PMID- 33967583
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20231107
IS  - 1319-562X (Print)
IS  - 2213-7106 (Electronic)
IS  - 2213-7106 (Linking)
VI  - 28
IP  - 8
DP  - 2021 Aug
TI  - Antibody profile in symptomatic/asymptomatic severe acute respiratory syndrome 
      coronavirus 2 (SARS-CoV-2) infected Saudi persons.
PG  - 4677-4682
LID - 10.1016/j.sjbs.2021.04.080 [doi]
AB  - The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)-infected persons 
      could be symptomatic or asymptomatic. Asymptomatic and symptomatic patients can 
      transmit SARS-CoV-2. This study aimed to study the humoral immune response in 
      Saudis who are Covid-19 symptomatic and asymptomatic patients. We created three 
      types of enzyme-linked immunosorbant assays (ELISAs) to reveal IgG and IgM 
      antibodies (Abs) against SARS-CoV-2. The developed ELISAs were designed to detect 
      Abs against SARS-CoV-2 N, S and N + S proteins. A number of Covid-19 symptomatic 
      (1 5 3) and asymptomatic (84) RT-PCR-confirmed patient sera were used to evaluate 
      the ELISAs and to determine the IgG and IgM antibody profile in those patients. 
      The sensitivity and specificity of these ELISAs were evaluated using pre-Covid-19 
      pandemic serum samples. The results revealed the existence of anti-SARS-CoV-2 IgG 
      and IgM Abs in Covid-19 symptomatic and asymptomatic Saudi persons. The use of 
      SARS-CoV-2 N and S proteins in the same ELISA greatly increased the detectability 
      of infection. In conclusion, the Covid-19 symptomatic and asymptomatic Saudi 
      persons demonstrated both IgG and IgM antibody profile with higher titer in 
      symptomatic patients. The use of N + S proteins as antibody capture antigens 
      greatly increased the ELISA sensitivity.
CI  - (c) 2021 The Author(s).
FAU - Ibrahim, Essam H
AU  - Ibrahim EH
AD  - Biology Department, Faculty of Science, King Khalid University, P.O. Box 9004, 
      Abha 61413, Saudi Arabia.
AD  - Research Center for Advanced Materials Science (RCAMS), King Khalid University, 
      P.O. Box 9004, Abha 61413, Saudi Arabia.
AD  - Blood Products Quality Control and Research Department, National Organization for 
      Research and Control of Biologicals, Cairo, Egypt.
FAU - Alshahrani, Mohammad Y
AU  - Alshahrani MY
AD  - Department of Clinical Laboratory Sciences, College of Applied Medical Sciences, 
      King Khalid University, P.O. Box 61413, Abha 9088, Saudi Arabia.
FAU - Ghramh, Hamed A
AU  - Ghramh HA
AD  - Biology Department, Faculty of Science, King Khalid University, P.O. Box 9004, 
      Abha 61413, Saudi Arabia.
AD  - Research Center for Advanced Materials Science (RCAMS), King Khalid University, 
      P.O. Box 9004, Abha 61413, Saudi Arabia.
AD  - Unit of Bee Research and Honey Production, Faculty of Science, King Khalid 
      University, P.O. Box 9004, Abha 61413, Saudi Arabia.
FAU - Kilany, Mona
AU  - Kilany M
AD  - Research Center for Advanced Materials Science (RCAMS), King Khalid University, 
      P.O. Box 9004, Abha 61413, Saudi Arabia.
AD  - Department of Microbiology, National Organization for Drug Control and Research 
      (NODCAR), Cairo, Egypt.
LA  - eng
PT  - Journal Article
DEP - 20210503
PL  - Saudi Arabia
TA  - Saudi J Biol Sci
JT  - Saudi journal of biological sciences
JID - 101543796
PMC - PMC8091802
OTO - NOTNLM
OT  - Antibody profile
OT  - Asymptomatic
OT  - COVID-19
OT  - ELISA
OT  - SARS-CoV-2
COIS- The authors declared that there is no conflict of interest.
EDAT- 2021/05/11 06:00
MHDA- 2021/05/11 06:01
PMCR- 2021/05/03
CRDT- 2021/05/10 06:18
PHST- 2021/03/30 00:00 [received]
PHST- 2021/04/24 00:00 [revised]
PHST- 2021/04/26 00:00 [accepted]
PHST- 2021/05/11 06:00 [pubmed]
PHST- 2021/05/11 06:01 [medline]
PHST- 2021/05/10 06:18 [entrez]
PHST- 2021/05/03 00:00 [pmc-release]
AID - S1319-562X(21)00351-X [pii]
AID - 10.1016/j.sjbs.2021.04.080 [doi]
PST - ppublish
SO  - Saudi J Biol Sci. 2021 Aug;28(8):4677-4682. doi: 10.1016/j.sjbs.2021.04.080. Epub 
      2021 May 3.