PMID- 33978155
OWN - NLM
STAT- MEDLINE
DCOM- 20211109
LR  - 20211109
IS  - 1943-7722 (Electronic)
IS  - 0002-9173 (Linking)
VI  - 156
IP  - 5
DP  - 2021 Oct 13
TI  - Estimation of Measurement Uncertainty of Factor Assays Using the Monte Carlo 
      Simulation.
PG  - 717-721
LID - 10.1093/ajcp/aqab022 [doi]
AB  - OBJECTIVES: We aimed to quantify the measurement uncertainty (MU) for factor 
      activities using the Monte Carlo simulation (MCS), which is a computational 
      algorithm that simulates statistical sampling to obtain numerical results through 
      complex mathematical calculations. METHODS: The uncertainties of factor V (FV), 
      factor VIII (FVIII), and factor X (FX) were simulated with two coagulation 
      testing systems: ACL TOP 750 CTS (Instrumentation Laboratory) and STA Compact 
      (Diagnostica Stago). RESULTS: When the factor activities were 74% (FV), 68% 
      (FVIII), and 89% (FX), the MUs were 3.5% (FV), 9.3% (VIII), and 2.8% (FX) for ACL 
      TOP and 8.5% (FV), 18.2% (FVII), and 6.5% (FX) for STA Compact. MCS MUs were 
      compared with MUs obtained from running actual samples (not simulated) using a 
      method called the top-down approach. The MCS MU results were interchangeable with 
      MUs from the top-down approach, except for FVIII from STA Compact. CONCLUSIONS: 
      The MCS procedure is well suited for the quantification of MUs for factor assays 
      over the entire measurement range.
CI  - (c) American Society for Clinical Pathology, 2021. All rights reserved. For 
      permissions, please e-mail: journals.permissions@oup.com.
FAU - Lim, Yong Kwan
AU  - Lim YK
AUID- ORCID: 0000-0002-4300-8964
AD  - Department of Laboratory Medicine, Chung-Ang University College of Medicine, 
      Seoul, Republic of Korea.
FAU - Kweon, Oh Joo
AU  - Kweon OJ
AD  - Department of Laboratory Medicine, Chung-Ang University College of Medicine, 
      Seoul, Republic of Korea.
FAU - Lee, Mi-Kyung
AU  - Lee MK
AD  - Department of Laboratory Medicine, Chung-Ang University College of Medicine, 
      Seoul, Republic of Korea.
FAU - Kim, Hye Ryoun
AU  - Kim HR
AD  - Department of Laboratory Medicine, Chung-Ang University College of Medicine, 
      Seoul, Republic of Korea.
LA  - eng
GR  - National Research Foundation of Korea/
GR  - NRF-2017R1A2B4011631/Ministry of Education/
PT  - Journal Article
PL  - England
TA  - Am J Clin Pathol
JT  - American journal of clinical pathology
JID - 0370470
SB  - IM
MH  - *Algorithms
MH  - Blood Coagulation Tests/*standards
MH  - Calibration
MH  - Computer Simulation
MH  - Humans
MH  - *Models, Theoretical
MH  - *Monte Carlo Method
MH  - *Uncertainty
OTO - NOTNLM
OT  - Factor assay
OT  - Measurement uncertainty
OT  - Monte Carlo simulation
EDAT- 2021/05/13 06:00
MHDA- 2021/11/10 06:00
CRDT- 2021/05/12 08:57
PHST- 2021/05/13 06:00 [pubmed]
PHST- 2021/11/10 06:00 [medline]
PHST- 2021/05/12 08:57 [entrez]
AID - 6274450 [pii]
AID - 10.1093/ajcp/aqab022 [doi]
PST - ppublish
SO  - Am J Clin Pathol. 2021 Oct 13;156(5):717-721. doi: 10.1093/ajcp/aqab022.