PMID- 33953379
OWN - NLM
STAT- MEDLINE
DCOM- 20210928
LR  - 20230201
IS  - 1471-0080 (Electronic)
IS  - 1471-0072 (Linking)
VI  - 22
IP  - 8
DP  - 2021 Aug
TI  - Understanding 3D genome organization by multidisciplinary methods.
PG  - 511-528
LID - 10.1038/s41580-021-00362-w [doi]
AB  - Understanding how chromatin is folded in the nucleus is fundamental to 
      understanding its function. Although 3D genome organization has been historically 
      difficult to study owing to a lack of relevant methodologies, major technological 
      breakthroughs in genome-wide mapping of chromatin contacts and advances in 
      imaging technologies in the twenty-first century considerably improved our 
      understanding of chromosome conformation and nuclear architecture. In this 
      Review, we discuss methods of 3D genome organization analysis, including 
      sequencing-based techniques, such as Hi-C and its derivatives, Micro-C, DamID and 
      others; microscopy-based techniques, such as super-resolution imaging coupled 
      with fluorescence in situ hybridization (FISH), multiplex FISH, in situ genome 
      sequencing and live microscopy methods; and computational and modelling 
      approaches. We describe the most commonly used techniques and their contribution 
      to our current knowledge of nuclear architecture and, finally, we provide a 
      perspective on up-and-coming methods that open possibilities for future major 
      discoveries.
CI  - (c) 2021. Springer Nature Limited.
FAU - Jerkovic, Ivana
AU  - Jerkovic I
AUID- ORCID: 0000-0001-9707-8303
AD  - Institute of Human Genetics, CNRS, University of Montpellier, Montpellier, 
      France.
FAU - Cavalli, Giacomo
AU  - Cavalli G
AUID- ORCID: 0000-0003-3709-3469
AD  - Institute of Human Genetics, CNRS, University of Montpellier, Montpellier, 
      France. giacomo.cavalli@igh.cnrs.fr.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Review
DEP - 20210505
PL  - England
TA  - Nat Rev Mol Cell Biol
JT  - Nature reviews. Molecular cell biology
JID - 100962782
RN  - 0 (Chromatin)
SB  - IM
MH  - Chromatin/*chemistry/genetics/metabolism
MH  - Chromosome Mapping
MH  - Chromosomes/chemistry/genetics/metabolism
MH  - Computational Biology
MH  - *Genome
MH  - High-Throughput Nucleotide Sequencing
MH  - Humans
MH  - Microscopy
MH  - Models, Molecular
MH  - Sequence Analysis, DNA
EDAT- 2021/05/07 06:00
MHDA- 2021/09/29 06:00
CRDT- 2021/05/06 06:56
PHST- 2021/03/16 00:00 [accepted]
PHST- 2021/05/07 06:00 [pubmed]
PHST- 2021/09/29 06:00 [medline]
PHST- 2021/05/06 06:56 [entrez]
AID - 10.1038/s41580-021-00362-w [pii]
AID - 10.1038/s41580-021-00362-w [doi]
PST - ppublish
SO  - Nat Rev Mol Cell Biol. 2021 Aug;22(8):511-528. doi: 10.1038/s41580-021-00362-w. 
      Epub 2021 May 5.