PMID- 33974920
OWN - NLM
STAT- MEDLINE
DCOM- 20220114
LR  - 20220114
IS  - 1878-5867 (Electronic)
IS  - 0039-128X (Linking)
VI  - 172
DP  - 2021 Aug
TI  - Androgen and glucocorticoid receptor phosphorylation following resistance 
      exercise and pre-workout supplementation.
PG  - 108859
LID - S0039-128X(21)00071-4 [pii]
LID - 10.1016/j.steroids.2021.108859 [doi]
AB  - PURPOSE: Consumption of caffeine or caffeine containing pre-workout supplements 
      (SUPP) augments steroid hormone responses to resistance exercise (RE). However, 
      the activation of glucocorticoid (GR) and androgen receptors (AR) following RE 
      SUPP has not been investigated. The purpose of this study was to determine the 
      influence of a pre-workout supplement on AR and GR phosphorylation following RE. 
      METHODS: In a randomized, counter-balanced, double-blind, placebo-controlled, 
      within-subject crossover study, ten resistance-trained males ((X +/-SD, 
      age = 22 +/- 2.4 yrs, hgt = 175 +/- 7 cm, body mass = 84.1 +/- 11.8 kg) performed four 
      sets of 8 repetitions of barbell back squats at 75% of their 1-repetition maximum 
      (1-RM) with two minutes of rest between sets and a fifth set of barbell back 
      squats at 60% of 1-RM until concentric failure. A SUPP or flavor and color 
      matched placebo (PL) was consumed 60-minutes prior to RE. Vastus lateralis muscle 
      biopsies were obtained prior to supplementation at rest (BL), and ten minutes 
      post-exercise (POST). Biopsies were analyzed for phosphorylated GR (ser134, 
      ser211, and ser226) and phosphorylated AR (ser81, ser213, ser515, ser650) via 
      western blotting. RESULTS: pGRser134 decreased, and pGRser226 increased following 
      RE (p < 0.05) with no difference between conditions (p > 0.05). pGRser211 was 
      unchanged after RE (p > 0.05). pARser515 increased, and total AR expression 
      decreased after RE (p < 0.05) in SUPP only. Testosterone and cortisol were not 
      different between SUPP and PL at POST (p > 0.05). CONCLUSION: RE influences AR 
      and GR phosphorylation, and SUPP minimally influences this response in the early 
      recovery period.
CI  - Copyright (c) 2021 Elsevier Inc. All rights reserved.
FAU - Nicoll, Justin X
AU  - Nicoll JX
AD  - California State University, Northridge, Department of Kinesiology, Northridge, 
      CA, USA. Electronic address: justin.nicoll@csun.edu.
FAU - Fry, Andrew C
AU  - Fry AC
AD  - University of Kansas, Department of Health, Sport, and Exercise Sciences, 
      Lawrence, KS, USA. Electronic address: acfry@ku.edu.
FAU - Mosier, Eric M
AU  - Mosier EM
AD  - Northwest Missouri State University, School of Health Science and Wellness, 
      Maryville, MO, USA. Electronic address: EMOSIER@nwmissouri.edu.
LA  - eng
PT  - Journal Article
PT  - Randomized Controlled Trial
DEP - 20210508
PL  - United States
TA  - Steroids
JT  - Steroids
JID - 0404536
RN  - 0 (AR protein, human)
RN  - 0 (Receptors, Androgen)
RN  - 0 (Receptors, Glucocorticoid)
SB  - IM
MH  - Adult
MH  - Cross-Over Studies
MH  - *Dietary Supplements
MH  - Double-Blind Method
MH  - *Exercise
MH  - *Gene Expression Regulation
MH  - Humans
MH  - Male
MH  - Phosphorylation
MH  - Receptors, Androgen/genetics/*metabolism
MH  - Receptors, Glucocorticoid/genetics/*metabolism
MH  - *Resistance Training
MH  - Young Adult
OTO - NOTNLM
OT  - Cell signaling
OT  - Ergogenic
OT  - Hormones
OT  - Performance
OT  - Pre-workout supplement
EDAT- 2021/05/12 06:00
MHDA- 2022/01/15 06:00
CRDT- 2021/05/11 20:11
PHST- 2020/11/19 00:00 [received]
PHST- 2021/03/05 00:00 [revised]
PHST- 2021/04/24 00:00 [accepted]
PHST- 2021/05/12 06:00 [pubmed]
PHST- 2022/01/15 06:00 [medline]
PHST- 2021/05/11 20:11 [entrez]
AID - S0039-128X(21)00071-4 [pii]
AID - 10.1016/j.steroids.2021.108859 [doi]
PST - ppublish
SO  - Steroids. 2021 Aug;172:108859. doi: 10.1016/j.steroids.2021.108859. Epub 2021 May 
      8.