PMID- 33957344
OWN - NLM
STAT- MEDLINE
DCOM- 20210520
LR  - 20210520
IS  - 1873-3778 (Electronic)
IS  - 0021-9673 (Linking)
VI  - 1647
DP  - 2021 Jun 21
TI  - Benzoic acid-modified monolithic column for separation of hydrophilic compounds 
      by capillary electrochromatography with high content of water in mobile phase.
PG  - 462166
LID - S0021-9673(21)00290-9 [pii]
LID - 10.1016/j.chroma.2021.462166 [doi]
AB  - Hydrophilic column combined with mobile phase containing high content of water is 
      a green method for the separation of polar compounds, but there are few related 
      studies, and the separation efficiency and performance of existing columns still 
      needs to be improved. In this work, a novel monolithic column for separation of 
      hydrophilic compounds under both high water content and HILIC condition, was 
      prepared by in-situ polymerization using 4-vinylbenzoic acid (VBA) and 
      1-(Acryloyloxy)-3-(methacryloyloxy)-2-propanol (AMAP) as functional monomers. The 
      poly(VBA-co-AMAP) monolithic column showed good separation performance towards 
      various polar compounds under different chromatographic conditions based on the 
      pi-interaction, hydrophobic and hydrogen bonding interactions provided by 
      4-vinylbenzoic acid functional monomer. The highest column efficiency for adenine 
      was over 2.15 x 10(5) plates m(-1) (theoretical plate, N). In addition, the 
      monolith showed good stability and reproducibility, the relative standard 
      deviations (RSDs) of retention times within days (n = 5), between days (n = 5), 
      between columns (n = 3) and between batches (n = 3) were 0.47-1.13%, 1.20-2.68%, 
      0.59-1.78% and 1.54-3.60%, respectively. This novel type of monolith has great 
      application potential in the separation of hydrophilic compounds.
CI  - Copyright (c) 2021. Published by Elsevier B.V.
FAU - Hu, Changjun
AU  - Hu C
AD  - Key Laboratory of Combinatorial Biosynthesis and Drug Discovery, Ministry of 
      Education, Hubei Province Engineering and Technology Research Center for 
      Fluorinated Pharmaceuticals, and Wuhan University School of Pharmaceutical 
      Sciences, Wuhan, 430071, China; State Key Laboratory of Transducer Technology, 
      Chinese Academy of Sciences, Beijing 100080, China.
FAU - Mao, Zhenkun
AU  - Mao Z
AD  - Key Laboratory of Combinatorial Biosynthesis and Drug Discovery, Ministry of 
      Education, Hubei Province Engineering and Technology Research Center for 
      Fluorinated Pharmaceuticals, and Wuhan University School of Pharmaceutical 
      Sciences, Wuhan, 430071, China; State Key Laboratory of Transducer Technology, 
      Chinese Academy of Sciences, Beijing 100080, China.
FAU - Li, Zhentao
AU  - Li Z
AD  - Key Laboratory of Combinatorial Biosynthesis and Drug Discovery, Ministry of 
      Education, Hubei Province Engineering and Technology Research Center for 
      Fluorinated Pharmaceuticals, and Wuhan University School of Pharmaceutical 
      Sciences, Wuhan, 430071, China; State Key Laboratory of Transducer Technology, 
      Chinese Academy of Sciences, Beijing 100080, China.
FAU - Li, Qiaoyan
AU  - Li Q
AD  - Key Laboratory of Combinatorial Biosynthesis and Drug Discovery, Ministry of 
      Education, Hubei Province Engineering and Technology Research Center for 
      Fluorinated Pharmaceuticals, and Wuhan University School of Pharmaceutical 
      Sciences, Wuhan, 430071, China; State Key Laboratory of Transducer Technology, 
      Chinese Academy of Sciences, Beijing 100080, China.
FAU - Chen, Zilin
AU  - Chen Z
AD  - Key Laboratory of Combinatorial Biosynthesis and Drug Discovery, Ministry of 
      Education, Hubei Province Engineering and Technology Research Center for 
      Fluorinated Pharmaceuticals, and Wuhan University School of Pharmaceutical 
      Sciences, Wuhan, 430071, China; State Key Laboratory of Transducer Technology, 
      Chinese Academy of Sciences, Beijing 100080, China. Electronic address: 
      chenzl@whu.edu.cn.
LA  - eng
PT  - Journal Article
DEP - 20210422
PL  - Netherlands
TA  - J Chromatogr A
JT  - Journal of chromatography. A
JID - 9318488
RN  - 059QF0KO0R (Water)
RN  - 8SKN0B0MIM (Benzoic Acid)
RN  - JAC85A2161 (Adenine)
SB  - IM
MH  - Adenine/analysis/chemistry/isolation & purification
MH  - Benzoic Acid/*chemistry
MH  - Capillary Electrochromatography/instrumentation/*methods
MH  - Hydrophobic and Hydrophilic Interactions
MH  - Reproducibility of Results
MH  - Water/chemistry
OTO - NOTNLM
OT  - Benzoic acid
OT  - Capillary electrochromatography
OT  - High content of water in mobile phase
OT  - Monolithic column
OT  - Pharmaceutical analysis
COIS- Declaration of Competing Interest The authors declared no conflict of interest.
EDAT- 2021/05/07 06:00
MHDA- 2021/05/21 06:00
CRDT- 2021/05/06 20:34
PHST- 2020/11/29 00:00 [received]
PHST- 2021/04/07 00:00 [revised]
PHST- 2021/04/12 00:00 [accepted]
PHST- 2021/05/07 06:00 [pubmed]
PHST- 2021/05/21 06:00 [medline]
PHST- 2021/05/06 20:34 [entrez]
AID - S0021-9673(21)00290-9 [pii]
AID - 10.1016/j.chroma.2021.462166 [doi]
PST - ppublish
SO  - J Chromatogr A. 2021 Jun 21;1647:462166. doi: 10.1016/j.chroma.2021.462166. Epub 
      2021 Apr 22.