PMID- 33973589
OWN - NLM
STAT- MEDLINE
DCOM- 20210618
LR  - 20210618
IS  - 1364-5528 (Electronic)
IS  - 0003-2654 (Linking)
VI  - 146
IP  - 12
DP  - 2021 Jun 14
TI  - Liposome-assisted enzyme catalysis: toward signal amplification for sensitive 
      split-type electrochemiluminescence immunoassay.
PG  - 3918-3923
LID - 10.1039/d1an00442e [doi]
AB  - Developing an efficient signal amplification strategy is very important to 
      improve the sensitivity of bioanalysis. In this paper, a liposome-assisted enzyme 
      catalysis signal amplification strategy was developed for 
      electrochemiluminescence (ECL) immunoassay of prostate specific antigen (PSA) in 
      a split-type mode. The sandwich immunoreaction occurred in a 96-well plate, and 
      glucose oxidase (GOx) encapsulated and antibody-modified liposomes were used as 
      labels. The ECL detection was carried out using a rGO-Au NP modified glassy 
      carbon electrode (GCE). The large amount of generated H2O2, i.e. the coreactant 
      of the luminol system, and the excellent catalytic behavior of rGO-Au NPs greatly 
      boosted the ECL signal, resulting in the signal amplification. The developed ECL 
      immunosensor for detecting PSA achieved a wider linear range from 1.0 x 10-13 to 
      1.0 x 10-8 g mL-1 and a detection limit of 1.7 x 10-14 g mL-1. The application of 
      the proposed strategy was demonstrated by analyzing PSA in human serum samples 
      with recoveries from 89.0% to 113.0%, and relative standard deviations (RSDs) 
      were less than 6.6%. This work provides a new horizon to expand the application 
      of liposomes for ECL bioanalysis.
FAU - Fu, Yi-Zhuo
AU  - Fu YZ
AD  - College of Chemistry and Chemical Engineering, Institute for Conservation and 
      Utilization of Agro-bioresources in Dabie Mountains, Xinyang Normal University, 
      Xinyang 464000, China. liuym9518@sina.com jtcao11@163.com.
FAU - Liu, Xiang-Mei
AU  - Liu XM
AD  - College of Chemistry and Chemical Engineering, Institute for Conservation and 
      Utilization of Agro-bioresources in Dabie Mountains, Xinyang Normal University, 
      Xinyang 464000, China. liuym9518@sina.com jtcao11@163.com.
FAU - Ma, Shu-Hui
AU  - Ma SH
AD  - Xinyang Central Hospital, Xinyang 464000, China.
FAU - Cao, Jun-Tao
AU  - Cao JT
AUID- ORCID: 0000-0002-8983-4655
AD  - College of Chemistry and Chemical Engineering, Institute for Conservation and 
      Utilization of Agro-bioresources in Dabie Mountains, Xinyang Normal University, 
      Xinyang 464000, China. liuym9518@sina.com jtcao11@163.com.
FAU - Liu, Yan-Ming
AU  - Liu YM
AUID- ORCID: 0000-0003-3381-2307
AD  - College of Chemistry and Chemical Engineering, Institute for Conservation and 
      Utilization of Agro-bioresources in Dabie Mountains, Xinyang Normal University, 
      Xinyang 464000, China. liuym9518@sina.com jtcao11@163.com.
LA  - eng
PT  - Journal Article
PL  - England
TA  - Analyst
JT  - The Analyst
JID - 0372652
RN  - 0 (Liposomes)
RN  - 7440-57-5 (Gold)
RN  - 7782-42-5 (Graphite)
RN  - BBX060AN9V (Hydrogen Peroxide)
SB  - IM
MH  - *Biosensing Techniques
MH  - Catalysis
MH  - Electrochemical Techniques
MH  - Gold
MH  - *Graphite
MH  - Humans
MH  - Hydrogen Peroxide
MH  - Immunoassay
MH  - Limit of Detection
MH  - Liposomes
MH  - Luminescent Measurements
MH  - Male
MH  - *Metal Nanoparticles
EDAT- 2021/05/12 06:00
MHDA- 2021/06/22 06:00
CRDT- 2021/05/11 09:29
PHST- 2021/05/12 06:00 [pubmed]
PHST- 2021/06/22 06:00 [medline]
PHST- 2021/05/11 09:29 [entrez]
AID - 10.1039/d1an00442e [doi]
PST - ppublish
SO  - Analyst. 2021 Jun 14;146(12):3918-3923. doi: 10.1039/d1an00442e.