PMID- 33950379
OWN - NLM
STAT- MEDLINE
DCOM- 20220623
LR  - 20220627
IS  - 1940-6029 (Electronic)
IS  - 1064-3745 (Linking)
VI  - 2454
DP  - 2022
TI  - Derivation of Clinical-Grade Induced Pluripotent Stem Cell Lines from Erythroid 
      Progenitor Cells in Xenofree Conditions.
PG  - 775-789
LID - 10.1007/7651_2021_349 [doi]
AB  - One of the major hurdles in realizing the therapeutic potential of human-induced 
      pluripotent stem cells (iPSC) is the generation of clinical-grade iPSC lines and 
      their differentiated progenies for preclinical and clinical applications. 
      Therefore, there is a need to have standardized protocols for efficient 
      generation of clinical-grade iPSC lines from easily accessible somatic cells in 
      feeder-free, xenofree GMP grade culture conditions without genomic integration of 
      the reprogramming factors. Here, we provide a detailed protocol for expansion of 
      erythroid progenitor cells from peripheral blood mononuclear cells (PBMNC) and 
      generation of iPSC lines in feeder-free and xenofree culture conditions from 
      these cells by using GMP grade reagents. With this optimized protocol, 
      clinical-grade iPSC lines can be derived from erythroid progenitor cells expanded 
      from peripheral blood, which is easy-to-access, minimally invasive, and can be 
      obtained from any donors. It will have implications in developing a large number 
      of iPSC lines from individual healthy donors, diseased patients, or donors with 
      homozygous human leukocyte antigen (HLA) for "haplobanking."
CI  - (c) 2021. Springer Science+Business Media, LLC.
FAU - Singh, Gurbind
AU  - Singh G
AD  - Centre for Stem Cell Research (A Unit of inStem, Bengaluru), Christian Medical 
      College, Vellore, India.
FAU - Manian, Kannan V
AU  - Manian KV
AD  - Centre for Stem Cell Research (A Unit of inStem, Bengaluru), Christian Medical 
      College, Vellore, India.
FAU - Premkumar, Chitra
AU  - Premkumar C
AD  - Centre for Stem Cell Research (A Unit of inStem, Bengaluru), Christian Medical 
      College, Vellore, India.
FAU - Srivastava, Alok
AU  - Srivastava A
AD  - Centre for Stem Cell Research (A Unit of inStem, Bengaluru), Christian Medical 
      College, Vellore, India.
AD  - Department of Haematology, Christian Medical College, Vellore, India.
FAU - Daniel, Dolly
AU  - Daniel D
AD  - Department of Transfusion Medicine, Christian Medical College, Vellore, India.
FAU - Velayudhan, Shaji R
AU  - Velayudhan SR
AD  - Centre for Stem Cell Research (A Unit of inStem, Bengaluru), Christian Medical 
      College, Vellore, India. rvshaji@cmcvellore.ac.in.
AD  - Department of Haematology, Christian Medical College, Vellore, India. 
      rvshaji@cmcvellore.ac.in.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Methods Mol Biol
JT  - Methods in molecular biology (Clifton, N.J.)
JID - 9214969
SB  - IM
MH  - Cell Differentiation/genetics
MH  - Cellular Reprogramming
MH  - Erythroid Precursor Cells
MH  - Humans
MH  - *Induced Pluripotent Stem Cells
MH  - Leukocytes, Mononuclear
OTO - NOTNLM
OT  - Clinical-grade human-induced pluripotent stem cells
OT  - Erythroid progenitor cells
OT  - GMP grade
OT  - Haplobanking
OT  - Reprogramming
OT  - Xenofree
EDAT- 2021/05/06 06:00
MHDA- 2022/06/24 06:00
CRDT- 2021/05/05 12:37
PHST- 2021/05/06 06:00 [pubmed]
PHST- 2022/06/24 06:00 [medline]
PHST- 2021/05/05 12:37 [entrez]
AID - 10.1007/7651_2021_349 [doi]
PST - ppublish
SO  - Methods Mol Biol. 2022;2454:775-789. doi: 10.1007/7651_2021_349.