PMID- 33958320
OWN - NLM
STAT- Publisher
LR  - 20240222
IS  - 2530-0180 (Electronic)
IS  - 2530-0180 (Linking)
DP  - 2021 May 3
TI  - miR-125a-5p in astrocytes attenuates peripheral neuropathy in type 2 diabetic 
      mice through targeting TRAF6.
LID - S2530-0164(21)00104-X [pii]
LID - 10.1016/j.endinu.2021.01.007 [doi]
AB  - INTRODUCTION: Elimination or blocking of astrocytes could ameliorate neuropathic 
      pain in animal models. MiR-125a-5p, expressed in astrocyte derived extracellular 
      vesicles, could mediate astrocyte function to regulate neuron communication. 
      However, the role of miR-125a-5p in DPN (diabetic peripheral neuropathy) remains 
      elusive. MATERIALS AND METHODS: Type 2 diabetic mouse (db/db) was used as DPN 
      model, which was confirmed by detection of body weight, blood glucose, mechanical 
      allodynia, thermal hyperalgesia, glial fibrillary acidic protein (GFAP) and 
      monocyte chemoattractant protein-1 (MCP-1). Astrocyte was isolated from db/db 
      mouse and then subjected to high glucose treatment. The expression of miR-125a-5p 
      in db/db mice and high glucose-induced astrocytes was examined by qRT-PCR 
      analysis. Downstream target of miR-125a-5p was clarified by luciferase reporter 
      assay. Tail vein injection of miR-125a-5p mimic into db/db mice was then 
      performed to investigate role of miR-125a-5p on DPN. RESULTS: Type 2 diabetic 
      mice showed higher body weight and blood glucose than normal db/m mice. Thermal 
      hyperalgesia and mechanical allodynia were decreased in db/db mouse compared with 
      db/m mouse, while GFAP and MCP-1 were increased in db/db mouse. High glucose 
      treatment enhanced the protein expression of GFAP and MCP-1 in astrocytes. 
      Sciatic nerve tissues in db/db mice and high glucose-induced astrocytes exhibited 
      a decrease in miR-125a-5p. Systemic administration of miR-125a-5p mimic increased 
      mechanical allodynia and thermal hyperalgesia, whereas it decreased GFAP and 
      MCP-1. TRAF6 (tumor necrosis factor receptor associated factor 6) was validated 
      as target of miR-125a-5p. CONCLUSION: MiR-125a-5p in astrocytes attenuated DPN in 
      db/db mice by up-regulation of TRAF6, which indicated the potential therapeutic 
      effect.
CI  - Copyright (c) 2021 SEEN y SED. Publicado por Elsevier Espana, S.L.U. All rights 
      reserved.
FAU - Kasimu, Aziguli
AU  - Kasimu A
AD  - Department of Pain Treatment, People's Hospital of Xinjiang Uygur Autonomous 
      Region, Urumqi City, Xinjiang Uygur Autonomous Region 830001, China.
FAU - Apizi, Xierenguli
AU  - Apizi X
AD  - Department of Pain Treatment, People's Hospital of Xinjiang Uygur Autonomous 
      Region, Urumqi City, Xinjiang Uygur Autonomous Region 830001, China.
FAU - Talifujiang, Dilibaier
AU  - Talifujiang D
AD  - Department of Pain Treatment, People's Hospital of Xinjiang Uygur Autonomous 
      Region, Urumqi City, Xinjiang Uygur Autonomous Region 830001, China.
FAU - Ma, Xin
AU  - Ma X
AD  - Department of Pain Treatment, People's Hospital of Xinjiang Uygur Autonomous 
      Region, Urumqi City, Xinjiang Uygur Autonomous Region 830001, China.
FAU - Fang, Liping
AU  - Fang L
AD  - Department of Endocrinology, Honghu People's Hospital, Jingzhou City, Hubei 
      Province 433200, China.
FAU - Zhou, Xiangling
AU  - Zhou X
AD  - Department of Neurology, Puren Hospital Affiliated to Wuhan University of Science 
      and Technology, Wuhan City, Hubei Province 430081, China. Electronic address: 
      zhouxiangling1123@163.com.
LA  - eng
LA  - spa
PT  - Journal Article
DEP - 20210503
PL  - Spain
TA  - Endocrinol Diabetes Nutr (Engl Ed)
JT  - Endocrinologia, diabetes y nutricion
JID - 101717565
SB  - IM
OTO - NOTNLM
OT  - Astrocitos
OT  - Astrocytes
OT  - Diabetic mice
OT  - Diabetic peripheral neuropathy
OT  - Neuropatia periferica diabetica
OT  - Ratones diabeticos
OT  - TRAF6
OT  - miR-125a-5p
EDAT- 2021/05/08 06:00
MHDA- 2021/05/08 06:00
CRDT- 2021/05/07 06:31
PHST- 2020/11/23 00:00 [received]
PHST- 2021/01/10 00:00 [revised]
PHST- 2021/01/14 00:00 [accepted]
PHST- 2021/05/07 06:31 [entrez]
PHST- 2021/05/08 06:00 [pubmed]
PHST- 2021/05/08 06:00 [medline]
AID - S2530-0164(21)00104-X [pii]
AID - 10.1016/j.endinu.2021.01.007 [doi]
PST - aheadofprint
SO  - Endocrinol Diabetes Nutr (Engl Ed). 2021 May 3:S2530-0164(21)00104-X. doi: 
      10.1016/j.endinu.2021.01.007.