PMID- 33977670
OWN - NLM
STAT- MEDLINE
DCOM- 20210820
LR  - 20210820
IS  - 2041-1014 (Electronic)
IS  - 2041-1006 (Linking)
VI  - 36
IP  - 4
DP  - 2021 Aug
TI  - Role of D-alanylation of Streptococcus mutans lipoteichoic acid in interspecies 
      competitiveness.
PG  - 233-242
LID - 10.1111/omi.12344 [doi]
AB  - BACKGROUND: The D-alanylation of lipoteichoic acid (LTA) is essential for the 
      physiological metabolism of Streptococcus mutans (S. mutans). This study was 
      designed to investigate the influence of D-alanylation of LTA on interspecies 
      competitiveness of S. mutans. METHODS: The process of D-alanylation was blocked 
      by the inactivation of dltC. Agar competition assays, conditioned medium assays, 
      and qRT-PCR were used to evaluate the production of antimicrobial compounds in S. 
      mutans mutant. Dual-species biofilm was formed to investigate the competitiveness 
      of S. mutans mutant cocultured with S. sanguinis or S. gordonii. RESULTS: S. 
      mutans mutant could not produce antimicrobial compounds efficiently when 
      cocultured with commensal bacteria (*p < 0.05). The mutant showed compromised 
      competitiveness in dual-species biofilms. The ratio of the mutant in dual-species 
      biofilms decreased, and the terminal pH of the culture medium in mutant groups 
      (mutant+S. sanguinis/S. gordonii) was higher than that in wild-type groups 
      (*p < 0.05). Scanning electron microscope (SEM) showed weaker demineralization of 
      enamel treated with dual-species biofilms consisting of mutant and commensal 
      bacteria. CONCLUSION: D-Alanylation is involved in interspecies competitiveness 
      of S. mutans within oral biofilm by regulating mutacins and lactic acid 
      production, which may modulate the profiles of dental biofilms. Results provide 
      new insights into dental caries prevention and treatment.
CI  - (c) 2021 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.
FAU - Wu, Minjing
AU  - Wu M
AUID- ORCID: 0000-0001-9634-3582
AD  - Fujian Key Laboratory of Oral Diseases &, Fujian Provincial Engineering Research 
      Center of Oral Biomaterial & Stomatological Key lab of Fujian College and 
      University, School and Hospital of Stomatology, Fujian Medical University, 
      Fuzhou, China.
AD  - Stomatological Hospital, Southern Medical University, Guangzhou, China.
FAU - Huang, Shan
AU  - Huang S
AD  - Fujian Key Laboratory of Oral Diseases &, Fujian Provincial Engineering Research 
      Center of Oral Biomaterial & Stomatological Key lab of Fujian College and 
      University, School and Hospital of Stomatology, Fujian Medical University, 
      Fuzhou, China.
FAU - Du, Jingyun
AU  - Du J
AD  - Fujian Key Laboratory of Oral Diseases &, Fujian Provincial Engineering Research 
      Center of Oral Biomaterial & Stomatological Key lab of Fujian College and 
      University, School and Hospital of Stomatology, Fujian Medical University, 
      Fuzhou, China.
FAU - Jiang, Shan
AU  - Jiang S
AD  - School of Stomatology, Shenzhen University Health Science Center, Shenzhen, 
      China.
FAU - Cai, Zhiyu
AU  - Cai Z
AD  - Department of Stomatology, Fujian Medical University Union Hospital, Fuzhou, 
      China.
FAU - Zhan, Ling
AU  - Zhan L
AD  - Division of Pediatric Dentistry, Department of Orofacial Sciences, Department of 
      Preventive and Restorative Dental Sciences, University of California, San 
      Francisco, CA, USA.
FAU - Huang, Xiaojing
AU  - Huang X
AD  - Fujian Key Laboratory of Oral Diseases &, Fujian Provincial Engineering Research 
      Center of Oral Biomaterial & Stomatological Key lab of Fujian College and 
      University, School and Hospital of Stomatology, Fujian Medical University, 
      Fuzhou, China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20210607
PL  - Denmark
TA  - Mol Oral Microbiol
JT  - Molecular oral microbiology
JID - 101524770
RN  - 0 (Lipopolysaccharides)
RN  - 0 (Teichoic Acids)
RN  - 56411-57-5 (lipoteichoic acid)
MH  - Biofilms
MH  - *Dental Caries
MH  - Humans
MH  - Lipopolysaccharides
MH  - *Streptococcus mutans/genetics
MH  - Streptococcus sanguis
MH  - Teichoic Acids
OTO - NOTNLM
OT  - Streptococcus mutans
OT  - D-alanylation
OT  - dental caries
OT  - interspecies competitiveness
OT  - lipoteichoic acid
EDAT- 2021/05/13 06:00
MHDA- 2021/08/21 06:00
CRDT- 2021/05/12 07:29
PHST- 2021/04/24 00:00 [revised]
PHST- 2020/12/25 00:00 [received]
PHST- 2021/04/26 00:00 [accepted]
PHST- 2021/05/13 06:00 [pubmed]
PHST- 2021/08/21 06:00 [medline]
PHST- 2021/05/12 07:29 [entrez]
AID - 10.1111/omi.12344 [doi]
PST - ppublish
SO  - Mol Oral Microbiol. 2021 Aug;36(4):233-242. doi: 10.1111/omi.12344. Epub 2021 Jun 
      7.