PMID- 33959234
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20210508
IS  - 1993-0771 (Print)
IS  - 1993-078X (Electronic)
IS  - 1993-0771 (Linking)
VI  - 15
IP  - 2
DP  - 2021
TI  - Cloning and physical localization of male-biased repetitive DNA sequences in 
      Spinacia oleracea (Amaranthaceae).
PG  - 101-118
LID - 10.3897/CompCytogen.v15i2.63061 [doi]
AB  - Spinach (Spinacia oleracea Linnaeus, 1753) is an ideal material for studying 
      molecular mechanisms of early-stage sex chromosome evolution in dioecious plants. 
      Degenerate oligonucleotide-primed polymerase chain reaction (DOP-PCR) technique 
      facilitates the retrotransposon-relevant studies by enriching specific repetitive 
      DNA sequences from a micro-dissected single chromosome. We conducted genomic 
      subtractive hybridization to screen sex-biased DNA sequences by using the DOP-PCR 
      amplification products of micro-dissected spinach Y chromosome. The screening 
      yielded 55 male-biased DNA sequences with 30 576 bp in length, of which, 32 DNA 
      sequences (12 049 bp) contained repeat DNA sequences, including LTR/Copia, 
      LTR/Gypsy, simple repeats, and DNA/CMC-EnSpm. Among these repetitive DNA 
      sequences, four DNA sequences that contained a fragment of Ty3-gypsy 
      retrotransposons (SP73, SP75, SP76, and SP77) were selected as fluorescence 
      probes to hybridization on male and female spinach karyotypes. Fluorescence in 
      situ hybridization (FISH) signals of SP73 and SP75 were captured mostly on the 
      centromeres and their surrounding area for each homolog. Hybridization signals 
      primarily appeared near the putative centromeres for each homologous chromosome 
      pair by using SP76 and SP77 probes for FISH, and sporadic signals existed on the 
      long arms. Results can be served as a basis to study the function of repetitive 
      DNA sequences in sex chromosome evolution in spinach.
CI  - Jian Zhou, Shaojing Wang, Li'ang Yu, Ning Li, Shufen Li, Yulan Zhang, Ruiyun 
      Qin, Wujun Gao, Chuanliang Deng.
FAU - Zhou, Jian
AU  - Zhou J
AD  - College of Life Sciences, Henan Normal University, Xinxiang 453007, China Henan 
      Normal University Xinxiang China.
FAU - Wang, Shaojing
AU  - Wang S
AD  - College of Life Sciences, Henan Normal University, Xinxiang 453007, China Henan 
      Normal University Xinxiang China.
FAU - Yu, Li'ang
AU  - Yu L
AD  - Department of Plant Biology, University of Illinois at Urbana-Champaign, Urbana, 
      IL 61801, USA University of Illinois at Urbana-Champaign Urbana United States of 
      America.
FAU - Li, Ning
AU  - Li N
AD  - College of Life Sciences, Henan Normal University, Xinxiang 453007, China Henan 
      Normal University Xinxiang China.
FAU - Li, Shufen
AU  - Li S
AD  - College of Life Sciences, Henan Normal University, Xinxiang 453007, China Henan 
      Normal University Xinxiang China.
FAU - Zhang, Yulan
AU  - Zhang Y
AD  - College of Life Sciences, Henan Normal University, Xinxiang 453007, China Henan 
      Normal University Xinxiang China.
FAU - Qin, Ruiyun
AU  - Qin R
AD  - College of Life Sciences, Henan Normal University, Xinxiang 453007, China Henan 
      Normal University Xinxiang China.
FAU - Gao, Wujun
AU  - Gao W
AD  - College of Life Sciences, Henan Normal University, Xinxiang 453007, China Henan 
      Normal University Xinxiang China.
FAU - Deng, Chuanliang
AU  - Deng C
AUID- ORCID: 0000-0001-6132-0145
AD  - College of Life Sciences, Henan Normal University, Xinxiang 453007, China Henan 
      Normal University Xinxiang China.
LA  - eng
PT  - Journal Article
DEP - 20210423
PL  - Bulgaria
TA  - Comp Cytogenet
JT  - Comparative cytogenetics
JID - 101503807
PMC - PMC8087614
OTO - NOTNLM
OT  - FISH
OT  - Genomic subtraction hybridization
OT  - Retrotransposon
OT  - Sex chromosome evolution
OT  - Spinach
EDAT- 2021/05/08 06:00
MHDA- 2021/05/08 06:01
PMCR- 2021/04/23
CRDT- 2021/05/07 07:11
PHST- 2021/01/13 00:00 [received]
PHST- 2021/03/28 00:00 [accepted]
PHST- 2021/05/07 07:11 [entrez]
PHST- 2021/05/08 06:00 [pubmed]
PHST- 2021/05/08 06:01 [medline]
PHST- 2021/04/23 00:00 [pmc-release]
AID - 63061 [pii]
AID - 10.3897/CompCytogen.v15i2.63061 [doi]
PST - epublish
SO  - Comp Cytogenet. 2021 Apr 23;15(2):101-118. doi: 10.3897/CompCytogen.v15i2.63061. 
      eCollection 2021.