PMID- 33954053
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20220422
IS  - 2167-8359 (Print)
IS  - 2167-8359 (Electronic)
IS  - 2167-8359 (Linking)
VI  - 9
DP  - 2021
TI  - Bioinformatics analysis of laryngeal squamous cell carcinoma: seeking key 
      candidate genes and pathways.
PG  - e11259
LID - 10.7717/peerj.11259 [doi]
LID - e11259
AB  - BACKGROUND: Laryngeal squamous cell carcinoma (LSCC) is the second most 
      aggressive head and neck squamous cell carcinoma. Although much work has been 
      done to optimize its treatment, patients with LSCC still have poor prognosis. 
      Therefore, figuring out differentially expressed genes (DEGs) contained in the 
      progression of LSCC and employing them as potential therapeutic targets or 
      biomarkers for LSCC is extremely meaningful. METHODS: Overlapping DEGs were 
      screened from two standalone Gene Expression Omnibus datasets, and Gene Ontology 
      and Kyoto Encyclopedia of Genes and Genomes pathway enrichment analyses were 
      performed. By applying STRING and Cytoscape, a protein-protein network was built, 
      and module analysis was carried out. The hub genes were selected by maximal 
      clique centrality with the CytoHubba plugin of Cytoscape. UALCAN and GEPIA data 
      were examined to validate the gene expression findings. Moreover, the connection 
      of the hub genes with LSCC patient overall survival was studied employing The 
      Cancer Genome Atlas. Then, western blot, qRT-PCR, CCK-8, wound healing and 
      transwell assays were bring to use for further verify the key genes. RESULTS: A 
      total of 235 DEGs were recorded, including 83 upregulated and 152 downregulated 
      genes. A total of nine hub genes that displayed a high degree of connectivity 
      were selected. UALCAN and GEPIA databases verified that these genes were highly 
      expressed in LSCC tissues. High expression of the SPP1, SERPINE1 and Matrix 
      metalloproteinases 1 (MMP1) genes was connected to worse prognosis in patients 
      with LSCC, according to the GEPIA online tool. Western blot and qRT-PCR testify 
      SPP1, SERPINE1 and MMP1 were upregulated in LSCC cells. Inhibition of SPP1, 
      SERPINE1 and MMP1 suppressed cell proliferation, invasion and migration. 
      CONCLUSION: The work here identified effective and reliable diagnostic and 
      prognostic molecular biomarkers by unified bioinformatics analysis and 
      experimental verification, indicating novel and necessary therapeutic targets for 
      LSCC.
CI  - (c) 2021 Ma et al.
FAU - Ma, Jinhua
AU  - Ma J
AD  - Department of Otolaryngology, Cangzhou Central Hospital, Cangzhou, Hebei, China.
FAU - Hu, Xiaodong
AU  - Hu X
AD  - Department of Otolaryngology, Cangzhou Central Hospital, Cangzhou, Hebei, China.
FAU - Dai, Baoqiang
AU  - Dai B
AD  - Department of Otolaryngology, Cangzhou Central Hospital, Cangzhou, Hebei, China.
FAU - Wang, Qiang
AU  - Wang Q
AD  - Department of Otolaryngology, Cangzhou Central Hospital, Cangzhou, Hebei, China.
FAU - Wang, Hongqin
AU  - Wang H
AD  - Department of Otolaryngology, Cangzhou Central Hospital, Cangzhou, Hebei, China.
LA  - eng
PT  - Journal Article
DEP - 20210414
PL  - United States
TA  - PeerJ
JT  - PeerJ
JID - 101603425
PMC - PMC8052978
OTO - NOTNLM
OT  - Bioinformatics analysis
OT  - GO
OT  - KEGG
OT  - Laryngeal squamous cell carcinoma
COIS- The authors declare that they have no competing interests.
EDAT- 2021/05/07 06:00
MHDA- 2021/05/07 06:01
PMCR- 2021/04/14
CRDT- 2021/05/06 07:06
PHST- 2020/11/26 00:00 [received]
PHST- 2021/03/22 00:00 [accepted]
PHST- 2021/05/06 07:06 [entrez]
PHST- 2021/05/07 06:00 [pubmed]
PHST- 2021/05/07 06:01 [medline]
PHST- 2021/04/14 00:00 [pmc-release]
AID - 11259 [pii]
AID - 10.7717/peerj.11259 [doi]
PST - epublish
SO  - PeerJ. 2021 Apr 14;9:e11259. doi: 10.7717/peerj.11259. eCollection 2021.