PMID- 33974424
OWN - NLM
STAT- MEDLINE
DCOM- 20210527
LR  - 20210527
IS  - 1520-5118 (Electronic)
IS  - 0021-8561 (Linking)
VI  - 69
IP  - 20
DP  - 2021 May 26
TI  - Reducing the Allergenicity of alpha-Lactalbumin after Lipid Peroxidation.
PG  - 5725-5733
LID - 10.1021/acs.jafc.1c00559 [doi]
AB  - This study analyzed the effect of lipid peroxidation using 
      2,2'-azobis(2-amidinopropane)dihydrochloride (AAPH) and acrolein on the in vitro 
      and in vivo allergenicity of alpha-lactalbumin (alpha-La). The structure of oxidized alpha-La 
      was evaluated by sodium dodecyl sulfate polyacrylamide gel electrophoresis, 
      fluorescence spectroscopy, and circular dichroism, whereas the changes in the 
      allergenic properties were evaluated. Lipid peroxidation induced changes to the 
      structural properties that might destroy and/or mask alpha-La epitopes. In comparison 
      to native alpha-La, oxidation complexes caused a decrease in the immunoglobulin E 
      (IgE) binding capacity, as observed via immunoblotting. Moreover, the capacity to 
      release mediators and cytokines from KU812 cells was also greatly reduced. In 
      vivo, oxidation with AAPH and acrolein caused a significant reduction in IgE, 
      IgG, IgG1, mast cell protease 1, and plasma histamine, along with the reduction 
      of mast surface c-Kit(+) and FcepsilonRI(+) expression. Therefore, these results 
      indicate that oxidation via AAPH and acrolein can potentially reduce the 
      allergenicity of alpha-La, which can help with the better understanding of the 
      changes in allergenicity of milk allergen by lipid peroxidation.
FAU - Lv, Liangtao
AU  - Lv L
AD  - School of Public Health, Health Science Center, Shenzhen University, Shenzhen, 
      Guangdong 518060, People's Republic of China.
AD  - Department of Respiratory & Allergy, Third Affiliated Hospital of Shenzhen 
      University, Shenzhen, Guangdong 518020, People's Republic of China.
FAU - He, Kan
AU  - He K
AD  - School of Public Health, Health Science Center, Shenzhen University, Shenzhen, 
      Guangdong 518060, People's Republic of China.
FAU - Sun, Fan
AU  - Sun F
AD  - School of Public Health, Health Science Center, Shenzhen University, Shenzhen, 
      Guangdong 518060, People's Republic of China.
FAU - Lin, Xiao
AU  - Lin X
AD  - School of Public Health, Health Science Center, Shenzhen University, Shenzhen, 
      Guangdong 518060, People's Republic of China.
FAU - Ye, Liying
AU  - Ye L
AD  - School of Public Health, Health Science Center, Shenzhen University, Shenzhen, 
      Guangdong 518060, People's Republic of China.
FAU - Lyu, Yansi
AU  - Lyu Y
AD  - School of Public Health, Health Science Center, Shenzhen University, Shenzhen, 
      Guangdong 518060, People's Republic of China.
AD  - Department of Obstetricians and Gynaecologists, Shenzhen University General 
      Hospital, Shenzhen, Guangdong 518060, People's Republic of China.
FAU - Liu, Lizhong
AU  - Liu L
AD  - School of Public Health, Health Science Center, Shenzhen University, Shenzhen, 
      Guangdong 518060, People's Republic of China.
FAU - Wang, Linlin
AU  - Wang L
AD  - School of Public Health, Health Science Center, Shenzhen University, Shenzhen, 
      Guangdong 518060, People's Republic of China.
AD  - Department of Digestion, Shenzhen University General Hospital, Shenzhen, 
      Guangdong 518060, People's Republic of China.
FAU - Liu, Zhigang
AU  - Liu Z
AD  - School of Public Health, Health Science Center, Shenzhen University, Shenzhen, 
      Guangdong 518060, People's Republic of China.
FAU - Wu, Xuli
AU  - Wu X
AUID- ORCID: 0000-0003-4630-7924
AD  - School of Public Health, Health Science Center, Shenzhen University, Shenzhen, 
      Guangdong 518060, People's Republic of China.
LA  - eng
PT  - Journal Article
DEP - 20210511
PL  - United States
TA  - J Agric Food Chem
JT  - Journal of agricultural and food chemistry
JID - 0374755
RN  - 0 (Allergens)
RN  - 37341-29-0 (Immunoglobulin E)
RN  - 9013-90-5 (Lactalbumin)
SB  - IM
MH  - *Allergens
MH  - Animals
MH  - Immunoglobulin E
MH  - *Lactalbumin
MH  - Lipid Peroxidation
MH  - Milk
OTO - NOTNLM
OT  - allergenicity
OT  - cell model
OT  - lipid peroxidation
OT  - mouse model
OT  - alpha-lactalbumin
EDAT- 2021/05/12 06:00
MHDA- 2021/05/28 06:00
CRDT- 2021/05/11 17:10
PHST- 2021/05/12 06:00 [pubmed]
PHST- 2021/05/28 06:00 [medline]
PHST- 2021/05/11 17:10 [entrez]
AID - 10.1021/acs.jafc.1c00559 [doi]
PST - ppublish
SO  - J Agric Food Chem. 2021 May 26;69(20):5725-5733. doi: 10.1021/acs.jafc.1c00559. 
      Epub 2021 May 11.