PMID- 33971987
OWN - NLM
STAT- MEDLINE
DCOM- 20220427
LR  - 20220427
IS  - 1475-2662 (Electronic)
IS  - 0007-1145 (Linking)
VI  - 127
IP  - 6
DP  - 2022 Mar 28
TI  - Mulberry leaf activates brown adipose tissue and induces browning of inguinal 
      white adipose tissue in type 2 diabetic rats through regulating AMP-activated 
      protein kinase signalling pathway.
PG  - 810-822
LID - 10.1017/S0007114521001537 [doi]
AB  - The current epidemic of type 2 diabetes mellitus (T2DM) significantly affects 
      human health worldwide. Activation of brown adipocytes and browning of white 
      adipocytes are considered as a promising molecular target for T2DM treatment. 
      Mulberry leaf, a traditional Chinese medicine, has been demonstrated to have 
      multi-biological activities, including anti-diabetic and anti-inflammatory 
      effects. Our experimental results showed that mulberry leaf significantly 
      alleviated the disorder of glucose and lipid metabolism in T2DM rats. In 
      addition, mulberry leaf induced browning of inguinal white adipose tissue (IWAT) 
      by enhancing the expressions of brown-mark genes as well as beige-specific genes, 
      including uncoupling protein-1 (UCP1), peroxisome proliferator-activated receptor 
      gamma coactivator 1 alpha (PGC-1alpha), peroxisome proliferator-activated receptor 
      alpha (PPARalpha), PRD1-BF-1-RIZ1 homologous domain containing protein 16 (PRDM16), 
      cell death inducing DFFA-like effector A (Cidea), CD137 and transmembrane protein 
      26 (TMEM26). Mulberry leaf also activated brown adipose tissue (BAT) by 
      increasing the expressions of brown-mark genes including UCP1, PGC-1alpha, PPARalpha, 
      PRDM16 and Cidea. Moreover, mulberry leaf enhanced the expression of nuclear 
      respiratory factor 1 (NRF-1) and mitochondrial transcription factor A (TFAM) 
      genes that are responsible for mitochondrial biogenesis in IWAT and BAT. 
      Importantly, mulberry leaf also increased the expression of UCP1 and carnitine 
      palmitoyl transferase 1 (CPT-1) proteins in both IWAT and BAT via a mechanism 
      involving AMP-activated protein kinase (AMPK) and PGC-1alpha pathway. In conclusion, 
      our findings identify the role of mulberry leaf in inducing adipose browning, 
      indicating that mulberry leaf may be used as a candidate browning agent for the 
      treatment of T2DM.
FAU - Cheng, Long
AU  - Cheng L
AD  - Department of Pharmacology, School of Chinese Materia Medica, Beijing University 
      of Chinese Medicine, Beijing 102488, People's Republic of China.
FAU - Wang, Jingkang
AU  - Wang J
AD  - Department of Pharmacology, School of Chinese Materia Medica, Beijing University 
      of Chinese Medicine, Beijing 102488, People's Republic of China.
FAU - An, Yongcheng
AU  - An Y
AD  - College of Life Sciences, Beijing University of Chinese Medicine, Beijing 102488, 
      People's Republic of China.
FAU - Dai, Hongyu
AU  - Dai H
AD  - Department of Pharmacology, School of Chinese Materia Medica, Beijing University 
      of Chinese Medicine, Beijing 102488, People's Republic of China.
FAU - Duan, Yuhui
AU  - Duan Y
AD  - Department of Pharmacology, School of Chinese Materia Medica, Beijing University 
      of Chinese Medicine, Beijing 102488, People's Republic of China.
FAU - Shi, Lu
AU  - Shi L
AD  - Department of Pharmacology, School of Chinese Materia Medica, Beijing University 
      of Chinese Medicine, Beijing 102488, People's Republic of China.
FAU - Lv, Yinglan
AU  - Lv Y
AD  - Department of Pharmacology, School of Chinese Materia Medica, Beijing University 
      of Chinese Medicine, Beijing 102488, People's Republic of China.
FAU - Li, Huimin
AU  - Li H
AD  - Department of Pharmacology, School of Chinese Materia Medica, Beijing University 
      of Chinese Medicine, Beijing 102488, People's Republic of China.
FAU - Wang, Chen
AU  - Wang C
AD  - College of Life Sciences, Beijing University of Chinese Medicine, Beijing 102488, 
      People's Republic of China.
FAU - Du, Haifeng
AU  - Du H
AD  - The Third Municipal Hospital of Chengde, 067000 Chengde, People's Republic of 
      China.
FAU - Zhong, Xiaoqing
AU  - Zhong X
AD  - Guilin Sanjin Pharmaceutical Co. Ltd, Guilin 541004, People's Republic of China.
FAU - Zhao, Baosheng
AU  - Zhao B
AD  - Beijing Research Institute of Chinese Medicine, Beijing University of Chinese 
      Medicine, Beijing 100029, People's Republic of China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20210511
PL  - England
TA  - Br J Nutr
JT  - The British journal of nutrition
JID - 0372547
RN  - 0 (PPAR alpha)
RN  - 0 (Uncoupling Protein 1)
RN  - EC 2.7.11.31 (AMP-Activated Protein Kinases)
SB  - IM
MH  - AMP-Activated Protein Kinases/genetics/metabolism
MH  - Adipose Tissue, Brown/metabolism
MH  - Adipose Tissue, White/metabolism
MH  - Animals
MH  - *Diabetes Mellitus, Experimental/metabolism
MH  - *Diabetes Mellitus, Type 2/metabolism
MH  - *Morus/metabolism
MH  - PPAR alpha/metabolism
MH  - Plant Leaves
MH  - Rats
MH  - Uncoupling Protein 1/genetics/metabolism
OTO - NOTNLM
OT  - AMP-activated protein kinase signalling pathway
OT  - Brown adipose tissue
OT  - Browning of white adipose tissue
OT  - Mulberry leaf
OT  - Type 2 diabetes mellitus
OT  - White adipose tissue
EDAT- 2021/05/12 06:00
MHDA- 2022/04/28 06:00
CRDT- 2021/05/11 05:45
PHST- 2021/05/12 06:00 [pubmed]
PHST- 2022/04/28 06:00 [medline]
PHST- 2021/05/11 05:45 [entrez]
AID - S0007114521001537 [pii]
AID - 10.1017/S0007114521001537 [doi]
PST - ppublish
SO  - Br J Nutr. 2022 Mar 28;127(6):810-822. doi: 10.1017/S0007114521001537. Epub 2021 
      May 11.