PMID- 33969714
OWN - NLM
STAT- MEDLINE
DCOM- 20211119
LR  - 20220413
IS  - 1938-2723 (Electronic)
IS  - 1076-0296 (Print)
IS  - 1076-0296 (Linking)
VI  - 27
DP  - 2021 Jan-Dec
TI  - Upregulation of Inflammatory Cytokines in Pulmonary Embolism Using Biochip-Array 
      Profiling.
PG  - 10760296211013107
LID - 10.1177/10760296211013107 [doi]
LID - 10760296211013107
AB  - The complex pathophysiology of pulmonary embolism (PE) involves hemostatic 
      activation, inflammatory processes, cellular dysfunction, and hemodynamic 
      derangements. Due to the heterogeneity of this disease, risk stratification and 
      diagnosis remains challenging. Biochip-array technology provides an integrated 
      high throughput method for analyzing blood plasma samples for the simultaneous 
      measurement of multiple biomarkers for potential risk stratification. Using 
      biochip-array method, this study aimed to quantify the inflammatory biomarkers 
      such as interleukin (IL)-1alpha, IL-1beta, IL-2, IL-4, IL-6, IL-8, IL-10, vascular 
      endothelial growth factor (VEGF), interferon gamma (IFN-gamma), tumor necrosis factor 
      alpha (TNF-alpha), monocyte chemoattractant protein-1 (MCP-1), and epidermal growth 
      factor (EGF) in 109 clinically confirmed PE patients in comparison to the control 
      group comprised of plasma samples collected from 48 healthy subjects. Cytokines 
      IL-4, IL-6, IL-8, IL-10, IL-1beta, and MCP-1 demonstrated varying level of 
      significant increase (P < 0.05) in massive-risk PE patients compared to 
      submassive- and low-risk PE patients. The upregulation of inflammatory cytokines 
      in PE patients observed in this study suggest that inflammation plays an 
      important role in the overall pathophysiology of this disease. The application of 
      biochip-array technology may provide a useful approach to evaluate these 
      biomarkers to understand the pathogenesis and risk stratification of PE patients.
FAU - Bontekoe, Emily
AU  - Bontekoe E
AUID- ORCID: 0000-0002-0880-1721
AD  - Department of Pathology and Laboratory Medicine, Cardiovascular Research 
      Institute, Hemostasis and Thrombosis Research Division, Loyola University Medical 
      Center, Health Sciences Division, Maywood, IL, USA.
FAU - Brailovsky, Yevgeniy
AU  - Brailovsky Y
AUID- ORCID: 0000-0002-4811-5267
AD  - Advanced Heart Failure, Mechanical Circulatory Support, Heart Transplant, 
      Jefferson Heart Institute, Sidney Kimmel School of Medicine, Thomas Jefferson 
      University, Philadelphia, PA, USA.
FAU - Hoppensteadt, Debra
AU  - Hoppensteadt D
AUID- ORCID: 0000-0001-9342-4213
AD  - Department of Pathology and Laboratory Medicine and Department of Pharmacology 
      and Neuroscience, Cardiovascular Research Institute, Hemostasis and Thrombosis 
      Research Division, Loyola University Chicago, Health Sciences Division, Maywood, 
      IL, USA.
FAU - Bontekoe, Jack
AU  - Bontekoe J
AUID- ORCID: 0000-0003-1379-2943
AD  - Department of Pathology and Laboratory Medicine, Cardiovascular Research 
      Institute, Hemostasis and Thrombosis Research Division, Loyola University Medical 
      Center, Health Sciences Division, Maywood, IL, USA.
FAU - Siddiqui, Fakiha
AU  - Siddiqui F
AUID- ORCID: 0000-0002-2219-7049
AD  - Department of Pathology and Laboratory Medicine, Cardiovascular Research 
      Institute, Hemostasis and Thrombosis Research Division, Loyola University Medical 
      Center, Health Sciences Division, Maywood, IL, USA.
FAU - Newman, Joshua
AU  - Newman J
AD  - Division of Cardiovascular Medicine, Department of Medicine, Loyola University 
      Medical Center, Maywood, IL, USA.
FAU - Iqbal, Omer
AU  - Iqbal O
AD  - Department of Pathology and Laboratory Medicine and Department of Ophthalmology, 
      Cardiovascular Research Institute, Loyola University Medical Center, Maywood, IL, 
      USA.
FAU - Reed, Trent
AU  - Reed T
AD  - Department of Emergency Medicine, Loyola University Medical Center, Stritch 
      School of Medicine, Maywood, IL, USA.
FAU - Fareed, Jawed
AU  - Fareed J
AUID- ORCID: 0000-0003-3465-2499
AD  - Department of Pathology and Laboratory Medicine and Department of Pharmacology 
      and Neuroscience, Cardiovascular Research Institute, Hemostasis and Thrombosis 
      Research Division, Loyola University Chicago, Health Sciences Division, Maywood, 
      IL, USA.
FAU - Darki, Amir
AU  - Darki A
AUID- ORCID: 0000-0003-0144-1804
AD  - Division of Cardiology, Department of Medicine, Loyola University Medical Center, 
      Stritch School of Medicine, Maywood, IL, USA.
LA  - eng
GR  - T35 HL120835/HL/NHLBI NIH HHS/United States
PT  - Journal Article
PL  - United States
TA  - Clin Appl Thromb Hemost
JT  - Clinical and applied thrombosis/hemostasis : official journal of the 
      International Academy of Clinical and Applied Thrombosis/Hemostasis
JID - 9508125
RN  - 0 (Cytokines)
SB  - IM
MH  - Cytokines
MH  - Female
MH  - Humans
MH  - Inflammation/*metabolism
MH  - Male
MH  - Middle Aged
MH  - Protein Array Analysis/*methods
MH  - Pulmonary Embolism/*genetics/pathology
MH  - Up-Regulation
PMC - PMC8113361
OTO - NOTNLM
OT  - biochip-array
OT  - biomarkers
OT  - inflammation
OT  - pulmonary embolism
COIS- Declaration of Conflicting Interests: The author(s) declared no potential 
      conflicts of interest with respect to the research, authorship, and/or 
      publication of this article.
EDAT- 2021/05/11 06:00
MHDA- 2021/11/20 06:00
PMCR- 2021/05/10
CRDT- 2021/05/10 09:04
PHST- 2021/05/10 09:04 [entrez]
PHST- 2021/05/11 06:00 [pubmed]
PHST- 2021/11/20 06:00 [medline]
PHST- 2021/05/10 00:00 [pmc-release]
AID - 10.1177_10760296211013107 [pii]
AID - 10.1177/10760296211013107 [doi]
PST - ppublish
SO  - Clin Appl Thromb Hemost. 2021 Jan-Dec;27:10760296211013107. doi: 
      10.1177/10760296211013107.