PMID- 33977753
OWN - NLM
STAT- MEDLINE
DCOM- 20210611
LR  - 20210611
IS  - 2224-5839 (Electronic)
IS  - 2224-5820 (Linking)
VI  - 10
IP  - 5
DP  - 2021 May
TI  - Aggravation of airway inflammation in RSV-infected asthmatic mice following 
      infection-induced alteration of gut microbiota.
PG  - 5084-5097
LID - 10.21037/apm-20-2052 [doi]
AB  - BACKGROUND: This study aimed to confirm the relationship between asthma, 
      respiratory syncytial virus (RSV) infection, and the gut environment by analyzing 
      the alterations in the gut microbiota of RSV-infected asthmatic mice. METHODS: 
      Twenty-four male BALB/c mice were randomly separated into a control group (CON), 
      ovalbumin (OVA) group, and an OVA + RSV group, (n=8 mice/group). At the end of 
      experiments, we evaluated the RSV-infected asthma model using Wright-Giemsa 
      staining, histopathology and immunoglobulin E (IgE) level using enzyme-linked 
      immunosorbent assays (ELISA). Next, airway hyper-responsiveness (AHR) was 
      measured using Buxco's modular and invasive system. Furthermore, IL cytokine 
      expression were measured using ELISA. Moreover, feces were collected for 16S 
      ribosome RNA (16S rRNA) sequencing and data analysis. RESULTS: We observed that 
      the total BAL fluid lung cells in the OVA + RSV group was significantly higher 
      than other group. We revealed that the inflammatory infiltration, edema, and 
      collagen hyperplasia were more severe in the OVA + RSV group. The AHR of 
      RSV-infected mice was aggravated compared with the other groups, (P<0.05 and 
      P<0.01). We observed a higher expression of IgE, interleukin (IL)-5, IL-13, 
      IL-25, and IL-33 levels in mice from the OVA and OVA + RSV groups (P<0.05 and 
      P<0.01). The associations between Prevotellaceae_UCG_001, which is positive, and 
      IgE, IL-13, IL-33 (P<0.001), IL-5 (P<0.01), and IL-25 (P<0.05) were highly 
      significant. Lachnospiraceae_NK4A136_group is also positive and was significantly 
      associated with IgE and IL-33. Helicobacter and 
      Uncultured_Bacteroidales_bacteriumare_group, which are negative, were associated 
      with IL-25 (P<0.05). CONCLUSIONS: Our results indicated that RSV-infected mice 
      with asthma may have changes in the gut microbiota's major components and may 
      influence the mutual relationship between the core operational taxonomic units 
      (OTUs) and IgE as well as inflammatory cytokines.
FAU - Wang, Jing
AU  - Wang J
AD  - Department of Traditional Chinese Medicine, Shanghai Children's Hospital, 
      Shanghai Jiao Tong University, Shanghai, China.
FAU - Lu, Huina
AU  - Lu H
AD  - Department of Pediatrics, Chongqing Traditional Chinese Medicine Hospital, 
      Chongqing, China.
FAU - Yu, Linlin
AU  - Yu L
AD  - Department of Traditional Chinese Medicine, Shanghai Children's Hospital, 
      Shanghai Jiao Tong University, Shanghai, China.
FAU - Cheng, Weiwei
AU  - Cheng W
AD  - Department of Traditional Chinese Medicine, Shanghai Children's Hospital, 
      Shanghai Jiao Tong University, Shanghai, China.
FAU - Yan, Wuning
AU  - Yan W
AD  - Department of Traditional Chinese Medicine, Shanghai Children's Hospital, 
      Shanghai Jiao Tong University, Shanghai, China.
FAU - Jing, Xiaoping
AU  - Jing X
AD  - Department of Traditional Chinese Medicine, Shanghai Children's Hospital, 
      Shanghai Jiao Tong University, Shanghai, China.
LA  - eng
PT  - Journal Article
DEP - 20210510
PL  - China
TA  - Ann Palliat Med
JT  - Annals of palliative medicine
JID - 101585484
RN  - 0 (Cytokines)
RN  - 0 (RNA, Ribosomal, 16S)
SB  - IM
MH  - Animals
MH  - *Asthma
MH  - Cytokines
MH  - *Gastrointestinal Microbiome
MH  - Inflammation
MH  - Male
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - RNA, Ribosomal, 16S/genetics
OTO - NOTNLM
OT  - Helicobacter and Uncultured_Bacteroidales_bacterium_group
OT  - Lachnospiraceae_NK4A136_group
OT  - Prevotellaceae_UCG_001
OT  - Respiratory syncytial virus (RSV)
OT  - airway inflammation
OT  - asthma
OT  - gut microbiota
EDAT- 2021/05/13 06:00
MHDA- 2021/06/12 06:00
CRDT- 2021/05/12 08:41
PHST- 2020/10/16 00:00 [received]
PHST- 2021/03/19 00:00 [accepted]
PHST- 2021/05/13 06:00 [pubmed]
PHST- 2021/06/12 06:00 [medline]
PHST- 2021/05/12 08:41 [entrez]
AID - apm-20-2052 [pii]
AID - 10.21037/apm-20-2052 [doi]
PST - ppublish
SO  - Ann Palliat Med. 2021 May;10(5):5084-5097. doi: 10.21037/apm-20-2052. Epub 2021 
      May 10.