PMID- 33960559
OWN - NLM
STAT- MEDLINE
DCOM- 20210614
LR  - 20210614
IS  - 1365-2184 (Electronic)
IS  - 0960-7722 (Print)
IS  - 0960-7722 (Linking)
VI  - 54
IP  - 6
DP  - 2021 Jun
TI  - Transplantation of tauroursodeoxycholic acid-inducing M2-phenotype macrophages 
      promotes an anti-neuroinflammatory effect and functional recovery after spinal 
      cord injury in rats.
PG  - e13050
LID - 10.1111/cpr.13050 [doi]
LID - e13050
AB  - OBJECTIVES: In this study, we study the transplantation of tauroursodeoxycholic 
      acid (TUDCA)-induced M2-phenotype (M2) macrophages and their ability to promote 
      anti-neuroinflammatory effects and functional recovery in a spinal cord injury 
      (SCI) model. METHODS: To this end, compared to the granulocyte-macrophage 
      colony-stimulating factor (GM-CSF), we evaluated whether TUDCA effectively 
      differentiates bone marrow-derived macrophages (BMDMs) into M2 macrophages. 
      RESULTS: The M2 expression markers in the TUDCA-treated BMDM group were increased 
      more than those in the GM-CSF-treated BMDM group. After the SCI and 
      transplantation steps, pro-inflammatory cytokine levels and the mitogen-activated 
      protein kinase (MAPK) pathway were significantly decreased in the TUDCA-induced 
      M2 group more than they were in the GM-CSF-induced M1 group and in the TUDCA 
      group. Moreover, the TUDCA-induced M2 group showed significantly enhanced tissue 
      volumes and improved motor functions compared to the GM-CSF-induced M1 group and 
      the TUDCA group. In addition, biotinylated dextran amine (BDA)-labelled 
      corticospinal tract (CST) axons and neuronal nuclei marker (NeuN) levels were 
      increased in the TUDCA-induced M2 group more than those in the GM-CSF-induced M1 
      group and the TUDCA group. CONCLUSIONS: This study demonstrates that the 
      transplantation of TUDCA-induced M2 macrophages promotes an 
      anti-neuroinflammatory effect and motor function recovery in SCI. Therefore, we 
      suggest that the transplantation of TUDCA-induced M2 macrophages represents a 
      possible alternative cell therapy for SCI.
CI  - (c) 2021 The Authors. Cell Proliferation Published by John Wiley & Sons Ltd.
FAU - Han, Gong Ho
AU  - Han GH
AD  - Department of Neurosurgery, CHA Bundang Medical Center, CHA University, 
      Seongnam-si, Korea.
AD  - Department of Biomedical Science, CHA University, Seongnam-si, Korea.
FAU - Kim, Seong Jun
AU  - Kim SJ
AD  - Department of Neurosurgery, CHA Bundang Medical Center, CHA University, 
      Seongnam-si, Korea.
AD  - Department of Biomedical Science, CHA University, Seongnam-si, Korea.
FAU - Ko, Wan-Kyu
AU  - Ko WK
AD  - Department of Neurosurgery, CHA Bundang Medical Center, CHA University, 
      Seongnam-si, Korea.
AD  - Department of Biomedical Science, CHA University, Seongnam-si, Korea.
FAU - Lee, Daye
AU  - Lee D
AD  - Department of Neurosurgery, CHA Bundang Medical Center, CHA University, 
      Seongnam-si, Korea.
AD  - Department of Biomedical Science, CHA University, Seongnam-si, Korea.
FAU - Han, In-Bo
AU  - Han IB
AD  - Department of Neurosurgery, CHA Bundang Medical Center, CHA University, 
      Seongnam-si, Korea.
FAU - Sheen, Seung Hun
AU  - Sheen SH
AD  - Department of Neurosurgery, CHA Bundang Medical Center, CHA University, 
      Seongnam-si, Korea.
FAU - Hong, Je Beom
AU  - Hong JB
AD  - Department of Neurosurgery, Kangbuk Samsung Hospital, Sungkyunkwan University 
      School of Medicine, Seoul, Korea.
FAU - Sohn, Seil
AU  - Sohn S
AUID- ORCID: 0000-0001-5724-8099
AD  - Department of Neurosurgery, CHA Bundang Medical Center, CHA University, 
      Seongnam-si, Korea.
LA  - eng
GR  - NRF-2020R1F1A1069875/the National Research Foundation of Korea (NRF)/
GR  - the Ministry of Science, ICT and future Planning/
PT  - Journal Article
DEP - 20210507
PL  - England
TA  - Cell Prolif
JT  - Cell proliferation
JID - 9105195
RN  - 516-35-8 (Taurochenodeoxycholic Acid)
RN  - 60EUX8MN5X (ursodoxicoltaurine)
SB  - IM
MH  - Animals
MH  - Cells, Cultured
MH  - Female
MH  - Inflammation/metabolism/physiopathology/therapy
MH  - Macrophages/metabolism/*transplantation
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Recovery of Function
MH  - Spinal Cord Injuries/metabolism/physiopathology/*therapy
MH  - Taurochenodeoxycholic Acid/*metabolism
PMC - PMC8168422
OTO - NOTNLM
OT  - bone marrow-derived macrophages
OT  - neuroinflammation
OT  - spinal cord injuries
OT  - tauroursodeoxycholic acid
OT  - transplantation
COIS- The authors declare no competing financial interest.
EDAT- 2021/05/08 06:00
MHDA- 2021/06/16 06:00
PMCR- 2021/05/07
CRDT- 2021/05/07 09:11
PHST- 2021/03/25 00:00 [revised]
PHST- 2021/01/27 00:00 [received]
PHST- 2021/04/13 00:00 [accepted]
PHST- 2021/05/08 06:00 [pubmed]
PHST- 2021/06/16 06:00 [medline]
PHST- 2021/05/07 09:11 [entrez]
PHST- 2021/05/07 00:00 [pmc-release]
AID - CPR13050 [pii]
AID - 10.1111/cpr.13050 [doi]
PST - ppublish
SO  - Cell Prolif. 2021 Jun;54(6):e13050. doi: 10.1111/cpr.13050. Epub 2021 May 7.