PMID- 33952301
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20210509
IS  - 1743-7075 (Print)
IS  - 1743-7075 (Electronic)
IS  - 1743-7075 (Linking)
VI  - 18
IP  - 1
DP  - 2021 May 5
TI  - Protective effects of calorie restriction on insulin resistance and islet 
      function in STZ-induced type 2 diabetes rats.
PG  - 48
LID - 10.1186/s12986-021-00575-y [doi]
LID - 48
AB  - BACKGROUND: Caloric restriction (CR) has become increasingly attractive in the 
      treatment of type 2 diabetes mellitus (T2DM) because of the increasingly common 
      high-calorie diet and sedentary lifestyle. This study aimed to evaluate the role 
      of CR in T2DM treatment and further explore its potential molecular mechanisms. 
      METHODS: Sixty male Sprague-Dawley rats were used in this study. The diabetes 
      model was induced by 8 weeks of high-fat diet (HFD) followed by a single dose of 
      streptozotocin injection (30 mg/kg). Subsequently, the diabetic rats were fed HFD 
      at 28 g/day (diabetic control) or 20 g/day (30% CR regimen) for 20 weeks. 
      Meanwhile, normal rats fed a free standard chow diet served as the vehicle 
      control. Body mass, plasma glucose levels, and lipid profiles were monitored. 
      After diabetes-related functional tests were performed, the rats were sacrificed 
      at 10 and 20 weeks, and glucose uptake in fresh muscle was determined. In 
      addition, western blotting and immunofluorescence were used to detect alterations 
      in AKT/AS160/GLUT4 signaling. RESULTS: We found that 30% CR significantly 
      attenuated hyperglycemia and dyslipidemia, leading to alleviation of 
      glucolipotoxicity and thus protection of islet function. Insulin resistance was 
      also markedly ameliorated, as indicated by notably improved insulin tolerance and 
      homeostatic model assessment for insulin resistance (HOMA-IR). However, the 
      improvement in glucose uptake in skeletal muscle was not significant. The 
      upregulation of AKT/AS160/GLUT4 signaling in muscle induced by 30% CR also 
      attenuated gradually over time. Interestingly, the consecutive decrease in 
      AKT/AS160/GLUT4 signaling in white adipose tissue was significantly reversed by 
      30% CR. CONCLUSION: CR (30%) could protect islet function from hyperglycemia and 
      dyslipidemia, and improve insulin resistance. The mechanism by which these 
      effects occurred is likely related to the upregulation of AKT/AS160/GLUT4 
      signaling.
FAU - Zhang, Li
AU  - Zhang L
AD  - Department of Traditional Chinese Medicine, The Seventh Affiliated Hospital, Sun 
      Yat-Sen University, Shenzhen, 518107, China.
AD  - Department of Traditional Chinese Medicine, The First Affiliated Hospital, Sun 
      Yat-Sen University, Guangzhou, 518100, China.
FAU - Huang, Ying-Juan
AU  - Huang YJ
AD  - Department of Traditional Chinese Medicine, The First Affiliated Hospital, Sun 
      Yat-Sen University, Guangzhou, 518100, China.
FAU - Sun, Jia-Pan
AU  - Sun JP
AD  - Department of Traditional Chinese Medicine, The First Affiliated Hospital, Sun 
      Yat-Sen University, Guangzhou, 518100, China.
FAU - Zhang, Ting-Ying
AU  - Zhang TY
AD  - Department of Traditional Chinese Medicine, The Seventh Affiliated Hospital, Sun 
      Yat-Sen University, Shenzhen, 518107, China.
FAU - Liu, Tao-Li
AU  - Liu TL
AD  - Department of Traditional Chinese Medicine, The Seventh Affiliated Hospital, Sun 
      Yat-Sen University, Shenzhen, 518107, China.
FAU - Ke, Bin
AU  - Ke B
AD  - Department of VIP Ward, Sun Yat-Sen University Cancer Center, Guangzhou, 510080, 
      China.
FAU - Shi, Xian-Fang
AU  - Shi XF
AD  - Department of Traditional Chinese Medicine, The Seventh Affiliated Hospital, Sun 
      Yat-Sen University, Shenzhen, 518107, China.
FAU - Li, Hui
AU  - Li H
AD  - Department of Obese and Metabolic Disease, Guangzhou Panyu Hospital of Chinese 
      Medicine, Guangzhou, 511400, China.
FAU - Zhang, Geng-Peng
AU  - Zhang GP
AD  - Department of Traditional Chinese Medicine, The Seventh Affiliated Hospital, Sun 
      Yat-Sen University, Shenzhen, 518107, China.
FAU - Ye, Zhi-Yu
AU  - Ye ZY
AD  - Department of Traditional Chinese Medicine, The Seventh Affiliated Hospital, Sun 
      Yat-Sen University, Shenzhen, 518107, China.
FAU - Hu, Jianguo
AU  - Hu J
AD  - Department of Traditional Chinese Medicine, The Seventh Affiliated Hospital, Sun 
      Yat-Sen University, Shenzhen, 518107, China.
FAU - Qin, Jian
AU  - Qin J
AUID- ORCID: 0000-0001-5877-8315
AD  - Department of Traditional Chinese Medicine, The Seventh Affiliated Hospital, Sun 
      Yat-Sen University, Shenzhen, 518107, China. himybox@yeah.net.
LA  - eng
GR  - 81874498/National Natural Science Foundation of China/
GR  - SZLY2018002/Shenzhen Healthcare Research Project/
GR  - 201903010044/Science and Technology Program of Guangzhou/
GR  - GM2019020022/Traditional Chinese Medicine Research Project of Guangming District/
PT  - Journal Article
DEP - 20210505
PL  - England
TA  - Nutr Metab (Lond)
JT  - Nutrition & metabolism
JID - 101231644
PMC - PMC8097947
OTO - NOTNLM
OT  - AKT /AS160/GLUT4
OT  - Calorie restriction
OT  - Diabetes
OT  - Insulin resistance
OT  - Streptozotocin
COIS- The authors declare that they have no competing interests.
EDAT- 2021/05/07 06:00
MHDA- 2021/05/07 06:01
PMCR- 2021/05/05
CRDT- 2021/05/06 05:46
PHST- 2020/10/10 00:00 [received]
PHST- 2021/04/16 00:00 [accepted]
PHST- 2021/05/06 05:46 [entrez]
PHST- 2021/05/07 06:00 [pubmed]
PHST- 2021/05/07 06:01 [medline]
PHST- 2021/05/05 00:00 [pmc-release]
AID - 10.1186/s12986-021-00575-y [pii]
AID - 575 [pii]
AID - 10.1186/s12986-021-00575-y [doi]
PST - epublish
SO  - Nutr Metab (Lond). 2021 May 5;18(1):48. doi: 10.1186/s12986-021-00575-y.