PMID- 33959757
OWN - NLM
STAT- MEDLINE
DCOM- 20211223
LR  - 20211223
IS  - 1529-7268 (Electronic)
IS  - 0006-3363 (Linking)
VI  - 105
IP  - 2
DP  - 2021 Aug 3
TI  - C1QTNF6 participates in the pathogenesis of PCOS by affecting the inflammatory 
      response of granulosa cellsdouble dagger.
PG  - 427-438
LID - 10.1093/biolre/ioab094 [doi]
AB  - Polycystic ovary syndrome (PCOS) is a common reproductive endocrine disease. It 
      has been reported that chronic low-grade inflammation might participate in its 
      pathogenesis. C1q and TNF related 6 (C1QTNF6) is a newly identified adiponectin 
      paralog associated with inflammation. The aim of the present study was to 
      investigate the role of C1QTNF6 in the development of chronic inflammation in 
      PCOS and the underlying molecular mechanism. After analyzing the expression of 
      C1QTNF6 in the serum and granulosa cells (GCs) of PCOS patients and healthy 
      controls, we verified the roles of C1QTNF6 in inflammation through 
      dehydroepiandrosterone-induced PCOS mouse models and cell models of 
      lipopolysaccharide (LPS)-induced inflammation. The results demonstrated that 
      C1QTNF6 expression in the serum and GCs of patients with PCOS was significantly 
      elevated compared with those of the controls, and similar results were observed 
      in the serum and ovary of PCOS mouse models. In PCOS mice and 
      C1QTNF6-overexpressing PCOS mice, serum levels of pro-inflammatory factors 
      including C-reactive protein (CRP), interleukin 6 (IL6), and tumor necrosis 
      factor-alpha (TNFalpha) were increased, while the opposite effects were observed when 
      C1QTNF6 was down-regulated in PCOS mice. Furthermore, C1QTNF6 overexpression 
      up-regulated the levels of TNFalpha, IL6, and CRP and activated the AKT/NF-kappaB pathway 
      in LPS-treated KGN cells, whereas C1QTNF6 knockdown and BAY-117082 (an NF-kappaB 
      inhibitor) treatment resulted in the opposite effects. Taken together, our 
      results indicate that C1QTNF6 is involved in the pathogenesis of PCOS by 
      affecting the inflammatory response via the AKT/NF-kappaB signaling pathway.
CI  - (c) The Author(s) 2021. Published by Oxford University Press on behalf of Society 
      for the Study of Reproduction. All rights reserved. For permissions, please 
      e-mail: journals.permissions@oup.com.
FAU - Yan, Sisi
AU  - Yan S
AD  - Reproductive Medical Center, Renmin Hospital of Wuhan University and Hubei Clinic 
      Research Center for Assisted Reproductive Technology and Embryonic Development, 
      Wuhan, China.
FAU - Ding, Jinli
AU  - Ding J
AD  - Reproductive Medical Center, Renmin Hospital of Wuhan University and Hubei Clinic 
      Research Center for Assisted Reproductive Technology and Embryonic Development, 
      Wuhan, China.
FAU - Zhang, Yi
AU  - Zhang Y
AD  - Reproductive Medical Center, Renmin Hospital of Wuhan University and Hubei Clinic 
      Research Center for Assisted Reproductive Technology and Embryonic Development, 
      Wuhan, China.
FAU - Wang, Jiayu
AU  - Wang J
AD  - Reproductive Medical Center, Renmin Hospital of Wuhan University and Hubei Clinic 
      Research Center for Assisted Reproductive Technology and Embryonic Development, 
      Wuhan, China.
FAU - Zhang, Sainan
AU  - Zhang S
AD  - Reproductive Medical Center, Renmin Hospital of Wuhan University and Hubei Clinic 
      Research Center for Assisted Reproductive Technology and Embryonic Development, 
      Wuhan, China.
FAU - Yin, Tailang
AU  - Yin T
AD  - Reproductive Medical Center, Renmin Hospital of Wuhan University and Hubei Clinic 
      Research Center for Assisted Reproductive Technology and Embryonic Development, 
      Wuhan, China.
FAU - Yang, Jing
AU  - Yang J
AD  - Reproductive Medical Center, Renmin Hospital of Wuhan University and Hubei Clinic 
      Research Center for Assisted Reproductive Technology and Embryonic Development, 
      Wuhan, China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Biol Reprod
JT  - Biology of reproduction
JID - 0207224
RN  - 0 (C1qTNF6 protein, human)
RN  - 9007-34-5 (Collagen)
SB  - IM
MH  - Animals
MH  - Collagen/*genetics/metabolism
MH  - Female
MH  - Granulosa Cells/pathology
MH  - Humans
MH  - Inflammation/*genetics
MH  - Mice
MH  - Polycystic Ovary Syndrome/genetics/*immunology
OTO - NOTNLM
OT  - C1QTNF6
OT  - PCOS
OT  - granulosa cells
OT  - inflammation
EDAT- 2021/05/08 06:00
MHDA- 2021/12/24 06:00
CRDT- 2021/05/07 07:18
PHST- 2021/01/17 00:00 [received]
PHST- 2021/03/31 00:00 [revised]
PHST- 2021/04/29 00:00 [accepted]
PHST- 2021/05/08 06:00 [pubmed]
PHST- 2021/12/24 06:00 [medline]
PHST- 2021/05/07 07:18 [entrez]
AID - 6270855 [pii]
AID - 10.1093/biolre/ioab094 [doi]
PST - ppublish
SO  - Biol Reprod. 2021 Aug 3;105(2):427-438. doi: 10.1093/biolre/ioab094.