PMID- 33968168
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20210513
IS  - 1792-0981 (Print)
IS  - 1792-1015 (Electronic)
IS  - 1792-0981 (Linking)
VI  - 21
IP  - 6
DP  - 2021 Jun
TI  - Secreted frizzled-related protein 4 promotes brown adipocyte differentiation.
PG  - 637
LID - 10.3892/etm.2021.10069 [doi]
LID - 637
AB  - Secreted frizzled-related protein 4 (SFRP4) is a member of the SFRP family that 
      contains a cysteine-rich domain homologous to the putative Wnt-binding site of 
      frizzled proteins. In the present report, the effects of SFRP4 on murine brown 
      adipocyte differentiation were evaluated, which exhibited an intrinsic capacity 
      to differentiate with high efficiency. Brown preadipocytes were isolated from the 
      scapular region of brown adipose tissue, which showed that the overexpression of 
      recombinant active SFRP4 protein at three concentrations (1, 10 and 100 ng/ml) 
      significantly increased the expression of adipocyte differentiation-associated 
      genes (C/EBPalpha, C/EBPbeta, UCP-1, PRDM16, PGC1alpha and GLUT4) in a dose-dependent manner 
      compared with the control group. Secondly, adiponectin protein expression was 
      significantly inhibited in a dose-independent manner, while leptin was increased 
      in brown adipocytes by incubation with the high concentration (100 ng/ml) of 
      SFRP4. Thirdly, the role of interleukin-1beta (IL-1beta) was investigated in brown 
      adipocytes and discovered that IL-1beta cannot induce SFRP4 mRNA expression in brown 
      adipocytes, similar to human islet cells. These data suggested that SFRP4-treated 
      brown adipocytes represent a valuable in vitro model for the study of 
      adipogenesis and indicated that SFRP4 served various functions during brown 
      adipocyte differentiation.
CI  - Copyright: (c) Guan et al.
FAU - Guan, Hua
AU  - Guan H
AD  - Shaanxi Key Laboratory of Ischemic Cardiovascular Diseases and Institute of Basic 
      and Translational Medicine, Xi'an Medical University, Xi'an, Shaanxi 710021, P.R. 
      China.
AD  - Department of Anesthesiology, The Fourth Military Medical University 
      Stomatological Hospital, Xi'an, Shaanxi 710032, P.R. China.
FAU - Zheng, Huiyuan
AU  - Zheng H
AD  - Shaanxi Key Laboratory of Ischemic Cardiovascular Diseases and Institute of Basic 
      and Translational Medicine, Xi'an Medical University, Xi'an, Shaanxi 710021, P.R. 
      China.
FAU - Zhang, Jin
AU  - Zhang J
AD  - Preventive Dentistry, The Fourth Military Medical University Stomatological 
      Hospital, Xi'an, Shaanxi 710032, P.R. China.
FAU - Xiang, Aoqi
AU  - Xiang A
AD  - Shaanxi Key Laboratory of Ischemic Cardiovascular Diseases and Institute of Basic 
      and Translational Medicine, Xi'an Medical University, Xi'an, Shaanxi 710021, P.R. 
      China.
FAU - Li, Yongxin
AU  - Li Y
AD  - Department of Cardiovascular Surgery, The First Affiliated Hospital of Xi'an 
      Jiaotong University, Xi'an, Shaanxi 710061, P.R. China.
FAU - Zheng, Huadong
AU  - Zheng H
AD  - Shaanxi Key Laboratory of Ischemic Cardiovascular Diseases and Institute of Basic 
      and Translational Medicine, Xi'an Medical University, Xi'an, Shaanxi 710021, P.R. 
      China.
FAU - Xu, Lixian
AU  - Xu L
AD  - Department of Anesthesiology, The Fourth Military Medical University 
      Stomatological Hospital, Xi'an, Shaanxi 710032, P.R. China.
FAU - Liu, Enqi
AU  - Liu E
AD  - Laboratory Animal Center, Xi'an Jiaotong University Health Science Center, Xi'an, 
      Shaanxi 710061, P.R. China.
FAU - Yu, Qi
AU  - Yu Q
AD  - Shaanxi Key Laboratory of Ischemic Cardiovascular Diseases and Institute of Basic 
      and Translational Medicine, Xi'an Medical University, Xi'an, Shaanxi 710021, P.R. 
      China.
LA  - eng
PT  - Journal Article
DEP - 20210415
PL  - Greece
TA  - Exp Ther Med
JT  - Experimental and therapeutic medicine
JID - 101531947
PMC - PMC8097229
OTO - NOTNLM
OT  - SFRP4
OT  - brown adipocyte
OT  - differentiation
OT  - inflammation
OT  - interleukin-1beta
COIS- The authors declare that they have no competing interests.
EDAT- 2021/05/11 06:00
MHDA- 2021/05/11 06:01
PMCR- 2021/04/15
CRDT- 2021/05/10 06:22
PHST- 2020/05/11 00:00 [received]
PHST- 2021/03/08 00:00 [accepted]
PHST- 2021/05/10 06:22 [entrez]
PHST- 2021/05/11 06:00 [pubmed]
PHST- 2021/05/11 06:01 [medline]
PHST- 2021/04/15 00:00 [pmc-release]
AID - ETM-0-0-10069 [pii]
AID - 10.3892/etm.2021.10069 [doi]
PST - ppublish
SO  - Exp Ther Med. 2021 Jun;21(6):637. doi: 10.3892/etm.2021.10069. Epub 2021 Apr 15.