiol. 2021 Jul 1;321(1):G29-G40. doi: 
      10.1152/ajpgi.00365.2020. Epub 2021 May 5.