PMID- 33953594
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20220422
IS  - 1178-7031 (Print)
IS  - 1178-7031 (Electronic)
IS  - 1178-7031 (Linking)
VI  - 14
DP  - 2021
TI  - Ddb1-Cullin4-Associated-Factor 1 in Macrophages Restricts the Staphylococcus 
      aureus-Induced Osteomyelitis.
PG  - 1667-1676
LID - 10.2147/JIR.S307316 [doi]
AB  - INTRODUCTION: Ddb1-cullin4-associated-factor 1 (DCAF1) is known to regulate 
      protein ubiquitination, while the roles of DCAF1 in osteomyelitis remain unknown. 
      This study aims to investigate the effects of DCAF1 deficiency in macrophages on 
      osteomyelitis and elucidate the molecular mechanism. METHODS: Staphylococcus 
      aureus-induced mouse model of osteomyelitis was established on the 
      DCAF1(fl/fl)Lyz2(cre/+) and DCAF1(fl/fl)Lyz2(+/+) (control) mice. Flow cytometry 
      was conducted to analyze the populations of adaptive and innate immune cells. 
      Lipopolysaccharides (LPS)-induced bone marrow-derived macrophages (BMDMs) were 
      established. qRT-PCR and immunoblot analysis were used to determine the levels of 
      inflammation-related biomarkers. ELISA was used to determine the release of 
      inflammatory cytokines including IL-1beta, IL-6, and TNF. RESULTS: The populations 
      of immune cells in the bone marrow and spleen were not affected due to DCAF1 
      deficiency in macrophages. DCAF1 suppressed inflammatory cytokines in LPS-induced 
      BMDMs. Additionally, DCAF1 deficiency in macrophages induced severe symptoms 
      including less bacterial load in the femur, cortical bone loss, and reactive bone 
      formation. Mechanistic study revealed that DCAF1 deficiency induced p38 
      hyperactivation. DISCUSSION: DCAF1 in macrophages suppressed the Staphylococcus 
      aureus-induced mouse model of osteomyelitis.
CI  - (c) 2021 Zong et al.
FAU - Zong, Yang
AU  - Zong Y
AD  - Department of Orthopaedic Surgery, Shanghai Jiao Tong University Affiliated Sixth 
      People's Hospital, Shanghai, 200233, People's Republic of China.
FAU - Shan, Haojie
AU  - Shan H
AD  - Department of Orthopaedic Surgery, Shanghai Jiao Tong University Affiliated Sixth 
      People's Hospital, Shanghai, 200233, People's Republic of China.
FAU - Yin, Fuli
AU  - Yin F
AD  - Department of Orthopaedic Surgery, Shanghai Jiao Tong University Affiliated Sixth 
      People's Hospital, Shanghai, 200233, People's Republic of China.
FAU - Ma, Xin
AU  - Ma X
AD  - Department of Orthopaedic Surgery, Shanghai Jiao Tong University Affiliated Sixth 
      People's Hospital, Shanghai, 200233, People's Republic of China.
FAU - Jiang, Chaolai
AU  - Jiang C
AD  - Department of Orthopaedic Surgery, Shanghai Jiao Tong University Affiliated Sixth 
      People's Hospital, Shanghai, 200233, People's Republic of China.
FAU - Wang, Nan
AU  - Wang N
AD  - Department of Emergency, The First Affiliated Hospital of Zhengzhou University, 
      Zhengzhou, Henan, 450052, People's Republic of China.
FAU - Zhou, Lihui
AU  - Zhou L
AD  - Department of Orthopaedic Surgery, Xiangshan First People's Hospital, Ningbo, 
      Zhejiang, 315700, People's Republic of China.
FAU - Lin, Yiwei
AU  - Lin Y
AD  - Department of Orthopaedic Surgery, Shanghai Jiao Tong University Affiliated Sixth 
      People's Hospital, Shanghai, 200233, People's Republic of China.
FAU - Zhou, Zubin
AU  - Zhou Z
AD  - Department of Orthopaedic Surgery, Shanghai Jiao Tong University Affiliated Sixth 
      People's Hospital, Shanghai, 200233, People's Republic of China.
FAU - Yu, Xiaowei
AU  - Yu X
AD  - Department of Orthopaedic Surgery, Shanghai Jiao Tong University Affiliated Sixth 
      People's Hospital, Shanghai, 200233, People's Republic of China.
LA  - eng
PT  - Journal Article
DEP - 20210428
PL  - New Zealand
TA  - J Inflamm Res
JT  - Journal of inflammation research
JID - 101512684
PMC - PMC8091595
OTO - NOTNLM
OT  - DCAF1
OT  - Staphylococcus aureus
OT  - inflammation
OT  - macrophages
OT  - osteomyelitis
COIS- The authors report no conflicts of interest in this work.
EDAT- 2021/05/07 06:00
MHDA- 2021/05/07 06:01
PMCR- 2021/04/28
CRDT- 2021/05/06 07:00
PHST- 2021/02/18 00:00 [received]
PHST- 2021/04/12 00:00 [accepted]
PHST- 2021/05/06 07:00 [entrez]
PHST- 2021/05/07 06:00 [pubmed]
PHST- 2021/05/07 06:01 [medline]
PHST- 2021/04/28 00:00 [pmc-release]
AID - 307316 [pii]
AID - 10.2147/JIR.S307316 [doi]
PST - epublish
SO  - J Inflamm Res. 2021 Apr 28;14:1667-1676. doi: 10.2147/JIR.S307316. eCollection 
      2021.